Biomedical subjects
S Bliss
Publications and source records attributed to S Bliss.
What is your diagnosis? Primary splenic tumor and suppurative peritonitis due to gallbladder rupture.
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Surgical gloves--a comparative study of the incidence and site of punctures.
Five surgical teams in two operating suites were studied for three months with random allocation to receive either A or B operating gloves. The gloves were identified as used by the surgeon, the assistant and the scrub nurse. At the end of each day the gloves were tested by standard air insufflation and immersion under water. Surgeons experienced significantly more punctures 174:884 (19.7%) [17%-23%] than the scrub nurses or assistants 161:1770 (9.4%) [8.6%-10.2%]. Surgeons were particularly prone to experience punctures of the tip of the index or little finger of the left hand whereas nurses were more likely to experience punctures of the index finger and thumb of the left hand. There was a wide variation between the five surgical teams varying between 5.7% [2%-11%] and 37.5% [24%-52%] wearing B and 11.4% and 28.4% wearing A. There were significantly more punctures when wearing A gloves (23.0%) [20%-26%] than when wearing B gloves (15.8%) [13%-19%] for surgeons but no significant difference for surgical assistants or scrub nurses. Contrary to our expectation there were no significant differences between the two types in the tearing of cuffs (1% in each).
Genetic exchange across a paracentric inversion of the mouse t complex.
Mouse t haplotypes are distinguished from wild-type forms of chromosome 17 by four nonoverlapping paracentric inversions which span a genetic distance of 20 cM. These inversion polymorphisms are responsible for a 100-200-fold suppression of recombination which maintains the integrity of complete t haplotypes and has led to their divergence from the wild-type chromosomes of four species of house mice within which t haplotypes reside. As evidence for the long period of recombinational isolation, alleles that distinguish all t haplotypes from all wild-type chromosomes have been established at a number of loci spread across the 20-cM variant region. However, a more complex picture emerges upon analysis of other t-associated loci. In particular, "mosaic haplotypes" have been identified that carry a mixture of wild-type and t-specific alleles. To investigate the genetic basis for mosaic chromosomes, we conducted a comprehensive analysis of eight t complex loci within 76 animals representing 10 taxa in the genus Mus, and including 23 previously characterized t haplotypes. Higher resolution restriction mapping and sequence analysis was also performed for alleles at the Hba-ps4 locus. The results indicate that a short tract of DNA was transferred relatively recently across an inversion from a t haplotype allele of Hba-ps4 to the corresponding locus on a wild-type homolog leading to the creation of a new hybrid allele. Several classes of wild-type Hba-ps4 alleles, including the most common form in inbred strains, appear to be derived from this hybrid allele. The accumulated data suggest that a common form of genetic exchange across one of the four t-associated inversions is gene conversion at isolated loci that do not play a role in the transmission ratio distortion phenotype required for t haplotype propagation. The implications of the results pose questions concerning the evolutionary stability of gene complexes within large paracentric inversions and suggest that recombinational isolation may be best established for loci residing within a short distance from inversion breakpoints.