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Biomedical subjects

S Avrameas

Publications and source records attributed to S Avrameas.

At least 199 records · Page 11Linked to original sources
PubMed1973

Detection of plasma membrane carbohydrates with lectin peroxidase conjugates.

With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem.14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties on the surface of unfixed, or paraformaldehyde-fixed rat lymphoid cells. D-Galactose, or d-galactose containing disaccharides inhibited the interaction between ricin peroxidase and lymphoid cell surface; also, N-acetylglucosamine inhibited the wheat germ peroxidase-lymphoid cell interaction, but N-acetylgalactosamine failed to inhibit the reaction between phytohemagglutinin peroxidase and the surface of lymphoid cells.

Animals↗
PubMed1973

Intracellular distribution of antibody in immunocytes responding to primary challenge with horseradish peroxidase.

A new improved technic was used to follow the development of, and the intracellular antibody distribution in antiperoxidase antibody-forming cells (AFC) of the mouse popliteal lymph nodes responding to primary stimulation with horse-radish peroxidase (HRP). The first AFC were found 6 to 8 days after immunization and were all plasma cells. Antibody was concentrated in the Golgi complexes and in a few cisternae of rough endoplasmic reticulum. Subsequently, an increasing proportion of the AFC were filled with antibody, and with time the numbers of full cells and the intensity with which they stained increased. Kinetic studies of the cell changes in the lymph node medulla suggest that lymphoid cells proliferate, differentiate into plasma cells, and are then recruited as AFC. Furthermore, it was concluded that the changing intracellular distribution of antibody represents the gradual filling of the AFC with specific antibody.

Animals↗
PubMed1973

Intracellular distribution of antibody in immunocytes responding to secondary challenge with horseradish peroxidase.

A new improved immunoperoxidase method was used to study the antiperoxidase antibody-forming cells (AFC) in the medullae of mouse popliteal nodes after a second challenge with horseradish peroxidase (HRP). Two populations of AFC were found: a) A stable nondividing background population of mature plasma cells whose content of antibody increased 3 days after challenge and b) A new population of cells which increased in number exponentially between 18 hours and 5 days after challenge. In contrast with the first response, the new AFC included many stimulated lymphoid cells, significant numbers of small lymphocytes, and plasma cells. The results suggest that the morphology of the AFC found in the first and second responses reflects the stage in their developmental cycle at which the precursor cells are recruited into the AFC population. More than 90% of the AFC arising between 18 hours and 3 days after challenge had antibody in their perinuclear cisternae, and their cisternae of rough endoplasmic reticulum were rapidly filled with antibody. The striking differences between the intracellular localisation of antibody during the first and second responses are discussed.

Animals↗
PubMed1972

Modulation of cell behavior in vitro by the substratum in fibroblastic and leukemic mouse cell lines.

Fibroblastic and leukemic mouse cell lines were cultivated on a bovine serum albumin polymer covered with basic or acidic substances. Contact inhibition of fibroblast movement (cell overlapping) and division varied depending on prior treatment of the albumin polymer. The leukemic cells, which normally grow in suspension, attached and spread out on the polymers and could be carried as a monolayer. Preparation of surfaces capable of modulating different parameters of cell behavior in vitro is thus possible.

Aldehydes↗