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Biomedical subjects

S C Gulati

Publications and source records attributed to S C Gulati.

103 records · Page 6Linked to original sources
PubMed1974

Conditions for using DNA polymerase I as an RNA-dependent DNA polymerase.

Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybrids formed with the complementary DNA are of comparable specificity and stability to those formed with complementary DNAs synthesized by viral RNA-directed DNA polymerase. Synthesis of dA-dT polymers, a common occurrence with this enzyme, can be eliminated by including distamycin in the reaction mixture.

Animals↗
PubMed1972

Particles containing RNA-instructed DNA polymerase and virus-related RNA in human breast cancers.

Human breast cancers contain an RNA related to that of mouse mammary tumor virus. In 79% of the breast malignancies examined, this type of RNA is a 70S-component encapsulated with RNA-instructed DNA polymerase in a particle possessing the density characteristics of RNA tumor viruses. Further, the DNA synthesized by the human RNA enzyme complex hybridizes specifically with the RNA of mouse mammary tumor virus. Thus, four features diagnostic of agents similar to mouse mammary tumor virus are also exhibited by a particle found with high frequency in human breast cancers. The accumulating evidence for the involvement of RNA tumor viruses in at least some human neoplasias is becoming increasingly compelling.

Adenocarcinoma↗
PubMed1972

Detection of RNA-instructed DNA polymerase and high molecular weight RNA in malignant tissue.

An experimental procedure is detailed that permits the detection of 70S RNA-directed DNA synthesis in mouse mammary carcinomas. The DNA synthesized is complementary to the RNA of the mouse mammary tumor virus by molecular hybridization, thus, completing the proof that an RNA-instructed DNA polymerase has been identified. Further, RNA-instructed DNA polymerase and its 70S RNA template are physically associated in a particle that has a density characteristic of oncornaviruses. These experiments now provide the technology required to perform similar examinations of human neoplasias.

Animals↗
PubMedDate not supplied

Neuroblastoma.

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Adolescent↗
PubMedDate not supplied

Non-Hodgkin's lymphoma.

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Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Autologous bone marrow transplantation.

Several malignancies have shown a dose-response relationship. The hematopoietic toxicity of dose escalations is often rescued by bone marrow transplantation. Prolonged disease-free survival can now be expected for patients with lymphoma, Hodgkin's disease, leukemia and breast cancer when high-dose cytotoxic therapy (high dose CT) is followed by autologous bone marrow transplant (AuBMT). Bone marrow (BM) and/or peripheral blood (PB) can be cryopreserved before high-dose CT and are utilized for stem cells rescue for AuBMT. Clinical trials of patients with lymphoma, leukemia, Hodgkin's disease, and breast cancer have proven the importance of timing the induction and high-dose CT, and properly sequenced therapy has resulted in prolonged disease-free survival in these patients. The rationale for high-dose cytotoxic therapy, methods of stem cells cryopreservation, the complications of high-dose CT, and results of treatment for various malignancies are briefly detailed with emphasis on designing future trials. Carefully designed trials have proven the usefulness of AuBMT and offer a promising approach to the future of cancer chemotherapy.

Antineoplastic Agents↗