Biomedical subjects

S B Andersen

Publications and source records attributed to S B Andersen.

18 recordsLinked to original sources

PubMed2001

Geophysics. Longitudinal variation in springtime ozone trends.

Satellite measurements indicate that mid-latitude ozone depletion in the Northern Hemisphere spring during 1979-97 was worst over Europe and Russia. Here we show that these longitudinal differences in ozone trends are due to a combination of decadal variations in the circulation and transport of ozone-depleted air from the polar vortex. Any increase in ozone depletion in the polar vortex as a result of future cooling of the stratosphere would therefore be particularly bad over Europe and Russia.

Journal Article↗

PubMed2000

Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat.

Three types of callus tissues established from anther culture of eleven doubled haploid (DH) lines of wheat (Triticum aestivum L.) were evaluated for their ability in enhancing friable embryogenic (Type II) culture differentiation and genetic transformation. Differences between types of callus inocula were highly significant (P < 0.001), suggesting that the quality of the initial callus explant is of profound importance in encouraging the proliferation of Type II cultures. Other factors found to be crucial included weekly subculture of friable embryogenic callus tissues on a maintenance medium containing 30 microM dicamba and a predominance of amino-acid nitrogen supplement. Transfer and integration of the beta-glucuronidase gene was also affected by the type of inoculum when suitable embryogenic cell cultures were transformed using silicon carbide whiskers and high velocity microprojectiles. Expression of the hygromycin phosphotransferase selectable marker gene sequence was confirmed in all the stably transformed cell lines maintained on selection media containing lethal levels of hygromycin. Comparatively, there were differences in the frequency of regenerable, transgenic clonal segments between whisker-treated and microprojectile bombarded tissues mainly as a result of the fact that cultures vortexed with whiskers were more capable of post-treatment cell proliferation and embryo differentiation than those bombarded with cDNA-coated microprojectiles. Conditions for obtaining these results are outlined and discussed in relation to the suitability of the two transformation strategies for producing transgenic cell aggregates of wheat.

Cell Differentiation↗

PubMed1993

[Chlamydia pneumoniae].

Chlamydia are obligate intracellular bacteria with a biphasic life cycle. In 1986 a new species of Chlamydiae, Chlamydia psittaci TWAR was discovered, later named Chlamydia pneumoniae. C. pneumoniae is probably an important pathogen causing respiratory tract infections in man, ranging from subclinical infection to life threatening pneumonia. Infections can occur in epidemics. Furthermore, associations between C. pneumoniae infection and coronary artery disease, lethal myocarditis and sarcoidosis have been suggested. The current diagnostic method is based on detection of specific antibodies against C. pneumoniae in blood. Macrolides or tetracyclines are the drugs of choice for treatment of C. pneumoniae infection.

Chlamydia Infections↗

PubMed1991

[The polymerase chain reaction used in microbiologic diagnosis].

Among the new DNA technological methods for microbiological diagnosis, the most promising seems to be the polymerase chain reaction (PCR). The sensitivity of the method is approximately 100% and the specificity is between 90% and 100%, depending on the availability of an already existing test sufficient for comparison. The PCR is able to detect only one microorganism in clinical samples. Because of this high sensitivity, contamination of samples is one of the major problems. After certain preparation procedures, the method can be used on different kinds of human sample material. The PCR could be used for detection of a microorganism, for detection of a single gene (toxin gene) or for typing of microorganisms. It is expected that, in the immediate future PCR will be a prominent part of the methods used in microbiological diagnosis.

Bacterial Infections↗

PubMed1984

Lateral and anterior instability in acute ankle distortion. A radiologic investigation.

In a prospective consecutive investigation radiologic tests for lateral and anterior instability in acute ankle distortion were found to be reliable indications of lateral ligament ruptures. Arthrography of the ankle joint was used as reference. Absence of instability, however, gives little information as to the ligamental status. The size of this group constitutes a clinical problem. Better results are achieved by combining lateral and anterior instability tests. If only one test is used, the anterior instability test should be preferred. The results of the two tests bare no relation to the degree of ligament ruptures found at operation.

Adolescent↗

PubMed1982

Ruptures of the lateral ligaments of the ankle. Clinical diagnosis.

During the 11-month period 1 October 1977 to 31 August 1978 a total of 44 patients with acute supination trauma of the ankle were examined. The clinical findings were compared with the results of arthrography. This revealed that direct and indirect tenderness of the anterior talofibular ligament and calcaneofibular ligament respectively, combined with a greater than or equal to 4 cm swelling anteriorly and over the lateral malleolus, indicated a ligament injury with great likelihood. If some of the diagnostic signs are absent, most emphasis should be laid on the swelling over the lateral malleolus and on the direct and indirect tenderness of the calcaneofibular ligament. The talar-tilt test and examination for the drawer sign were of limited diagnostic value.

Adolescent↗

PubMed1981

Rupture of the lateral ligaments of the ankle: operation or plaster cast? A propective study.

The material comprises 444 patients, aged 12-50 years, with acute sprain of the ankle, seen over an 11-month period (1.10. 1977 to 31.8. 1978). Clinical examination showed no rupture of the ligaments in 53. Arthrography in the remaining patients revealed rupture of the lateral ligaments in 209 (in two-thirds of the anterior talofibular ligament and in one-third of the anterior talofibular as well as calcaneofibular ligament). Conservative treatment with a below-knee plaster cast for 5 weeks was employed in 107 patients, while 102 were treated surgically by suture of the ruptured ligament(s) and subsequently wore a below-knee plaster cast for 5 weeks. The follow-up 1 year after the accident was attended by 63 per cent of the patients. Good results were found in 76 per cent of those treated by plaster cast only and in 81 per cent of those treated by surgery. The difference is not statistically significant.

Adolescent↗

PubMed1979