Immunoenzyme techniques: enzymes as markers for the localization of antigens and antibodies.
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Adenoviridae↗
Biomedical subjects
S Avrameas
Publications and source records attributed to S Avrameas.
Specificity of antibodies in single cells after immunization with antigens bearing several antigenic determinants. Study with a new paired staining technique.
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Animals↗
Cellular origins of humoral antibodies.
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Animals↗
Enzymatically actice membranes: some properties of cellophane membranes supporting cross-linked enzymes.
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Aldehydes↗
[Preparation of insoluble protein derivatives and their use for isolating antibodies, antigens, enzymes and enzyme inhibitors].
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Aldehydes↗
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
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Aldehydes↗
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.
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Aldehydes↗
Glutaraldehyde, cyanuric chloride and tetrazotized O-dianisidine as coupling reagents in the passive hemagglutination test.
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Aldehydes↗
Indirect immunoenzyme techniques for the intracellular detection of antigens.
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Alkaline Phosphatase↗
Isolation of viruses using specific immunoadsorbents.
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Adsorption↗
Localization of virus antigens by enzyme-labelled antibodies.
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Adenoviridae↗
Ultrastructural localization of SV40T antigen with enzyme-labelled antibody.
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Animals↗
Ultrastructural localization of intracellular immune globulins in plasma cells and lymphoblasts by enzyme-labeled antibodies.
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Alkaline Phosphatase↗
[Association of immunofluorescence and immunoenzymology for the detection of intracellular antibodies].
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Animals↗
[Detection of antibodies and antigens by means of enzymes].
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Animals↗
[Study with the electron microscope of antibody formation with the aid of alkaline phosphatase used as an antigen].
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Alkaline Phosphatase↗
Ultrastructural localization of antibody in differentiating plasma cells.
Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.
Animals↗
Ultrastructural localization of antibody by antigen label with peroxidase.
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Animals↗