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Biomedical subjects

S Beck

Publications and source records attributed to S Beck.

At least 217 records · Page 12Linked to original sources
PubMed1983

Rat pancreas allotransplantation: a short term comparison of rejection patterns with different methods of exocrine drainage.

Pancreas transplantation has been performed in the rat to study the effect on rejection of duct ligation, duct obliteration and duct open intraperitoneal drainage. On day six, an acceleration of graft damage was seen in the duct ligated and obliterated allografts compared to the duct open group. However, by day 11, rejection was complete in all three groups. In comparison with pancreatic isografts, the histological diagnosis of rejection can be difficult to define, particularly in the duct ligated and obliterated groups. The most useful criterion was the localisation of the cellular infiltrate to the walls of small veins and within the islet of Langerhans.

Animals↗
PubMed1983

Isolation of Z-DNA-containing plasmids.

A purified, monoclonal antibody, specific for the left-handed Z-form of poly(dG-dC), was coupled covalently to Sephacryl S-1000 beads. Such an antibody column provides a convenient method to isolate and purify those plasmid DNAs that contain Z-DNA from a large excess of other DNAs, RNA, etc. From a library of Escherichia coli DNA, cloned into the vector plasmid pUC-8, several recombinant plasmids were isolated, which bind to this antibody. Thus, E. coli contains sequences, which in "natural" negatively supercoiled DNA, adopt a left-handed Z-DNA-like conformation.

Animals↗
PubMed1983

Pancreatic transplantation in the rat. Long-term study following different methods of management of exocrine drainage.

A major problem in pancreas transplantation is the management of exocrine drainage. Isotransplantation has been performed in streptozotocin-induced diabetic rats using a microsurgical technique, and four methods of exocrine drainage have been compared over a nine-month period. The duct system was ligated, left open to drain into the peritoneal cavity, or obliterated with latex or with Ethibloc I occlusion gel. Biochemical follow-up included determinations of serum glucose concentration, intravenous and oral glucose tolerance tests (GTTs), and insulin assays. Histological studies were performed at 1 and 9 months posttransplant. All animals became normoglycemic after the operation. Although 25 out of 28 remained normoglycemic throughout the period of study, oral GTTs performed at six months indicated impaired endocrine function in some of the ligated, freely draining, and Ethibloc I-obliterated animals. Latex-obliterated grafts showed normal oral GTTs up to 9 months postoperatively. Intravenous GTTs showed impairment of endocrine function in all groups, but this was least evident in the latex-obliterated rats. These changes were supported by the peak serum insulin levels during the GTTs. Histologically, long-term ligated, freely draining, and Ethibloc I-obliterated pancreas grafts showed similar degrees of exocrine degeneration, fibrosis, and disruption of islets of Langerhans. In contrast, long term latex-obliterated pancreas grafts demonstrated minimal exocrine tissue and intact islets.

Animals↗
PubMed1983

Cold storage of the pancreas with a view to preservation of islet cell function following transplantation.

Three solutions, hyperosmolar citrate, modified Collins' C2, and Sacks' II solutions were compared as media for cold storage preservation (arterial infusion and subsequent cold storage in the same medium at 0-4 C) of the rat pancreas with a view to preservation of endocrine function. Pancreatic isotransplantation was performed following cold ischemic intervals of 0, 24, 30, and 36 hr, into streptozotocin-induced diabetic recipients. Results were assessed by normoglycemic survival and insulin response, together with K values following i.v. glucose tolerance tests at 3 months postoperatively; 24-hr preservation was achieved with equal success using modified Collins' C2 solution or hyperosmolar citrate-but not with Sacks' II solution. Preservation for 30 hr was consistently successful using modified Collins C2 solution only, but the period could not be extended with success to 36 hr. Hypoglycemia and hyperinsulinemia occurred 24 hr postoperatively in the majority of animals receiving grafts stored in Sacks' II solution, but to a much lesser extent using modified Collins' C2 and hyperosmolar citrate. This was also temporarily seen in grafts stored for 36 hr in modified Collins C2 solution. At 3 months postoperatively after 30 hr cold ischemia, i.v. glucose tolerance tests showed the hyperosmolar citrate cold-stored grafts had lower K values and significantly reduced insulin responses compared with grafts stored in modified Collins' C2 solution. The modified Collins' C2 solution proved to be the most effective of the three solutions tested.

Animals↗
PubMed1983

Influence of the genotype of mice on the effect of interferon on phagocytic activity of macrophages.

The influence of genotype on interferon effect on phagocytic activity of unstimulated mouse peritoneal macrophages (MPM) was studied in in vitro experiments. Treatment of MPM from BALB/c and ICR mice with mouse fibroblast interferon (MuIFN-beta) enhanced the ingestion of non-opsonized Escherichia coli. This effect was dose-dependent and neutralized by anti-interferon globulin. MPM from C57B1/6 mice were not stimulated by the same treatment. Treatment of MPM with pH 2-sensitive immune interferon (MuIFN-gamma) depressed the ingestion independently on the genotype of mice.

Animals↗
PubMed1981

The effects of targeting improvements in urine glucose on metabolic control in children with insulin dependent diabetes.

A treatment program designed to increase the percentage of negative urine tests was implemented using a multiple-baseline across groups design in a sample of 19 families of children with insulin dependent diabetes. The treatment involved instruction in insulin adjustment, decrease in intake of simple sugars and saturated fats, and increase in exercise, along with teaching the parents to support improvements in children's self-regulatory behaviors using a point economy and praise. New procedures designed to measure and reinforce adherence to the urine testing regimen were developed. Results showed significant increases in percentage of negative urines consistent with implementation of treatment across the three treatment groups, which were maintained over the follow-up period. Metabolic measures of control, including glycosylated hemoglobin and serum glucose did not show improvements even though the relationship between the percentage of negative urine tests and glycosylated hemoglobin was very high during treatment.

Behavior Therapy↗
PubMed1978

A stereological analysis of the effect of 19-nortestosterone (Durabolin) on rat skeletal muscle.

It is known that some anabolic steroids are capable of increasing the sizes of skeletal muscles or increasing their mechanical performance. The present study was undertaken to establish quantitatively the alterations which occur in skeletal muscle fibres as a result of the injection of 19-nortestosterone (nandrolone phenyl proprionate). Specimens of diaphragm were obtained from the experimental and control animals and prepared for electron microscopy and stereological analyses. Following low doses of the steroid stereological analyses have shown that in Type I and in intermediate fibres their mitochondrial volume proportions increase though their myofilament contents decrease. With higher doses, stereological analyses have shown that the mitochondrial volume proportions were increased particularly in intermediate fibres whilst the myofilament content of Type I and II fibres was significantly reduced.

Animals↗
PubMed1976

Psychiatric training for emergency medicine residents on a multidisciplinary team.

The Cincinnati General Hospital Emergency Department has a training program for emergency medicine residents on a multidisciplinary emergency psychiatry team. This essential training should occur in the emergency department setting rather than in psychiatric inpatient units of state hospital settings. There are advantages and disadvantages to this arrangement. Nonmedical members of the emergency psychiatry team train and support emergency medicine residents in a multidisciplinary approach to treatment. Some observations are made about how the emergency medicine residents deal with emotionally disturbed patients. Finally, 80% of emergency medicine residents responded to questionnaire on their reactions to the multidisciplinary emergency psychiatry team.

Attitude of Health Personnel↗
PubMed1976

Levels of alpha-fetoprotein in amniotic fluids of mice (curly-tail) with neural tube defects.

Mutant curly-tail mice are genetically predisposed to produce offspring with neural tube defects. Estimation of alpha-fetoprotein in the amniotic fluid of fetuses of these mutants has shown that the levels are raised in fetuses with exencephaly and open spina bifida. This suggests that these mice are a valid model for studies of the aetiology and genesis of neural tube defects in man.

Amniotic Fluid↗
PubMed1975

Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.

A rapid quantitative assay of phagocytosis has been developed. The method has been used to determine the phagocytic capability of human or dog polymorphonuclear leukocytes (PMN's) in a defined medium without added sera. The engulfment of 3H-labeled Staphylococcus albus is essentially complete in 30 minutes at 37 degrees C. Increase of phagocyte radioactivity is directly proportional to PMN per cubic centimeter over a wide range of cell concentrations. Maximal phagocytosis is observed at pH 7.5.

Animals↗