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Biomedical subjects

S Bazin

Publications and source records attributed to S Bazin.

At least 55 records · Page 3Linked to original sources

[Cathepsin D and the catabolic degradation of proteoglycans in granulomatous tissue].

Incubated in vitro at 37 degrees C, granulation tissues release glycosaminoglycans into the incubation medium. Such release is inhibited if pepstatine is present in the medium. From this result, it can be inferred that the protien moiety of the proteioglycans is degraded by cathepsin D. Therefore a role of this enzyme in granulation tissues appears, especially in the late reparative phase.

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Observations on the staining of ceruloplasmin following disc-electrophoresis utilizing polyacrylamide gels.

In our hands the dianisidine technique for the staining of ceruloplasmin in polyacrylamide gels following disc electrophoresis has proven unsatisfactory for three reasons. First, staining of ceruloplasmin could not be achieved with physiological amounts of the glycoglobulin present in serum. Second, even when relatively massive quantities of pure ceruloplasmin permitted effective staining there was loss of stain intensity with increasing electrophoresis time. Third, the necessity of utilizing dianisidine in alcoholic solution produces undesirable shrinkage and distortion of the gels. However, a modification of the p-phenylenediamine technique for the staining of ceruloplasmin in agar gels has been found to stain ceruloplasmin very effectively in polyacrylamide gels following disc electrophoresis.

Animals↗