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Biomedical subjects

S Basu

Publications and source records attributed to S Basu.

At least 487 records · Page 27Linked to original sources
PubMed1975

Sialic acids. Enzymatic synthesis of Tay-Sachs ganglioside.

A particulate preparation from embryonic chicken brain catalyzed the transfer of N-acetylgalactosamine from uridine diphospho-N-acetylgalactosamine to the ganglioside GM3 (hematoside, sialyllactosylceramide). The kinetic properties of the transferase were determined. The product was isolated and on the basis of chemical analysis and chromatographic behavior was shown to be Tay-Sachs ganglioside (GM2). The particulate preparation also utilized N-acetyl-D-glucosamine and some of its derivatives as acceptors, but partial heat inactivation and substrate competition experiments indicated that the two classes of acceptors, hematoside and N-acetylglucosamine, were substrates for different N-acetylgalactosaminyltransferases. The enzyme that utilized hematoside showed low but detectable activity with analogues such as lactosylceramide and sialyllactose, but no activity with a wide range of other beta-galactosides and glycosphingolipids. These results are in accord with a proposed pathway for the biosynthesis of the gangliosides and for the patterns of these substances in different cell types and tissues.

Animals↗
PubMed1975

Enzymatic synthesis of a blood group H-related glycosphingolipid by an alpha-fucosyltransferase from bovine spleen.

An alpha-fucosyltransferase activity has been detected in a purified membrane preparation isolated from bovine spleen which catalyzes the transfer of L-fucose from GDP-L-[14C]-fucose to a tetraglycosylceramide (Lac-nTet-cer, Galbetal-4GlcNAcbeta1-3Galbeta1-4-Glc-cer) to form the blood group H-related glycosphingolipid. The membrane preparation contained a highly active endogenous nonlipid acceptor, which could be precipitated by 5% trichloroacetic acid or chloroform-methanol-water (6:3:5, v/v/v), whereas there was little endogenous glycosphingolipid acceptor. The optimum pH value for the incorporation of L-fucose was 6.4 in cacodylate-HCl buffer. The Km values were 0.6 mM and 0.36 mM for Lac-nTet-cer and GDP-L-fucose, respectively. The 14C-labeled product of the reaction was isolated and purified; it migrated with human erythrocyte blood group H-active pentaglycosylceramide. the terminal [14C]fucose was hydrolyzed 85% and 55% by 0.1 N trichloroacetic acid at 100 degrees for 2 hours and Charonia lampas alpha-fucosidase (19 hours at 37 degrees), respectively. The 14C-labeled product inhibited the hemagglutination reaction of O-type cells against eel anti H(O) globulin and formed a precipitin line with Ulex europeus lectin.

Animals↗
PubMed1975

Prevalence of Salmonella serotypes in India: a 61-year study.

During the period 1958-73, 8 027 strains of Salmonella were tested at the National Salmonella and Escherichia Centre: 3 834 strains from man, 3 018 from animals, 839 from sewage and water sources, and 336 of unknown origin. A total of 99 serotypes were identified: 47 from man, 83 from animals, and 35 from sewage and water sources. S. typhi was the commonest serotype in man, followed by S. weltevreden and S. paratyphi A. S. typhimurium was the commonest serotype isolated from animals, followed by S. weltevreden and S. anatum. In sewage and other water sources S. weltevreden was the commonest serotype identified followed by S. typhimurium and S. bareilly. Infection due to S. weltevreden in both man and animals increased considerably after 1970. Out of a total of 99 serotypes isolated from different sources, 13 were isolated from man only, 49 from animals only, 34 from both man and animals, 30 from both animals and water sources, and 22 from man, animals, and water sources.

India↗
PubMed1975

Salmonella weltevreden: a sero-type of increasing public health importance in India.

Infection due to Salmonalla weltevreden in man in India was quite uncommon until 1970, constituting less than 4% of total human salmonellosis. The incidence of infection increase considerably since that year and in 1972 this serotype constituted 29.1% of total human and 22.4% of both human and and animal salmonellosis. This serotype ranks second among 47 and 83 Salmonella serotypes isolated from sewage and other water sources during 1958-1973. The public health importance of this serotypes is discussed.

Animals↗