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Biomedical subjects

S A Reed

Publications and source records attributed to S A Reed.

8 recordsLinked to original sources
PubMed2002

Time-resolved fluorescence immunoassay (TRFIA) for the detection of Escherichia coli O157:H7 in apple cider.

An immunoassay based on immunomagnetic separation and time-resolved fluorometry was developed for the detection of E. coli O157:H7 in apple cider. The time-resolved fluorescent immunoassay (TRFIA) uses a polyclonal antibody bound to immunomagnetic beads as the capture antibody and the same antibody labeled with europium as the detection antibody. Cell suspensions of 10(1) to 10(8) E. coli O157:H7 and K-12 organisms per ml were used to test the sensitivity and specificity of the assay. The sensitivity of the assay was 10(3) E. coli O157:H7 cells with no cross-reaction with K-12. Pure cultures of E. coli O157:H7 (10(1) to 10(5) CFU/ml) in apple cider could be detected within 6 h, including 4 h for incubation in modified EC broth with novobiocin and 2 h for the immunoassay. When apple cider was spiked with 1 to 10(3) CFU/ml of E. coli O157:H7 and 10(6) CFU/ml of K-12, our data show that the high level of K-12 in apple cider did not impede the detection of low levels of O157:H7. The minimum detectable numbers of cells present in the initial inoculum were 10(2) and 10(1) CFU/ml after 4- and 6-h enrichment. The TRFIA provides a rapid and sensitive means of detecting E. coli O157:H7 in apple cider.

Antibodies, Bacterial↗
PubMed2001

Medical and psychological aspects of infertility and assisted reproductive technology for the primary care provider.

Couples attempting to conceive are requiring more assisted reproductive technology. Infertility may be associated with delayed onset of marriage and childbearing, smoking and alcohol excess, physiological factors such as endometriosis and varicocele, or a cause that is not identified. The psychological needs of couples, however, are often overlooked. Primary care providers can serve as the initial information source and guide for the couple struggling with infertility. In a managed care environment, a primary care provider can provide a considerable amount of education, referral for stress management and counseling, and a small portion of the medical evaluation before referring to a reproductive specialist. This overview is intended to help primary care providers and couples achieve an educated and less stressful assisted reproductive technology experience. It is not meant to circumvent the need for immediate referral to a reproductive specialist for evaluation and treatment of this very complex intervention.

Female↗
PubMed1990

General strategies to modify/refocus unproductive behaviors.

The management of problem employee behaviors is often the most difficult component of the nurse manager's role. Discipline is viewed as a negative task rather than as an opportunity to refocus employee energies or modify unworkable behaviors. The nurse manager must deal with his or her own reactivity toward the employee who exhibits problem behaviors. The nurse manager's approach will have a major impact on determining the outcome of the interventions. The use of confrontation, refocusing, or modifying problem employee behavior are general strategies to assist in optimizing employee resources to meet the mission of the institution and the functioning of the unit.

Behavior Therapy↗
PubMed1990

Specific strategies: interventions for identified problem behaviors.

Negativism, complaining, underachievement, game playing, passive-aggressive behavior, and workaholism constitute a repertoire of problem employee behaviors that impact on the productivity and morale of the work environment. Responding appropriately to the employee who presents with any of these behaviors is a formidable challenge to the nurse manager. Understanding the etiology of unmet needs, psychosocial dynamics (as discussed in Chapter 1) and variety of interventions can empower the nurse manager to achieve success in these difficult interactions.

Attitude of Health Personnel↗
PubMed1979

Public relations audit.

Explore the source record for details and available documents.

Community-Institutional Relations↗