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Biomedical subjects

S Benedict

Publications and source records attributed to S Benedict.

9 recordsLinked to original sources
PubMed1992

Degree of comfort in providing care to PWAs: effect of a workshop for baccalaureate nursing students.

A low-incidence area of HIV/AIDS was the site for this study, which examined baccalaureate nursing students' degree of comfort in providing basic nursing care to people with AIDS (PWAs), and determined the effect of a National Institutes of Health (NIH)-sponsored one-day workshop on HIV/AIDS on this degree of comfort. The pretest-posttest study revealed no statistically significant decrease after the workshop in the amount of discomfort associated with providing basic nursing care to PWAs. However, there were changes in the ranking of nursing procedures following the workshop. Included is a discussion of the workshop's effect on students' comfort level and implications for nurse educators. Ideas for related studies in the area of HIV health care are explored.

Acquired Immunodeficiency Syndrome↗
PubMed1991

Requirement of the adenovirus E1A transformation domain 1 for inhibition of PC12 cell neuronal differentiation.

Expression of the adenovirus early gene E1A inhibits the nerve growth factor (NGF)-induced differentiation of PC12 pheochromocytoma cells. Expression of the 12S form of E1A, which lacks the transcription activation region, also inhibited PC12 cell differentiation in a manner similar to the wild-type gene. Three cellular proteins--the retinoblastoma susceptibility gene product referred to as 105(Rb)-, 107-, and 300-kDa proteins--stably interacted with the different E1A polypeptides. Analysis of the association of these cellular proteins with mutant E1A polypeptides demonstrated that a functional domain 1, which is minimally involved in the association of the 300-kDa protein with E1A, was sufficient to inhibit neuronal differentiation. Deletion of transformation domain 2, which encodes sequences necessary for the binding of the 105(Rb)- and 107-kDa proteins, did not influence the ability of the mutant E1A polypeptide to inhibit PC12 cell differentiation. E1A was also shown to alter the expression of mRNAs for the early response genes c-fos, c-myc, egr-1, and c-jun and their regulation in response to NGF. In clones expressing either 12S or 13S E1A, NGF stimulation of c-fos and c-myc was repressed. In contrast, basal mRNA levels for c-jun and egr-1 were constitutively elevated and not significantly affected further by challenge with NGF. Simply expressing c-jun by gene transfer, however, did not mimic the action of E1A because constitutively expressing c-jun clones differentiated in response to NGF. Thus, expression of the E1A polypeptide disrupts NGF control of early transcription events that have been shown to be critical for PC12 cell neuronal differentiation.

Adenovirus Early Proteins↗
PubMed1989

The suffering associated with lung cancer.

The purpose of this study was to investigate the physical, psychological, and interactional aspects of lung cancer associated with suffering. A sample of 30 adults with primary pulmonary malignancies was obtained from an oncology clinic and from the practice of a thoracic surgeon. The subjects had been treated with chemotherapy, surgery, and/or radiation. A structured interview was conducted with each subject to determine the incidence of suffering associated with lung cancer. A five-point Likert-type scale was used to quantify the subjects' responses. The highest level on the scale, "Very Much" suffering, was reported to be associated with lung cancer by 50% of the sample. Ten percent of the sample reported no suffering. The following were reported to be the sources of greatest suffering: disability, pain, anxiety, changed daily activities, and weakness/fatigue. There were no statistically significant differences beyond the 0.05 level in reported suffering among groups treated with surgery, chemotherapy, or radiation. There was a statistically significant difference (p = 0.028) between groups with known metastatic disease and no known metastatic disease in the amount of suffering associated with the psychological aspects.

Adult↗
PubMed1988

Activation of Na+/H+ exchange and the expression of cellular proto-oncogenes in mitogen- and phorbol ester-treated lymphocytes.

It has been suggested that an intracellular alkalinization, resulting from stimulation of Na+/H+ exchange, is a necessary step and perhaps the signal leading to cellular proliferation in cells stimulated by mitogens. This hypothesis was tested by measuring the early stages of the proliferative cascade in cells where antiport activity was precluded by omission of Na+ or by the addition of potent amiloride analogs. To circumvent possible nonspecific effects due to long incubations under these conditions, an early response to mitogens, the increased level of c-fos mRNA, was monitored. In rat thymic lymphocytes, the increase in the level of c-fos RNA induced by the combination of 12-O-tetradecanoylphorbol 13-acetate and ionomycin was unaffected by inhibition of the antiport with 5-(N-ethyl-N-propyl)amiloride. Increased c-fos RNA was also observed in the absence of Na+ and when alkalinization was prevented by means of nigericin. Similar results were obtained with phytohemagglutinin-stimulated human T lymphocytes. Moreover, although the lectin stimulated the antiport in these cells, an alkalinization was not observed, due to the concomitant occurrence of an acidifying process. It was concluded that the stimulation of the Na+/H+ antiport that accompanies the addition of mitogens is neither sufficient nor necessary for the initiation of cellular proliferation.

Amiloride↗
PubMed1985

Isolation of three new avian sarcoma viruses: ASV 9, ASV 17, and ASV 25.

The newly isolated avian sarcoma viruses, ASV 9, 17, and 25, cause fibrosarcomas in young chickens and induce foci of transformed cells in chick embryo fibroblast cultures. They are defective in replication and belong to envelope subgroup A. The sizes of their genomes are 6 kb (ASV 9), 5 kb (ASV 17), and 6 kb (ASV 25), respectively. All three contain long terminal repeat (LTR) and gag sequences but lack pol. env is absent from ASV 9 and ASV 25, but some env sequences are detectable in ASV 17. None of the defective viral genomes hybridized to selected onc probes representing src, fps, yes, myc, myb, and erb A. erb B appears absent from ASV 9 and ASV 17, but some hybridization between the erb B probe and the RNA of ASV 25 was detected. ASV 9 codes for a transformation-specific gag-linked protein of 130kDa. Multiple gag-linked transformation-specific proteins are seen in ASV 17 and 25; they require further study.

Animals↗
PubMedDate not supplied

Nursing research priorities related to HIV/AIDS.

To determine the nursing research priorities related to HIV/AIDS, a two-round Delphi technique survey was conducted. Registered nurses in clinical, administrative, academic, and research nursing positions identified a total of 208 topics. The highest-ranked items were patient-centered and lower-ranked items were provider-centered. Subjects in clinical practice identified the nutritional aspects of AIDS care and hospice care as being of higher priority than did subjects in administrative, academic, and research positions. Complementary therapies and healthcare providers' fears were ranked as higher priorities by the administrative, academic, and research nurses than by the clinical practice subjects.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

RN education flexibility utilizing laptop computers.

In a geographic region that is largely rural, baccalaureate faculty teaching RNs needed a program of study that provided flexibility. This article describes a project that incorporates CAI and PCs into RN education. Difficulties and successes of the project are also discussed.

Alabama↗
PubMedDate not supplied

Desktop publishing.

Explore the source record for details and available documents.

Alabama↗