[Botulism in Puglia: bacteriologic research on 6 episodes occurring between 1977 and 1982].
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Biomedical subjects
Publications and source records attributed to S Barbuti.
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Some properties of 5 H2S+ strains of E. coli (labeled EC1-EC5, of which 4 isolated from the feces of subjects with gastroenteritis and 1 from a patients with cystopyelitis) has been studied. Of these, two resulted to belong to the O140 and one to the O127 serological groups; other 2 strains were found to react respectively with two (O84 and O108) and three (O65, O70 and O71) E. coli antisera. H2S- segregants were spontaneously obtained from 3 of these strans, but with different frequency: it was very low in EC5 (1.4%) and especially in EC3 (0.2%) strains, but appeared higher in EC4 (39.3%) strain. Clones from EC4 strain were also raffinose-negative; 11 of 12 obtained from EC5 strain (resistant to tetracycline) appeared sensible to tetracycline. Only one strain (EC4) has been able to transfer the H2S+ character with 4.7% frequency; another strain (EC5) transferred tetracycline-resistance character, but with lower frequency. Raffinose-positive character has never been transferred.
The significance of the presence of Mycoplasma pharyingis and Mycoplasma salivarium in patients with chronic respiratory disease has been evaluated by studying of specific antibody and aerobial bacterial flora. The isolation of Mycoplasma pneumoniae was unsuccessfull in 173 cases with chronic respiratory disease. Among them 76 were carriers of Mycoplasma pharyngis and Mycoplasma salivarium. Pathogen and opportunist aerobial bacteria were isolated as frequently in the cases under study as in the control group, where mycoplasma cultures were negative. Complement fixation and indirect hemagglutination tests for detection of specific antibodies were carried on 45 of the cases under study (19 Mycoplasma pharyngis and 26 Mycoplasma salivarium carriers). In the carriers of Mycoplasma pharyngis the frequency of complement fixing antibody antibodies was higher (36.8%) than in the others (19%). Neverthless, it seems unacceptable an etiological correlation between Mycoplasma pharyngis and chronic respiratory disease.
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Research of antibodies anti-N. meningitidis serogroups A, B, and C was carried out on 248 healthy children 7 months-10 years aged by indirect hemagglutination, to determine the age of their initial demonstration into the serum and the time of their clearness, besides the diffusion of antibodies within the infantile population. Antibodies against one or more serogroups of N. meningitidis were detected already in the second half-year life in 73 percent examined children. Frequence of antibodies is progressively increasing in the last years until to findings about 90-100%. Antibodies against N. meningitidis serogroups A were detected more frequently, and in a less degree, the ones against serogroup C. Research of antibodies after 2 and 12 months was repeated on 84 subjects; for the most part of these the antibody titre was the same.
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The production of coagulase, DNase and haemolysins by 30 L-forms obtained from as many strains of Staphylococcus aureus having these properties, has been studied using different techniques. All of L-strains produced coagulase, for the most part after incubation for 24 h, but only some produced DNase and haemolisins. At the same time, the sensitivities of various antibiotics of staphyloccal L-forms and the parent strains has been determined. All of L-forms, as regards to bacterial form from which they were derived, showed an high resistance to antibiotics of which the primary site of action is upon cell wall (penicillin, ampicillin, methicillin, cephalosporins, vancomycin), with the exception of bacitracin. The tetracycline was more effective against the L-forms, while the group that includes chloramphenicol, novobiocin, streptomycin, gentamycin and kanamycin inhibited coccal and L-forms about equally.
The production of enterotoxin by L-forms obtained from strains of Staphylococcus aureus (10 enterotoxin A-producing strains, 7 enterotoxin B, 2 enterotoxin C and 1 which produced both enterotoxins A and C) has been studied by agar-gel diffusion technique. Using a proper technique, enterotoxin could be detected for 4 L-forms; 3 of these produced the same enterotoxin as parent strain (2 enterotoxin A and 1 enterotoxin B). As regards the other L-form, obtained from strain of St. Aureus which produced both enterotoxins A and C, only enterotoxin A could be detected.
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