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Biomedical subjects

S Beck

Publications and source records attributed to S Beck.

At least 181 records · Page 10Linked to original sources
PubMed1990

Benign gastric ulcers and pernicious anemia.

The formation of a benign gastric ulcer in an achlorhydric milieu is a rare phenomenon. Since 1971, only 12 cases have been reported. Early cases were treated surgically for fear of gastric cancer, but since that time such ulcers have been successfully treated medically. The authors describe the case of a patient who had no concomitant ulcerogenic factors and whose ulcer occurred in the gastric cardia at the esophagogastric junction. We believe this is the first case report of a patient with pernicious anemia meeting these conditions.

Achlorhydria↗
PubMed1990

A new filter technique permitting traditional histopathological assessment of fine needle aspiration specimens.

We describe a new filtration method based on a transport medium technique which allows histopathological assessment of the tissue fragments present in many fine needle aspirates. Fine needle aspiration cytology and biopsy preparations obtained from equally divided transport medium specimens were compared in a study comprising 104 palpable breast lesions and 29 other miscellaneous lesions. In general, fine needle aspiration biopsy provided better information on tumour origin and differentiation than fine needle aspiration cytology, particularly with systemic aspirates and those which were heavily bloodstained. In most instances fine needle aspiration biopsy also provided sufficient material to perform specialised staining and immunohistological techniques, and it is suggested that it may have a role in supplementing direct smear fine needle aspiration cytology in these areas.

Biopsy, Needle↗
PubMed1989

Chemiluminescent detection of DNA: application for DNA sequencing and hybridization.

A non-radioactive DNA detection chemistry is described and its application is shown for DNA hybridization and standard dideoxy DNA sequencing. The method employes a biotin-streptavidin system which binds an enzyme specifically to a target DNA and upon exposure to substrate, the enzyme catalyzes a chemiluminescent reaction. The image is captured within seconds by a Polaroid or X-ray film. The method is capable of detecting DNA in the hundred attomol range.

Base Sequence↗
PubMed1989

Patients' perceived service needs when seen in a psychiatric emergency room.

Patients who came to a psychiatric emergency room and were assessed as not needing psychiatric hospitalization were interviewed regarding their service needs for the month prior to their index visit. The highest expressed needs were for information and advice, financial assistance, counseling and assistance in controlling emotions, while leisure-time activities, meeting people, budgeting, medications, and getting along with others were the lowest areas of expressed need. Those who sought help generally went to appropriate community resources rather than informal supports. For some, coming to the psychiatric emergency room was their way of addressing their needs. For a number, their methods of coping were to escape from their problems through the use of drugs and alcohol or sleep. Implications for programming in the psychiatric emergency room are discussed.

Adolescent↗
PubMed1988

Protein blotting with direct blotting electrophoresis.

Direct blotting electrophoresis, a method designed to be of general application for the separation and electroblotting of macromolecules, has been adapted to produce protein blots suitable for subsequent processing by standard techniques such as dye staining or immunological detection. After their separation in a very short gel the protein bands are electrophoresed out of the gel onto an immobilizing matrix. The matrix which is moved across the bottom of the gel by a conveyor belt binds these proteins with high affinity. Once the protein samples have been loaded onto the gel and electrophoresis has been started, no further intervention is needed until the blot is completed. The total expenditure of time for such a direct blot is less than 4 h for a mixture of proteins in the molecular weight range of 14-70 kDa. The staining sensitivity of directly blotted proteins is about 200 ng protein per band as revealed by India ink staining.

Collodion↗
PubMed1988

Human cytomegalovirus encodes a glycoprotein homologous to MHC class-I antigens.

Primary infection with human cytomegalovirus (HCMV) is persistent and widespread, with symptoms that are mostly subclinical but can cause serious illness or death, particularly in immunosuppressed patients. Recently, proteins from HCMV were shown to bind beta 2-microglobulin (beta 2-m) a protein that is normally found associated with the class-I major histocompatibility complex (MHC) antigens, which are essential for self-non-self recognition in the immune response. These findings led to the proposal that the virus may use beta 2-m binding as an infection mechanism. Here we present evidence from DNA sequence analysis that HCMV encodes a molecule similar to the MHC class-I antigens of higher eucaryotes, and propose that this protein is responsible for the observed beta 2-m binding. The deduced amino-acid sequence of the HCMV class-I-like protein reveals conservation of typical features of class-I structure, but we predict that the gene is not spliced, in contrast to the cellular genes.

Amino Acid Sequence↗
PubMed1988

Family members' perspectives on psychiatric hospitalization and discharge.

Families of currently hospitalized patients in an extended-care psychiatric facility were interviewed concerning their views of hospitalization and discharge. Although most were sad that their relative was hospitalized, only a third felt that they would rather be caring for their relative at home. Families felt communication with hospital staff was poor and many of their needs were unmet. Families indicated a number of concerns around discharge. If clients were to be discharged soon, most families felt the clients needed a highly structured supervised living arrangement.

Adult↗
PubMed1987

Colorimetric-detected DNA sequencing.

A sensitive, colorimetric method for visualizing the band pattern of DNA sequencing reaction is described. The enzymatic incorporation of radioactive nucleotides commonly used for the band detection is replaced by biotin conjugated to the 5'-terminus of a synthetic oligonucleotide by chemical synthesis. The oligonucleotide so labeled is used as a primer for dideoxy DNA sequencing in a primer extension reaction. The products of the sequencing reactions are analyzed on a denaturing polyacrylamide gel using the direct blotting electrophoresis technique. This technique makes it possible to transfer the band pattern during the electrophoresis onto an immobilizing matrix, on which it is made visible by an enzymatic reaction in less than 3 h. This biotin-based detection method is so sensitive that the sequencing reactions can be performed under the same conditions and concentrations as those for the radioactive detection.

Bacterial Proteins↗
PubMed1987

Cortical and vascular prostaglandin synthesis during renal allograft rejection in the rat.

Alterations in local prostacyclin and thromboxane synthesis could mediate the changes in vascular perfusion and platelet deposition in acutely rejecting renal allografts and prostaglandin E2 (PGE2) has been implicated in the regulation of the immune response. 6-Keto-prostaglandin F1 alpha (6 KetoPGF1 alpha), thromboxane B2 (TxB2) (the stable degradation products of prostacyclin and thromboxane A2 [TxA2], respectively) and PGE2 were measured in incubates of cortical slices taken from rat renal allografts or isografts one to seven days after transplantation. 6 KetoPGF1 alpha and TxB2 synthesis was also measured in incubates of blood vessels supplying and transplanted with the kidney in these animals. During the phase of cellular rejection (3-5 days), TxB2 synthesis was selectively elevated in allografted renal cortex, renal artery, renal vein, and abdominal aorta in comparison with isografted tissues. There was also a small but significant rise in cortical PGE2 synthesis at this time, but vascular and cortical 6 KetoPGF1 alpha production remained unchanged. Renal infarction, occurring 7 days after transplantation, was accompanied by a nonspecific rise in the synthesis of all three prostaglandins by renal cortical slices. Increased tissue TxA2 synthesis may contribute to local thrombosis and decreased graft perfusion during acute rejection, thereby potentiating graft destruction.

6-Ketoprostaglandin F1 alpha↗
PubMed1987

Can histopathologists reliably assess dysplasia in chronic inflammatory bowel disease?

A copy of the standardised classification (SC) proposed for assessing dysplasia in inflammatory bowel disease was circulated to six histopathologists who were asked to apply it to 40 slides from 34 patients with ulcerative colitis to test its reproducibility. The slides were relabelled and recirculated to the pathologists at least one month later. Each was asked to state whether or not key diagnostic features were present before giving a final dysplasia score for the second assessment. Only minor interobserver and intraobserver disagreements were recorded. Pathologists were most consistent at recognising back to back glands, villous mucosal architecture, hyperchromatic nuclei, stratification of nuclei, regenerative nuclei and loss of nuclear polarity. There was poor interobserver agreement in assessing dystrophic goblet cells and columnar mucous cells. Back to back glands, hyperchromatic nuclei, loss of nuclear polarity, stratification of nuclei and columnar mucous cells were considered to be the most important features for determining the severity of dysplasia. As there was poor interobserver agreement in assessing columnar mucous cells and dystrophic goblet cells these features need to be more clearly defined or should be removed from the SC.

Adult↗
PubMed1986

Pharmacokinetics of ofloxacin and theophylline alone and in combination.

The pharmacokinetic interactions of ofloxacin (2 X 200 mg) and theophylline (3 X 200 mg) were investigated in 12 healthy volunteers over a period of two weeks. In the first week, theophylline was given over five days to reach a steady state. In the second week, the combination of theophylline and ofloxacin was applied. Cmax, tmax, AUC0-8, the serum elimination constant and serum half-life of theophylline were not changed when theophylline was given alone or in combination with ofloxacin. The kinetic parameters of ofloxacin were in accordance with data from the literature.

Adolescent↗