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Biomedical subjects

S Asari

Publications and source records attributed to S Asari.

At least 145 records · Page 8Linked to original sources
PubMed1983

Disappearance and development of cerebral aneurysms in moyamoya disease. Case report.

The authors report a case of multiple cerebral aneurysms associated with moyamoya disease, in which the reduction and disappearance of bilateral internal carotid artery aneurysms and the new development and enlargement of bisilar artery aneurysms were demonstrated on sequential cerebral angiograms. Possible mechanisms of the spontaneous disappearance and development of cerebral aneurysms in association with moyamoya disease are discussed.

Adult↗
PubMed1983

[Susceptibility of clinical isolates to cefotaxime].

Susceptibilities of 737 strains of 19 species of bacteria to cefotaxime (CTX) were determined based on the inhibition zone diameter obtained by the single-disc method. Four categories were assessed. 1. Susceptibility of clinical isolates to CTX and 6 other antibiotics Against most strains, CTX showed higher antibacterial activity than other drugs (CET, ABPC, SBPC, CMZ, GM, AMK), especially for S. pneumoniae, S. pyogenes and S. agalactiae. Furthermore, CTX was more active than the other antibiotics against E. coli, Indole (+) Proteus, P. mirabilis, Klebsiella sp., S. marcescens, H. influenzae and E. cloacae. 2. Susceptibility of strains isolated from different clinical materials CTX showed the highest antibacterial activity against most strains isolated from sputum, urine, pus, blood and cerebrospinal fluid. However, CTX was occasionally less than potent AMK and GM against strains isolated from bile. Against P. aeruginosa strains derived from clinical materials, the following results were obtained: AMK greater than CFS, FOM greater than CTX greater than GM greater than SBPC 3. Susceptibility of clinical isolates in 7 different fields CTX was the most active antibiotic tested in the fields of internal medicine, pediatrics, urology, obstetrics & gynecology, dermatology and otorhinolaryngology. But in surgery, CTX was less potent than GM and AMK. 4. Susceptibility of clinical isolates of inpatients and outpatients CTX showed excellent activity against many beta-lactamase resistant strains isolated from patients.

Bacteria↗
PubMed1983

Correlation of peripheral T gamma and K lymphocytes in normal subjects and patients with autoimmune thyroid diseases.

K lymphocytes are thought to be included in the T gamma lymphocyte subpopulation, but the reports on the relation between T gamma and K lymphocyte counts in human peripheral blood are not available. Peripheral T gamma and K lymphocytes were enumerated simultaneously by EA rosette formation and ADCC plaque formation, respectively, in normal subjects and in patients with autoimmune thyroid disease. In thyrotoxic Graves' disease, the percentages of both T gamma and K lymphocytes were lower than in normal subjects. No abnormalities in T gamma and K lymphocytes were observed in euthyroid patients with Graves' disease and with Hashimoto's disease. The percentages of T gamma lymphocytes were higher than those of K lymphocytes and these 2 lymphocyte subpopulations showed significant correlations within groups of patients with Graves' disease and Hashimoto's disease and in normal subjects. The slope of the regression line for the group with Hashimoto's disease was normal but that for the group with Graves' disease was steeper than that for normal subjects. These data indicate that T gamma lymphocytes and K lymphocytes are in overlapping subpopulations of Fc gamma receptor-bearing lymphocytes in human peripheral blood and suggest that there is an abnormal distribution of K lymphocytes in the T gamma lymphocyte subpopulation in Graves' disease.

Autoimmune Diseases↗
PubMed1983

[Successful radiotherapy in spontaneous carotid-cavernous sinus fistula--a case report].

The authors reported a case of spontaneous carotid-cavernous sinus fistula fed by meningohypophyseal trunk of the internal carotid artery, that completely disappeared following cobalt 60 irradiation to the region of fistula (27 days; total dose, 4,100 rad). A 65-year-old man was admitted to our hospital on May 25, 1982, with a two-week history of progressive prominence of the left eye, diplopia on left lateral gaze, and continuous intracranial bruit. He first noted redness of the left eye without apparent cause such as head trauma. On admission, slight nonpulsative exophthalmos, periorbital edema, chemosis, and venous congestion of the conjunctiva of the left eye were noted. The pupils were reactive and equal in size, and the left abducent nerve palsy was observed. Fundoscopic examination disclosed to be normal. No bruit was heard over the left orbital region. Left carotid angiography on May 28 demonstrated dural internal carotid-cavernous sinus fistula fed by meningohypophyseal trunk, and drained into superior and inferior ophthalmic veins and basilar venous plexus. Though the patient was treated only conservatively, symptoms became worse to decrease visual acuity. On July 14, we started cobalt 60 irradiation to the region of fistula (27 days; total dose 4,100 rad). Symptoms gradually improved about halfway through irradiation. After completion of irradiation, almost complete improvement of visual and ocular symptoms was observed except the left abducent nerve palsy. Repeated angiography on Aug. 20 revealed complete disappearance of fistula. Three months after treatment, no recurrence of symptoms was observed and the abducent nerve palsy persisted without improvement.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1982

Effects of various isolation methods for human peripheral lymphocytes on T cell subsets determined in a fluorescence activated cell sorter (FACS), and demonstration of a sex difference of suppressor/cytotoxic T cells.

The effects of various methods for removal of monocytes and residual erythrocytes from human peripheral blood mononuclear cells (PBMC) on T cell subsets were examined. T cell subsets were determined in a fluorescence activated cell sorter (FACS) using fluorescein-conjugated monoclonal antibodies (anti-Leu-1, anti-Leu-2a and anti-Leu-3a antibodies). Removal of monocytes by methods based on adherence or phagocytosis decreased yields of lymphocytes and caused changes in the percentage and/or the fluorescence intensity of T cell subsets. Exclusion of monocytes from the FACS analysis by setting of the scatter gates was incomplete (about 80%). Removal of residual erythrocytes after Ficoll separation by ammonium chloride treatment also changed the percentage of T cell subsets. A method using PBMC without removal of monocytes and erythrocytes was chosen as best and simplest for routine use in FACS analysis of lymphocytes. Erythrocytes could be excluded from the FACS analysis by setting the scatter gates and the percentage of T cell subsets was corrected after measurement of monocytes, identified by peroxidase staining. The reproducibility of measurements of T cell subsets made at different times was examined using PBMC obtained from the same healthy man during 12 weeks. For standardization of the assay, the peak positions of scatter and fluorescence intensity of each PBMC labeled with anti-Leu-3a were adjusted to standard values in each FACS analysis. Under these conditions, variations of other parameters of this standard PBMC were very small in 12 different assays. Using this standard PBMC, satisfactory, reproducible results were also observed on PBMC obtained from another normal subject. Therefore, this standard PBMC labeled with anti-Leu-3a was used as a standard in FACS analysis. Under these accurately standardized conditions, it was demonstrated that the peak position of fluorescence intensity of Leu-2a (suppressor/cytotoxic T) cells was significantly lower (P less than 0.01) in women than in men.

Adult↗
PubMed1982

A microcytotoxicity assay for thyroid-specific cytotoxic antibody, antibody-dependent cell-mediated cytotoxicity and direct lymphocyte cytotoxicity using human thyroid cells.

A microcytotoxicity assay for detection of thyroid-specific complement-dependent cytotoxic antibody (cytotoxic antibody), antibody-dependent cell-mediated cytotoxicity (ADCC) and direct lymphocyte cytotoxicity was developed using human, thyroid epithelial cells as targets. Thyroid tissue was obtained from patient with Graves' disease and was treated with collagenase and then trypsin. The red blood cells, interfollicular fibroblasts and infiltrating lymphoid cells in thyroid tissue from patients with Graves' disease could be removed by this procedure. To obtain entirely single cells as target cells, the suspension of dispersed thyroid epithelial cells was allowed to stand for 1 h at 4 degree C in culture medium to allow cell clumps to settle. For assay of cytotoxic antibody, a mixture of the single target cells, patient's serum and human complement was incubated in microwells for 18 h. After removing the detached cells, remaining target cells in the wells were fixed, stained and counted to assess cytotoxicity. For assay of ADCC and of direct lymphocyte cytotoxicity, target thyroid cells were precultured in the microwells for 18 h. Then effector cells with or without patient's serum were added and cultured further for 24-72 h. Cytotoxicity was assessed as described above. Adherence of effector cells to target thyroid cells sometimes disturbed the enumeration of target cells when the effector/target cell ratio was high. With this microassay system 3 different cytotoxic immune reactions against human thyroid cells could be measured quantitatively at the same time on 2-3 ml blood samples.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1982

Minimum dose contrast bolus in computed angiotomography of the brain.

We have carried out basic investigations of an intravenous minimum dose bolus (MinDB) injection method for cerebral computed angiotomography by comparing blood iodine concentrations with high resolution computed tomography (CT) images of cerebral blood vessels. With the GECT/T 8800 scanner, a MinDB injection of 1 ml/kg of 60% Conray at a rate of 2 ml/s made it possible to increase the blood iodine concentration in the carotid artery to more than 15 mg/ml for about 20 s. Computed tomography performed during this period enabled us to obtain clear images of the circle of Willis, including other main cerebral arteries, deep veins, and cortical and lenticulostriate arteries. Cerebral computed angiotomography using this method should be useful for noninvasive screening or detection of cerebrovascular lesions themselves, such as cerebral aneurysms, cerebrovascular obstructions, arteriovenous malformations, and moyamoya disease, and should also be useful for determining the anatomical relationship between the cerebral blood vessels and other parenchymal or space occupying lesions.

Carotid Arteries↗
PubMed1982

Normal anatomy of cerebral vessels by computed angiotomography in the coronal, Towne, and semisagittal planes.

This report presents a detailed analysis of the normal cerebrovascular anatomy on the modified coronal, Towne (half-axial), and semisagittal computed tomographic (CT) images. Direct multiplane CT scans were obtained from fresh cadavers injected with gelatinous iodine solution. The modified coronal plane is optimal for delineation of the internal carotid bifurcations and the anterior and the middle cerebral arteries, and for identification of the lenticulostriate arteries and the angiographic sylvian point. The Towne plane is optimal for delineation of the vertebrobasilar arterial system. Thorough knowledge of the normal appearance of cerebral blood vessels in biplane or multiplane computed angiotomography is a prerequisite for understanding the spatial relationships of the abnormal cerebrovascular anatomy.

Basilar Artery↗
PubMed1982

Decrease of immunoglobulin G-Fc receptor-bearing T lymphocytes in Graves' disease.

Peripheral immunoglobulin G (IgG)-Fc receptor-bearing T lymphocytes (T gamma cells) in patients with autoimmune thyroid diseases and destruction-induced thyrotoxicosis were assayed by measuring erythrocyte antibody-rosette formation using a microplate technique. In untreated patients with Graves' disease, the percentage of T gamma lymphocytes (mean +/- SD, 8.9 +/- 3.9%; n = 15) was significantly lower (P less than 0.001) than that in normal controls (21.7 +/- 9.1%; n = 35). A similar decrease was found in thyrotoxic patients with Graves' disease under antithyroid drug therapy (12.1 +/- 5.6%; n = 11; P less than 0.01). Normal percentages were observed in euthyroid patients with Graves' disease under antithyroid drug therapy or in remission. In patients with Hashimoto's disease and destruction-induced thyrotoxicosis, the percentage of T gamma lymphocytes was similar to that in normal controls. The percentage of T gamma lymphocytes was significantly correlated inversely with the serum T4 level, the free T4 index, the T3 level, and the free T3 index in patients with autoimmune thyroid diseases, excluding those with Hashimoto's disease with destructive thyrotoxicosis. There were no correlations between T gamma lymphocytes and other variables, such as goiter size, the titer of antithyroid antibodies, or proptosis, in a group of untreated cases of autoimmune thyroid disease. The decreased proportion of T gamma lymphocytes in thyrotoxic patients with Graves' disease may be related to the perpetuation of thyrotoxicosis in patients with Graves' disease.

Adolescent↗
PubMed1982

Delineation of unruptured cerebral aneurysms by computerized angiotomography.

Unruptured aneurysms were diagnosed in 15 of 86 patients with cerebral aneurysms during 2 years beginning in April, 1979. One patient with severe head injury was excluded from the series. Fifteen aneurysms in the other 14 patients were first detected by computerized angiotomography. Six aneurysms were located in the middle cerebral artery, three in the upper half of the basilar artery, two in the anterior communicating artery, two in the posterior cerebral artery, and two at the internal carotid-posterior communicating artery junction (both in the same patient). Three were smaller than 5 mm, eight were between 6 and 10 mm, and four were larger than 10 mm. The noncontrast computerized tomography (CT) findings associated with 15 aneurysms were as follows: five showed defects in the basal cistern of Sylvian fissure, four were calcified or high-density masses, and in the six instances there was no evidence of an aneurysm. Unruptured aneurysms may be suggested by a well demarcated, round, isodense mass which forms a defect in the basal cistern of Sylvian fissure on a plain CT image, and are highly and homogeneously enhanced by computerized angiotomography. A carotid artery blood iodine level of 15 mg/ml is required to obtain clear images. The authors conclude that computerized angiotomography is useful in the delineation of unruptured aneurysms.

Adult↗
PubMed1982

[Multiplane postmortem cerebral computed angiotomography--Part I. Normal anatomy of cerebral vessels on the axial plane (author's transl)].

Cranial computed tomography has been mainly used for detection of the parenchymal space-occupying lesion, and has had the limitation of detection of the cerebrovascular lesion itself because of low CT resolution. However, high resolution CT scanners have recently brought us the possibility of definition of fine cerebral vessels on CT images using an appropriate injection method of contrast agents (cerebral computed angiotomography). This paper concerns the normal anatomy of the cerebral vasculature on CT images using 9 fresh cadavers with normal intracranial structures. They received the postmortem injection of contrast agents through bilateral common carotid and vertebral arteries, and were undertaken the multiplane CT scanning with the axial, modified coronal, Towne (half-axial) and the semisagittal projections using GE-CT/T 8800 (9.6 sec scanning time, 320 x 320 matrices). The normal anatomy of cerebral vessels on the axial plane, obtained at the levels 10 to 90 mm above the canthomeatal line, is presented in this paper. Main visualized vessels in the posterior fossa were the basilar artery, the cranial loop of the PICA, the AICA from the origin to the hemispheric branches including the meatal loop in the cerebellopontine angle cistern, and the SCA from the ambient and quadrigeminal segments to the lateral marginal and superior hemispheric branches. The circle of Willis and other main cerebral arteries were clearly visualized with their small branches, for example, the posterior communicating, the anterior choroidal and the lenticulostriate arteries. Deep cerebral veins were also visualized at the levels of the midbrain, the middle and the roof of third ventricle, and the body of lateral ventricle. Postmortem cerebral computed angiotomography provided us not only the precise anatomy of the cerebral vasculatures, but also their anatomic relations with the surrounding structures such as cerebral parenchyma, ventricles, cisterns and other subarachnoid spaces. On the clinical point of view, cerebral computed angiotomography can be applied to the screening system of vascular lesions themselves such as asymptomatic aneurysms, arteriovenous malformations, and Moyamoya disease.

Brain↗
PubMed1982

[A case report of traumatic intracerebral pneumocephalus with interesting CT cisternographic findings (author's transl)].

A 19-year-old boy suffered from headache and intermittent CSF rhinorrhea, was admitted to Matsuyama Shimin Hospital on June 1, 1979. Two months prior to admission he had had a frontal head injury with confusion and the right nasal bleeding. Plain skully x-ray films and biplane (axial & coronal) CT revealed intracerebral pneumocephalus in the right frontal lobe with depressed basal skull fracture into the right ethmoid sinus. Clinical conservative courses of intermittent CSF rhinorrhea, headache and vomiting were related to the changes of the air shadow on plain skull films. Preoperative metrizamide CT Cisternography was done on July 5. Sequential CT cisternograms demonstrated ventricular reflux at 1 hr and partial obstruction of the basal cistern and supratentorial subarachnoid space at 3 hr, which attributed to the mass effect of the air cysts. They also demonstrated an interesting finding, the accumulation of metrizamide into the intracerebral air cyst at 3 hr, suggesting transependymal penetration of contrast medium. At 24 hr, the air cyst decreased in size and the metrizamide disappeared. CT cisternograms 8 months after the surgery showed no evidence of air cyst but remained a low dense porencephalic cyst. Ventricular reflux was seen at 3 and 6 hr but there was no accumulation of metrizamide into the cyst at any hr. Filling patterns of the basal cistern and supratentorial subarachnoid space returned normal except the defect in the anterior interhemispheric cistern. Transependymal penetration of metrizamide in this case can be explained by the mechanism of pressure gradient between the ventricle and the air cyst. Thus the postoperative CT cisternograms showed no penetration because of the absence of pressure gradient, whereas the ventricular reflux and the same ependymal septum still remained. No mention has been made about the CSF flow dynamics in intracerebral pneumocephalus and their relation to the CSF rhinorrhea in previous literature. Intermittent CSF rhinorrhea of this case will attribute to the CSF accumulated in the air cyst as mentioned above.

Adult↗