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Biomedical subjects

S C Freni

Publications and source records attributed to S C Freni.

25 records · Page 2Linked to original sources
PubMed1976

Quantitative histological aspects of liver cirrhosis.

In human liver cirrhosis and other liver diseases relatively large nuclei are found with finely meshed chromatin and large nucleoli which do not occur in liver of individuals without liver disease. The number of tetraploid nuclei near central veins and portal areas in normal livers is not statistically significantly increased in comparison with the surrounding areas. The percentage of diploid nuclei in these livers varies from 80-95 per cent. There are definite differences in the number of adjacent microscopical fields of view with an increased degree of polyploidy when livers of normal individuals are compared with livers from patients with suspected liver disease and patients with liver cirrhosis. The increased degree of polyploidy in the last two groups can be considered as sign of regeneration.

Cell Nucleus↗
PubMedDate not supplied

Non-visual prescreening of cervical smears with a flow-through cytophotometer.

An experiment was carried out with the fluorescence impulse cytophotometer I.C.P. 11 (Phywe, Göttingen, Germany) in a routine cytology laboratory. A total of 600 cervical smears were examined with 15 test samples of body fluids and tissue cultures. The results were compared with those obtained by classical Papanicolaou cytology and clinical "follow-up". The main purpose was to find criteria for malignancy to be used as parameters in automated non-visual cytology. The main problems in the technique of preparing and measuring specimens have been discussed. Preparation of cell suspensions was time consuming; moreover, techniques were not completely controllable. A method for judging histograms was discussed. It appears that unless a better method is found for preparing suspensions of single, naked and undamaged nuclei, free from leukocytes, impulse cytophotometric measurement of DNA will remain an unreliable method for prescreening of cervical smears.

Cervix Uteri↗
PubMedDate not supplied

Preparing cell suspensions from cervical smears with pepsine and ultrasonic treatment.

Since flow-through fluorometry seemed to be a workable method for prescreening cytological material, it became important to have available a reliable method of preparing suspensions of single cells with naked nuclei. An experiment was performed with cervical smears and tissue cultures exposed to varying degrees of pepsination and ultrasonication. The results were disappointing as it appeared impossible to digest cytoplasm completely without damaging the nucleus. Ultrasonic treatment appeared to have an effect only in combination with pepsin; sonication therefore is not a useful technique for dispersion of cell clusters. Moreover, a proposal found in the literature to apply sonication to effect selective damage to leukocyte nuclei was assessed but found to be unsuccessful. Pepsin treatment and ultrasonic treatment appeared conclusively to be unreliable methods for preparing suspensions of single and naked nuclei.

Cell Nucleus↗
PubMedDate not supplied

Microhematuria found by mass screening of apparently healthy males.

Preparation of urinary sediments was adapted for mass screening purposes. The procedure, characterized by most careful collection and transfer of the sediment and by a rigidly controlled work scheme, was tested in a limited population screening program with 446 healthy male participants. A capacity of 100 samples per 12 manhours was achieved. The procedure was found effective in collecting the cellular content, but there was serious cytolysis due to a 11 hours delay between micturition and processing. An unexpected finding was the presence of erythrocytes in all the samples. In 8.8 per cent of the smears more than ten RBC/hpf were present. The significance of this feature is still in study, but it might prove to be a useful prescreening criterion in the cytologic assessment of bladder cancer in large populations.

Hematuria↗