Search PubMed⌕ Search

Biomedical subjects

S Boonpucknavig

Publications and source records attributed to S Boonpucknavig.

At least 37 records · Page 2Linked to original sources
PubMed1986

Detection of antibodies in sera from patients with Opisthorchiasis.

The indirect immunofluorescent antibody technique (IFA) was used for detection of antibodies in sera of patients with Opisthorchiasis. Antibodies to fluke worm and egg antigens were detected in 166 of 205 (81%) patients. The test showed that only the IgG class of antibodies reacting exclusively with integumental wall of the worm (AW) were positive in 46.8% (96/205), reacting only with the wall of intact eggs in 11 out of 205 (5.4%) and antibodies to both fluke and their egg antigens were present in 28.8% (59/205). In addition, 5.4% (11/205) of patients' sera were positive for autoantibodies producing a speckled antinuclear antibodies (ANA) pattern. The sera positive for only AW contained detectable autoantibodies to other cell antigens including: anti-smooth muscle antibodies of 9.4% (9/96), antimitochondrial antibodies of 3.1% (3/96), anti-liver/kidney microsomes of 1% (1/96) and anti-parietal cell antibodies of 1% (1/96). Autoantibodies were undetectable in sera from normal subjects. Among the ANA positive sera, 55% (6/11) exhibited antibodies against an extractable nuclear antigen (ENA) by a tanned red cell hemagglutination assay. This finding may suggest that the autoantibody response was due to the cross reaction between worm antigen and self antigen or it may be the result of polyclonal activation of B lymphocytes in these patients.

Adolescent↗
PubMed1985

Unusual protein pattern of Opisthorchis viverrini.

The proteins of homogenized adult Opisthorchis viverrini were separated by polyacrylamide gel electrophoresis and isoelectric focusing in the presence of detergents. By far the most abundant component(s) had a Mr of 18,000 to 19,000, represented about 47% of the total parasite protein, and did not separate in isotypes.

Actins↗
PubMed1985

IgA nephropathy associated with enteric fever.

3 cases of enteric fever (2 paratyphoid and 1 typhoid) associated with IgA nephropathy were reported. Salmonella Vi antigen was demonstrated in the glomeruli. The clinical syndrome disappeared after enteric fever was treated. Possible pathogenesis was discussed relating this intestinal infection to IgA nephropathy.

Adolescent↗
PubMed1984

Autoantibodies in sera of Thai patients with Plasmodium falciparum infection.

Serum from 98 Thai adults infected with Plasmodium falciparum were examined for the presence of autoantibodies. Malarial containing antibodies sera were also revealed positive for anti-nuclear antibodies with fluorescence speckled pattern, anti-smooth muscle antibodies, anti-mitochondria antibodies and rheumatoid factor. There was no detectable antibody to double stranded DNA. There was a significant relationship between high titre of malarial antibody and high frequency of speckled pattern of anti-nuclear factor or anti-nuclear antibodies. By the ELISA technique determination of serum antibodies against an extractable nuclear antigen (ENA) in patients with P. falciparum infection gave 43.8% (43 of 98) positive result. In addition, sera contained malarial antibodies gave positive antibodies to ENA in 50% (49 of 98) by tanned red cell haemagglutination. Among the positive sera with antibodies to ENA, they showed the presence of antibodies to both ribonucleoprotein RNAase sensitive (RNP) and ribonucleoprotein RNAase resistance (Sm). Also both of antibodies exhibited positive staining of speckled pattern of antinuclear factor. This observation indicated that malaria infection induces autoantibodies which were predominantly anti-nuclear antibodies.

Adult↗
PubMed1984

Immunological studies on acute rheumatic fever and rheumatic heart disease.

The results of immunologic studies in 27 cases of acute rheumatic fever and 11 cases of chronic rheumatic heart disease are reported. In both groups, there are elevation of peripheral B lymphocytes and depression of T lymphocytes when compared to the normal control subjects. Circulating immune complexes and autoantibodies including anti-smooth muscle antibody, antimitochondria antibody and anti-parietal cell antibody are detectable in certain cases of both groups. Deposition of IgG, beta 1c and Coxsackie antigen are demonstrable in myocardial tissue in autopsy of two cases with acute rheumatic fever in association with the detection of immune complexes in their sera. Our results together with the previous reports suggested that the Coxsackie virus are one of the causative agent of the rheumatic fever. Moreover humoral and cellular immunological reaction are involved in the pathogenesis of the disease.

Antigen-Antibody Complex↗
PubMed1983

Immunopathology of acute rheumatic fever and rheumatic heart disease. The demonstration of Coxsackie group B viral antigen in the myocardium.

Immunopathologic studies were performed on heart tissue of patients with acute rheumatic fever with carditis and chronic rheumatic heart disease. Coxsackie group B viral antigen was demonstrated in 3 heart specimens of patients clinically compatible with active rheumatic fever. In two of these the pathologic findings were compatible with acute rheumatic carditis. Immunoglobulin was detected in 2 and complement in one of these heart specimens. All of the chronic rheumatic heart specimens and the controls were negative. These findings suggested that Coxsackie group B virus may be etiologically related to the pathogenesis of acute rheumatic fever.

Acute Disease↗
PubMed1983

Immunological aspects in Plasmodium falciparum infection.

Patients with P. falciparum infection were shown to have circulating immune complexes in their sera detected by the inhibition of IgG-coated latex agglutination with RF factor and by the EAC rosette inhibition test. Low numbers of T cells and high numbers of B cells which contain immunoglobulins were demonstrated. T-cell depletion may be caused by antibody to T-lymphocytes being present in the sera of these patients.

Adolescent↗
PubMed1981

Infection of young adult mice with dengue virus type 2.

Young adult female mice, five to six weeks old, were injected intraperitoneally with 2.5 x 10(6.3) LD50 of dengue-2 virus, New Guinea C strain. The mice were killed on day 1, 2, 3, 4, 5, 6, 7, 10, 14, 21, 28, and 35 respectively. By means of the immunofluorescent antibody technique, viral antigen appeared as irregular granules in the reticuloendothelial cells of liver, lymph nodes and spleen of infected mice on the first day after inoculation and then diminished. From the fifth to sixth day of infection dengue antigen appeared again as homogeneous staining in the cytoplasm of single or groups of mononuclear cells in the lymphatic sinuses only. Later, by the third week of infection, dengue antigen could be seen in the mononuclear cells located in the marginal zone of lymphoid follicle of the spleen, the pattern of staining changing to bright spherical granules. At the same time, the deposition of immune complexes (composed of dengue antigen, mouse gamma and beta 1C globulin) could be seen in the renal glomeruli of infected mice. Serum antibody to dengue virus was found at low levels, being maximal on the 14th day after infection. Dengue virus was not isolated from the sera or from the infected organs. Granulomatous inflammation developed in lymph nodes and liver of mice infected with dengue virus and in mice inoculated with normal mouse brain suspension. Proliferative glomerular lesion was observed on day 14 after inoculation without definite abnormal urine findings.

Animals↗
PubMed1980

Chronic immunologic thrombocytopenic purpura: results of cyclophosphamide therapy before splenectomy.

Corticosteroids, cyclophosphamide, and splenectomy were employed in the treatment of 43 patients with immunologic thrombocytopenic purpura. All received corticosteroids initially; 22 patients received corticosteroids and cyclophosphamide, and six received drug treatment and underwent splenectomy. Of those receiving corticosteroids alone, 11 had excellent responses and ten had good responses. Of those receiving corticosteroids and cyclophosphamide, responses to treatment were excellent in six, good in eight, and fair in two. Of those receiving drug treatment and undergoing splenectomy, the responses to treatment were excellent in four, good in one, and fair in one. The duration required for induction of remission indicated the prognosis for patients; excellent responses were observed at 1, 2.4, and 4 months in groups treated, respectively, with corticosteroids, corticosteroids and cyclophosphamide, and drugs plus splenectomy.

Adolescent↗
PubMed1980

In vitro studies of the normal human B lymphocyte receptors for preformed soluble dengue antigen-antibody complexes.

Preformed soluble dengue antigen-antibody with or without complement complexes could bind to the surface of human lymphocytes via IgG (Fc) or complement receptors respectively. It may be that these lymphocytes were B lymphocytes not T lymphocytes. Dengue antigen or antibody alone will not attach to the surface of those cells. It is emphasized that dengue antigen-antibody complexes can form in the circulation and some will bind to the surface of the B lymphocytes in vivo and play a significant role in the pathogenesis of dengue haemorrhagic fever. In addition mice could develop glomerulonephritis by injection with preformed soluble dengue antigen-antibody complexes. These mice showed proteinuria and deposits of the immune complexes in the glomeruli of the kidneys.

Animals↗
PubMed1980

Renal involvement in human trichinosis.

A renal clinicopathologic study was made in five patients with trichinosis. The patients had mild proteinuria and abnormal urinary sediment that disappeared after the disease was under control. Creatinine clearance was normal in all cases. p-Aminohippurate clearance was decreased in two patients who had hypoalbuminemia. A renal biopsy specimen showed mesangial proliferative glomerulonephritis with deposition of immunoglobulins and C3 in the mesangial areas and along the capillary loops. Glomerular fibrin deposition was noted in one case. Deposition of hyaline material and C3 was observed in the arteriolar wall. Tubular interstitial changes were not present.

Adult↗
PubMed1979

Immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic purpura.

Direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (ITP) revealed positive staining on the surface of platelets for both immunoglobulins (Igs) and human B1C globulin in 9 specimens, for only Igs in 1 specimen and for human B1C alone in 5 specimens. The pattern of the positive immunofluorescent staining was granular. Indirect immunofluorescent staining of normal platelets in serum obtained from patients with ITP was positive for antiplatelet antibody in 9 out of 31 specimens. This suggests that platelets in patients with ITP may be damaged by an antiplatelet autoantibody acting directly on the platelet surface and/or by antigen antibody complexes binding via Fc IgG receptors on the surface of the platelets.

Adolescent↗
PubMed1979

The pattern and nature of the lymphocyte population response in dengue hemorrhagic fever.

Eighty-eight specimens consisting of lymphocytes separated from peripheral blood samples from 76 patients with dengue hemorrhagic fever (DHF) were studied. The results revealed a significant increase in numbers of atypical lymphocytes and B lymphocytes, and a decrease in T lymphocytes by comparison with normal controls. Anti-T lymphocyte antibody was also detected in the serum of all patients with DHF. The greatest increments and decrements of the above were noted on the day of shock of subsidence of fever.

Adolescent↗