Sensitive titration of antibodies and antigens using erythro-immunoassay.
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Animals↗
Biomedical subjects
S Avrameas
Publications and source records attributed to S Avrameas.
[Bulimia and autoimmunity].
In the first part of this study, we investigated the rate of natural autoantibodies, in a sample of 31 female inpatients with bulimia nervosa according to DSM III-R criteria. The control (age and sex matched) group consisted in high school students including 10 females without eating disorders, depressive disorder or immunological disease. We investigated especially natural autoantibodies reacting with compounds of the central nervous system (Dopamine, Dopamine beta Hydroxylase, Serotonin). Our first conclusion is that there is a lower level of these natural auto-antibodies among female patients with bulimia nervosa. In the second part of the study, we have especially investigated the correlation between impulsivity in bulimia nervosa and the rate of natural autoantibodies against serotonin.
Adolescent↗
[Properties of immunoadsorbents prepared by antigen coupling to glutaraldehydeactivated polyacrylamide gel, BrCN-activated Sepharose and by copolymerization of antigens by glutaraldehyde].
Three types of immunoadsorbents were synthetized by antigen coupling to glutaraldehyde-activated polyacrylamide gel, BrCN-activated sepharose 4B and protein insolubilization using glutaraldehyde as a cross-linking agent. Different concentrations of rabbit gamma globulin, bovine serum albumin, soybean trypsin inhibitor and bovine ribonuclease were used as antigens and some properties of such immunoadsorbents were studied. Using 125J labelled antigens it was shown that glutaraldehyde-activated polyacrylamide gel (Bio-Gel P-300) couples 81-94% of the antigen added in a concentration of 0.5-4.0 mg.ml-1 gel and BrCN-activated sepharose 4B bounds 62-88% of the labelled antigen in a concentration of 5.0-20.0 mg.ml-1 gel. These antigen derivatives as well as those obtained by glutaraldehyde protein insolubilization permitted 54-88% of antibodies added with the immune sera to be isolated. There was a significant antigen leakage from sepharose immunoadsorbents after several antisera treatments or after half a year storage but despite of this antigen desorption all types of the immunoadsorbents studied preserved their antibody isolation capacity. Immune sera immunoglobulins nonspecific binding by immunoadsorbents was less on sepharose than Bio-Gel antigen derivatives.
Antigen-Antibody Complex↗