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Biomedical subjects

S B Phillips

Publications and source records attributed to S B Phillips.

26 records · Page 2Linked to original sources
PubMed1990

Involucrin-like proteins in non-primates.

A monoclonal and two polyclonal antibodies to human involucrin were used to look for involucrin epitopes in other species. All antibodies react strongly with the same proteins of monkey, and both polyclonal antibodies react with specific proteins of cow and dog. One of the polyclonal antibodies also reacts with proteins of sheep, guinea pig, rat, and finback whale. The immunoreactive proteins from cow and dog could be purified using a procedure developed for human involucrin. The reaction with the purified dog protein could be blocked by purified human involucrin. The results suggest that involucrin-like proteins have a wider species distribution than originally appreciated.

Animals↗
PubMed1987

A new class of soluble basic protein precursors of the cornified envelope of mammalian epidermis.

The cornified envelope has been shown to be formed beneath the plasma membrane as a result of the cross-linking of soluble and membrane-associated precursor proteins by transglutaminase. We have obtained a monoclonal antibody which reacts with the periphery of cells in the upper layers of human epidermis by indirect immunofluorescence (IIF) following immunization of mice with cornified envelopes of cultured human keratinocytes. The antibody also stained the cell peripheries of bovine, rat and mouse epidermis as well as stratified epithelium. Neutral buffer extracts of human cultured keratinocytes and epidermis examined under denaturing conditions contained polypeptides of molecular weight 14,900 and 16,800 which reacted with the antibody, and an additional component of molecular weight 24,800 was found in cultured cells. The polypeptides were shown to have a pI of about 9.0. Under non-denaturing conditions the two lower-molecular-weight polypeptides had an apparent molecular weight of 30,000, while the 24,800 protein had one of 60,000. Incubation of the polypeptides under conditions that activate transglutaminase resulted in a disappearance of the polypeptides or the formation of cross-linked products. Basic polypeptides with somewhat different pI values and molecular weights were identified in neutral buffer extracts of bovine and rat epidermis. The HCE-2 antibody appears to identify a new class of basic protein precursors of mammalian cornified envelope.

Animals↗
PubMed1987

Characterization of monoclonal antibodies generated to the cornified envelope of human cultured keratinocytes.

The cornified envelope of keratinocytes is an insoluble structure formed beneath the plasma membrane at the base of the stratum corneum. It is made by cross-linking precursor proteins by a membrane-associated transglutaminase. We have prepared monoclonal antibodies to the cornified envelope of cultured human keratinocytes and used these to identify precursor proteins using Western blotting. We have uncovered a number of precursors including involucrin and a 195 kD membrane-associated protein, which had previously been reported. Antibodies to these precursors, with the exception of the one to involucrin, reacted with the epidermis of other mammalian species, suggesting structural conservation in at least some envelope components.

Animals↗
PubMed1987

NM1 keratinocyte line is cytogenetically and biologically stable and exhibits a unique structural protein.

We have previously described a human keratinocyte line, NM1, which had been carried for more than 400 doublings, was trisomic for chromosome 8, and appeared to make a number of structural proteins characteristic of keratinocytes. This line has now been carried for more than 800 doublings and grows with the same vigor. It reaches confluence in 7 to 10 days and can be grown without a feeder layer for more than 15 passages. Its karyotype has remained 47,XY, +8. The current NM1 cells make readily detectable amounts of 67 kd and 48 kd keratins, and it has been established that the previously poorly resolved 58 kd band actually consists of 58 kd and 59 kd bands. We have also found that the apparent 56 kd band consists of the 56 kd and 56.5 kd bands. A unique basic polypeptide precursor of the cornified envelope has been discovered in the NM1 line. Although similar in charge to one in normal cells it is lower in molecular weight.

Carcinogens↗
PubMed1983

Radiology of postnatal skeletal development. VII. The scapula.

Twenty-four pairs of scapulae from fetal specimens and 35 pairs of scapulae from postnatal cadavers ranging in age from full-term neonates to 14 years, were studied morphologically and roentgenographically. Air-cartilage interfacing was used to demonstrate both the osseous and cartilaginous contours. When the entire chondro-osseous dimensions, rather than just the osseous dimensions, were measured, the scapula had a height-width ratio ranging from 1.36 to 1.52 (average 1.44) during most of fetal development. The exceptions were three stillborns with camptomelic, thanatophoric, and achondrogenic dwarfism in which the ratio averaged 0.6. At no time during fetal development was the glenoid cavity convex; it always had a concave articular surface. However, the osseous subchrondral countour was often flat or slightly convex. In the postnatal period the height-width ratio averaged 1.49. The ratio remained virtually unchanged throughout skeletal growth and maturation. In a patient with unilateral Sprengel's deformity the ratio for the normal side was 1.5, while the abnormal was 1.0. The cartilaginous glenoid cavity was always concave during postnatal development, even in the specimens with major structural deformities, although the subchondral osseous contour was usually flat or convex during the first few years of postnatal development. Ossification of the coracoid process began with the development of a primary center at three to four months. A bipolar physis was present between the primary coracoid center and the primary scapular center until late adolescence.

Acromion↗
PubMed1977

API computer profiles: correlation of API 20E with API 10S.

A comparison of identifications of 201 clinical isolates with the 21-test API 20 Enteric kit and the subset of tests in API 10S indicated 85.6% agreement at the species level and 93.5% agreement at the genus level.

Bacteriological Techniques↗
PubMed1976

MORLUC numeric system for the identification of Enterobacteriaceae.

Foul hundred eighty-six members of the Enterobacteriaceae representing nine genera were identified by conventional methods, and the results were compared with MORLUC (Biotrol Company Inc., Jamaica, N.Y.). MORLUC, an acronym for melibiose, ONPG (o-nitrophenyl-beta-galactopyranoside), rhamnose, lysine decarboxylase, urease, and citrate, are six prepackaged reagent-impregnated paper loops which are sealed within a plastic packet. The hydrogen sulfide reaction obtained from a triple sugar iron slant is coupled with MORLUC results and is readily converted into a three-digit numerical code, which is referenced on a preprinted single page listing. Additionally, the triple sugar iron is used to confirm the glucose fermentation by an unknown isolate. Comparisons of individual MORLUC tests and standard methods results in a better than 92% agreement, except for unrease. Four hundred sixty-six of the 486 bacterial isolates, or 96% of the strains which were numerically identified by MORLUC, agreed with conventional diagnoses.

Carboxy-Lyases↗