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Biomedical subjects

S Bhattacharya

Publications and source records attributed to S Bhattacharya.

At least 397 records · Page 22Linked to original sources
PubMed1992

Purification of C-reactive protein from Channa punctatus (Bloch).

C-reactive protein (CRP) is found to be a normal component of serum of freshwater air-breathing murrel Channa punctatus. Based on the property of binding with C-polysaccharide (CPS) of pneumococcus bacteria in presence of Ca2+, CRP was purified by phosphorylcholine-Sepharose affinity column chromatography. Molecular weight of the intact protein molecule was estimated to be approximately 141,000 by gel filtration. In non-reduced and reduced conditions the molecule showed molecular weight approximately 28,000 and 14,000 respectively in SDS-PAGE. Monospecific antisera was raised against the affinity purified CRP and used as a tool to detect CRP in the hepatic cytosol and egg extract. The level of CRP in the normal serum was estimated to be 220 micrograms/ml.

Animals↗
PubMed1992

Responsiveness to gonadotropin releasing hormone of human term trophoblast cells in vitro: induction by estradiol.

Human trophoblast cells isolated from term placentae by enzymatic treatment and maintained in culture, spontaneously secrete hCG after 72hrs but fail to increase their gonadotropin secretion in response to exogenous GnRH. Treatment with estradiol for 24 hrs prior to challenge with GnRH induces the responsiveness to GnRH stimulation. This action of estradiol on trophoblast cells in independent of their stage of differentiation. The competitive displacement analysis of [125I] GnRH binding to estradiol-primed trophoblast cells indicated a single class of low affinity sites with Ka of 1.6 x 10(6) M-1, similar to that observed previously in placental membranes. To our knowledge this is the first demonstration of induction of GnRH receptors by estradiol in human trophoblast cells.

Binding, Competitive↗
PubMed1992

Monoclonal antibody to Japanese encephalitis virus cross-reacting with histones present in the cell nuclei.

An immunoglobulin G (IgG2b) class of monoclonal antibody (MoAb, NHA-1) raised against Japanese encephalitis virus (JEV) E glycoprotein, reacted with the viral antigen expressed in cytoplasm of the infected cells and also with the cell nuclei, by an indirect fluorescent antibody technique (FA). The NHA-1 reactivity to nuclei was found to be due to its recognizing a JEV cross-reactive epitope present on the nuclear histones. Adsorption with calf thymus histones (type II-AS) showed a drop in NHA-1 reactivity to both JEV and histones by an enzyme-linked immunosorbent assay (ELISA) and indirect FA; the drop was higher against the histones. The MoAb recognized specifically the viral antigens expressed on the infected porcine kidney cell surface by a modified indirect FA. ELISA carried out with glutaraldehyde-fixed antigens showed an almost 2-fold increase in the reactivity over unfixed JEV antigen but none for the histones. Thus, the results indicate that histones share a sequential homology with E glycoprotein of JEV, which might lead to an autoimmune disorder induced due to the molecular mimicry between these two antigens.

Animals↗
PubMed1992

Organization of repeated sequences in the region downstream to rRNA genes in the rDNA episome of Entamoeba histolytica.

The E. histolytica rDNA episome consists of a 3.7 kb HindIII fragment located downstream of the rDNA inverted repeats. We have determined the complete nucleotide sequence of this fragment and have shown that it is comprised of two families of short tandem repeats, the 170 bp DraI repeat and the 144 bp ScaI repeat. Each DraI repeat unit consists of 12-mer sequences with near complete homology to yeast consensus autonomously replicating sequence. In addition, a 21-mer subrepeat structure is also present in each unit. The sequence of the ScaI repeat is about 90% homologous with the sequence of another family of 145 bp tandem repeats, the PvuI repeats reported to be present upstream of the rRNA genes (3). Compared with most other parts of the rDNA episome, the downstream region showed frequent restriction fragment length polymorphism. This was due to changes in the number of tandem DraI repeat units. The loss of these repeats might explain how rDNA length heterogeneity observed in the clones of HM1:IMSS could have arisen. On the other hand, the number of ScaI repeat units in these clones remained unchanged. The repeat units found in the 3.7 kb HindIII fragment show superficial resemblance to equivalent regions of the intergenic spacers of higher eukaryotes. Moreover, these repeated sequences seem to be specific for the pathogenic strain of E. histolytica.

Animals↗
PubMed1992

Thyrotoxicity of the chlorides of cadmium and mercury in rabbit.

Exposure to heavy metals such as cadmium and mercury is of immediate environmental concern. The present study was aimed at establishing a direct relationship between heavy metal poisoning and thyroid dysfunction. Cadmium and mercury treatment at LD50 levels resulted in severe thyrotoxicosis in the rabbit. Within 24 h of intramuscular administration of cadmium chloride 15 mg.kg-1 body weight (bw) and mercury chloride 20 mg.kg-1 bw, thyroid peroxidase activity increased significantly over the control with a concomitant rise in the triiodothyronine (T3) titre. On the other hand, there was a remarkable fall in the thyroxine (T4) level, and the T3/T4 ratio was high as compared with the control. Evidence indicates that acute heavy metal lethality will induce immediate hyperthyroidism. It is suggested that T3-toxicosis may be produced by a preferential synthesis of T3 and/or preferential deiodination of T4 to T3. Measurement of T3 and T4 levels may thus be utilized as a reliable indicator of heavy metal lethality.

Animals↗
PubMed1992

Elevation of C-reactive protein in serum of Channa punctatus as an indicator of water pollution.

Effect of some pollutants like heavy metals, non-metals and pesticides on the circulating level of C-reactive protein (CRP) which is an acute phase plasma protein was studied in a freshwater murrel C. punctatus. Fish was exposed to nonlethal doses of these xenobiotics which were apparently safe. But the level of CRP detected by sensitive single radial immunodiffusion (SRID) technique showed that within 12 hr of exposure the nonlethal doses of xenobiotics could initiate the acute phase response in terms of elevated CRP titre. Heavy metals caused the acute phase within 24 hr, nonmetals and Metacid-50 within 48 hr exposure. The carbamate compound, carbaryl demonstrated a biphasic response to CRP level which may be correlated with the reversible type of anticholinesterase property of this compound while Metacid-50 is an irreversible type of anticholinesterase agent. The assessment of the CRP level in the serum of fish may be utilized as a primary bioindicator of a contaminated environment toxic enough to mount an acute phase response.

Ammonia↗
PubMed1992

Time dependent tissue distribution of 203Hg in the white rat and Anabas testudineus, a freshwater teleost.

The distribution of mercury, an environmentally important toxicant, has been evaluated in a time dependent manner in different tissues of white rat and a freshwater teleost, Anabas testudineus. Sampling was performed at 15 min, 2, 6 and 48 h post injection (im) of 203Hg mercuric nitrate. Radioactivity of the 5% tissue homogenate, serum and bile was measured in a Gamma Counter. The rate of 203Hg accumulation is higher in fish immediately after administration which, however, is more or less of equal rate in the later period of observation in both the experimental animals. Partitioning of 203Hg occurs in a species specific manner with higher levels recorded in the brain and gonad of white rat. Spleen, liver and kidney, however, are the major tissues to accumulate mercury in both the species. The present study highlights that kidney is the target site of mercury retention with a higher kidney/liver ratio of mercury.

Animals↗
PubMed1992

Comparative efficacy of fluoroquinolones, aminoglycosides, ureidopenicillins & newer cephalosporins against Pseudomonas species.

A total of 74 strains of Pseudomonas aeruginosa and 18 strains of Ps. putrefaciens were tested for sensitivity to 14 different antimicrobial agents. Ps. aeruginosa were mostly sensitive to netilmicin (81%), piperacillin (78%), amikacin (73%), azlocillin (70%), ceftazidime (69%) and pefloxacin (65%). Only 66 per cent strains of Ps. putrefaciens were sensitive to netilmicin, ceftazidime and ceftriaxone. The MIC values of the different drugs for the sensitive strains were comparable with the results of susceptibility testing. The Ps. putrefaciens strains showed greater resistance than Ps. aeruginosa.

4-Quinolones↗
PubMed1992

In vivo and in vitro acetylcholinesterase inhibition by metacid-50 and carbaryl in Channa punctatus under natural field condition.

Channa punctatus (Bloch), cultivated under paddy-cum-fish culture programs, was exposed to non-lethal levels of 0.239 ppb of Metacid-50 and 3.73 ppm of Carbaryl for 7 days in the field. These insecticides effected significant inhibition of brain acetylcholinesterase (AChE) activity accompanied by a concurrent increase in acetylcholine (ACh). In vitro inhibition of brain acetylcholinesterase was also compared between unexposed and pesticide exposed fish. Data revealed that the time taken for 50% inhibition (t0.5) of the enzyme was less in the pesticide exposed fish as compared to that of the unexposed fish. This indicates a preformed effect of the test pesticides on fish brain AChE during the in vivo exposure while the in vitro effect is an additive inhibition of the enzyme caused by the circulating inhibitors reducing the t0.5. Acetylcholinesterase inhibition may thus be utilized to monitor pesticide pollution in agricultural fields.

Acetylcholine↗
PubMed1992

A submicroscopic translocation, t(4;10), responsible for recurrent Wolf-Hirschhorn syndrome identified by allele loss and fluorescent in situ hybridisation.

A 2 year old girl presented with developmental delay and subtle dysmorphic features suggestive of Wolf-Hirschhorn syndrome (WHS). High resolution chromosome analysis was normal in the child and both parents. Molecular analysis indicated that the child had not inherited a maternal allele of probes from 4p16, confirming the clinical diagnosis. Prenatal diagnosis in the next pregnancy showed that again the fetus had no maternal allele for probes mapping to 4p16. Fluorescent in situ hybridisation in the mother showed a submicroscopic translocation, t(4;10). A normal karyotype in a child with clinical features of WHS is an indication for further investigation.

Abnormalities, Multiple↗
PubMed1992

Acetylcholinesterase characteristics of termite queen exposed to anticholinesterase compounds.

A regional profile of AChE activity was noted in the Indian termite queen Odontotermes redemanni with the head recording the higher and body the lower range of activity. The enzyme characteristics such as substrate and temperature optima were more or less similar while pH requirement for optimum AChE activity varied from 7.0 to 7.6 In vitro inhibition of head and body AChE was studied using pure and commercial anticholinesterase compounds. Interestingly, the commercial formulations like Metacid-50 and Carbaryl are potent enough at 1 x 10(-8) M to produce 50% in vitro inhibition of AChE of head and body regions within 15 min of preincubation. A 20 min of preincubation (t0.5) was necessary to record 50% in vitro inhibition of AChE with known and pure anticholinesterase compounds such as DFP (3.5 x 10(-10) M) and physostigmine (3.6 x 10(-10) M). It is surmised that (a) the response of the head and body AChE to the commercial formulations of the insecticides may be used as a reliable and sensitive bioindicator of pesticidal contamination of the terrestrial environment and (b) termite control may be successfully done with the application of organophosphate or carbamate compounds.

Acetylcholinesterase↗
PubMed1991

Clinical role of indium-111 antimyosin imaging.

Myocyte necrosis occurs in ischaemic, inflammatory and toxic heart diseases and can be detected by indium-111 antimyosin imaging. This allows a non-invasive evaluation of the site, extent and quantitation of the severity of myocardial necrosis. Simultaneous imaging of perfusion in patients with myocardial infarction allows the differentiation of necrosed and perfused areas and the varying degrees of mismatch and overlap, which has prognostic significance. 111In-antimyosin imaging is useful in the assessment of patients with unstable angina and in those for whom the diagnosis of infarction or unstable angina is not clear. In suspected myocarditis, a positive scan indicates the necessity for endomyocardial biopsy to confirm inflammation, whereas a negative scan makes the diagnosis of myocarditis unlikely. Antimyosin imaging is not useful as a marker of rejection in the 1 year post-transplant, but uptake after this period is associated with an increased rejection rate and is therefore an important tool in planning management strategies. Most patients on anthracycline treatment have demonstrable uptake, which is related to the cumulative dose and to the ejection fraction. Its role in this situation is as yet unclear. The use of new ligands and radioisotopes (99mTc) is likely to allow earlier imaging and produce improved quality.

Angina, Unstable↗
PubMed1991

111In antimyosin antibody uptake is related to the age of myocardial infarction.

The intensity of 111In antimyosin uptake is related to the type (Q wave or non-Q wave), size, and location of an acute myocardial infarction. To determine whether the uptake intensity is also related to the infarct age, and to establish the time frame during which 111In antimyosin uptake occurs postinfarction, we studied a quantitative measure of uptake intensity, the heart-lung (HL) ratio, in 90 consecutive patients at various intervals following a Q wave infarction. Imaging was performed 24 hours following 111In antimyosin injection. Uptake of antimyosin could be demonstrated up to 154 days postinfarction. The HL ratio was measured as the ratio of the maximum counts in the infarcted myocardium to the adjacent lung background, and was 1.93 +/- 0.51 (mean +/- SD) overall. The ratio declined exponentially with increasing age of infarct. Using nonlinear regression analysis, 47% of the total variance of the HL ratio could be attributed to age alone (p less than 0.001). Sequential antimyosin imaging was performed in nine patients, with the first study between 4 and 15 days, the second between 50 to 100 days, and the third between 125 to 270 days after the onset of chest pain. The HL ratios were (mean +/- SD) 1.77 +/- 0.25 for scans between 4 and 15 days, 1.54 +/- 0.12 for scans between 50 and 100 days (p less than 0.02), and 1.42 +/- 0.16 (p less than 0.05) for scans between 125 to 270 days, further establishing the progressive reduction in 111In antimyosin uptake with age of myocardial infarction.

Adult↗