Gene mapping using 3H-labeled heterologous probes.
Explore the source record for details and available documents.
Animals↗
Biomedical subjects
S A Wilcox
Publications and source records attributed to S A Wilcox.
A eutherian X-linked gene, PDHA1, is autosomal in marsupials: a model for the evolution of a second, testis-specific variant in eutherian mammals.
We report the cloning and mapping of a gene (PDHA) for the pyruvate dehydrogenase E1 alpha subunit in marsupials. In situ hybridization and Southern blot analysis show that PDHA is autosomal in marsupials, mapping to chromosome 3q in Sminthopsis macroura and 5p in Macropus eugenii. Since these locations represent a region that was translocated to the p arm of the human X chromosome following marsupial/eutherian divergence, we suggest that the marsupial PDHA gene is homologous to PDHA1, the somatic eutherian isoform located on human Xp and mouse X. Only one copy of PDHA is found in marsupials, whereas a second, testis-specific, intronless form is observed in eutherian mammals. We also suggest that translocation of PDHA to the eutherian X chromosome, which is inactivated during spermatogenesis, led to the evolution of a second testis-specific locus by retroposition to an autosome.
Amino Acid Sequence↗
Naming as a function of linguistic form-class and object categories.
We examined whether children rely on linguistic information (i.e. mass vs. count nouns) or object category information (i.e. objects vs. substances) when they name things. A grinder test was used, in which substances (e.g. water) maintain identity through transformation but objects (e.g. a cup) do not. Thirty children aged three through six were asked if the same name could be used for the same item after transformation. The items included pairs of amorphous substances and discrete objects (e.g. water--a cup), perceptually similar discrete objects (e.g. chalk--a crayon), and food items (e.g. corn--a bean). Children accepted the same name for food, ignoring linguistic information, and for objects, relying on linguistic information. In Experiment 2, 32 children aged five through eight were asked if the same name could be used for unfamiliar hardware and food items after transformation when they were labelled by nonsense mass and count nouns. Children tended to use the same name for food, relying on perceptual information. These results are discussed in terms of the active conceptualization about names of objects in relation to object characteristics.
Child↗
Marsupial Y chromosome encodes a homologue of the mouse Y-linked candidate spermatogenesis gene Ube1y.
The mammalian subclass Theria consists of infraclasses Metatheria (marsupials) and Eutheria ('placentals') which diverged from each other 120-150 million years before present (Myr BP). Both infraclasses have Y chromosome-dependent testis determination but direct molecular evidence linking the Metatherian and Eutherian Y chromosomes is lacking. Comparative analyses indicate that three mammalian genes have remained Y-linked for at least 80 Myr, since the divergence of the Eutherian orders from a common ancestor. These are Zfy, a gene encoding a transcription factor of the zinc-finger type; Sry, the putative primary testis-determining gene; and Ube1y (formerly Sby or A1s9Y-1), a candidate for the mouse spermatogenesis gene Spy, encoding a ubiquitin-activating enzyme E1 homologue. Although in marspials Zfy homologues are autosomal, a Y homologue of Sry has recently been isolated. We report here the identification of a functional marsupial Y-linked homologue of the murine Ube1y gene establishing that Metatherian and Eutherian Y chromosomes diverged from a common ancestor. This extreme conservation indicates that Ube1y plays a critical role in male development.
Amino Acid Sequence↗
Statistical analysis of in situ hybridization data: derivation and use of the zmax test.
There are many situations in which grain distributions resulting from in situ hybridization of radioactively labeled probes to unique genes should be subjected to a statistical analysis. However, the problems posed by analysis of in situ hybridization data are not straightforward, and no completely satisfying method is currently available. We have developed a procedure in which the major and any number of minor site(s) of hybridization may be specifically located and the significance of each tested. This zmax procedure first tests the overall distribution for departure from randomness and then identifies significantly overlabeled whole chromosomes (or chromosome arms or other large segments), a process that may be repeated to pinpoint significantly overlabeled regions within these chromosomes. We describe in detail the derivation of the zmax statistic, present tables of significant zmax levels, and show with examples how zmax is used in tests of significance of in situ hybridization data.
Animals↗
The prevalence of mandibular dysfunction. Part I: Sex and age distribution of related signs and symptoms.
This study concludes the following: 1. Mandibular dysfunction is extensive. 2. Age and sex differences are apparent with various signs and symptoms. 3. Women and younger patients tend to respond positively more often than men and older patients to questions relating to subjective TMJ and muscle symptoms. 4. Objective TMJ and occlusal signs show little age or sex differentiation. 5. Positive responses to questions regarding bruxism were more common in men. 6. Age and sex differences in the prevalence of mandibular dysfunction may be influenced by the method of investigation (anamnestic versus clinical examination) and by the signs and symptoms selected to be representative of this disorder. When compared with the findings of earlier investigations, the conclusions suggest that social, cultural, psychologic, sex, and age differences contribute to the responses to questions pertaining to mandibular dysfunction. However, this may not be an accurate reflection of the prevalence of mandibular dysfunction since the objective signs do not show the age and sex differences that the subjective symptoms indicate. Therefore, additional studies are needed to correlate all data to establish a more reliable profile of both signs and symptoms of mandibular dysfunction.
Adolescent↗
Helping minority and low-income psychotherapy patients "Tell It Like It Is".
The development and field testing of an instructional slide/cassette program entitled "Tell It Like It Is" are described. The program is a major component of a three-year study to assess the effects of patient orientation, therapist orientation, and the interaction of the two upon the psychotherapy process and outcome for working class and low income Mexican American, Black American, and Anglo American patients.
Black or African American↗
Carbon rod atomization analysis: application to in vivo lymphocyte gold quantitation.
Explore the source record for details and available documents.
Binding Sites↗
Effect of chrysotherapy on parameters of immune response.
Thirty-nine subjects with classical or definite rheumatoid arthritis received weekly intramuscular gold sodium thiomalate (GST) to sustain gold blood levels of more than 320 microgram/dl. Statistical analysis revealed significant declines from pre-treatment values for IgM, IgG, and IaA. Rheumatoid factor titer decreased in 29 of 39 subjects, 15 becoming seronegative. Circulating lymphocytes decreased by 27%. The maximal suppressive effect on IgM was not achieved until the 3rd and 4th years of GST administration. Auranofin (AF) 6 mg/day was administered to 15 patients for an average interval of 45 weeks. In vitro and in vivo suppression of lymphocyte mitogen response with AF was more rapid in onset and significantly greater than with GST. Suppression of dinitrochlorobenzene skin sensitization was observed in AF patients. The clinical response in GST treated subjects correlated with suppression of immunoglobulins, rheumatoid factor titer, and circulating lymphocytes. A significant decline in these variables was not achieved for a corresponding interval with AF treatment. It is suggested that chrysotherapy may be applied more widely to immunologically-mediated disorders and perhaps be used to affect selectively B versus T mediated dysfunction.
Antibody Formation↗
A tale of two representations: the misinformation effect and children's developing theory of mind.
The present research investigates representational ability as a cognitive factor underlying the suggestibility of children's eyewitness memory. The misinformation effect is used as an index of children's suggestibility, and performance on the false belief task is used as an assessment of children's representational abilities (N = 117). Analyses that considered the effect of representational ability and general memory ability on children's susceptibility to misleading information showed that differences in representational ability and general memory ability predicted participants' susceptibility to misleading information. These results demonstrate that the eyewitness memory of children who lack either multirepresentational abilities, sufficient general memory abilities, or both (i.e., most 3- and 4-year-olds) is less accurate than the eyewitness memory of children with both multirepresentational abilities and sufficient memory abilities (i.e., most 6-year-olds and adults). Thus, it appears that the earliest age at which children's eyewitness memory can be considered to be similar to that of adults is 6 years of age, when children's mental representational abilities are similar to those of adults. These results suggest that one factor underlying children's vulnerability to misleading information is the number of representations of an event that they can simultaneously hold and compare.
Child↗