Biomedical subjects

S A Neifakh

Publications and source records attributed to S A Neifakh.

33 records · Page 2Linked to original sources

PubMed1976

[Identification of hexokinase synthesizing polyribosomes in human normal and tumor tissues].

Comparative study of concentrations of growing hexokinase subunits polypeptide chains in the polyribosomes from normal and tumour human stomach tissue is studied using specific antiserum to hexokinase isoenzyme from human stomach tumour tissue. It is shown by two different methods (chromatography on Sepharose 46 and sucrose concentration gradient analysis) that the content of hexokinase polypeptides in stomach tumour is several times higher than in homologous normal tissue. The acta obtained show that the high activity of hexokinase in tumour tissues is due to the increased synthesis rate of the enzyme.

Hexokinase↗

PubMed1975

Letters to the editor: On the transfer of nuclear RNA into isolated mitochondria. Further evidence for template properties of nuclear RNA taken up by isolated mitochondria.

Some properties of nuclear RNA taken up by isolated mitochondria during aerobic incubation were studied. Nuclear RNA fraction re-isolated from incubated mitochondria was characterized by homogeneous sedimentation distribution in sucrose gradient (7-9S) and by absence of extended secondary structure. In this respect the RNA fraction differed from the nuclear RNA added to mitochondria and was similar to in vivo rapidly labelled mitochondrial RNA. The hybridization pattern of re-isolated RNA with nuclear DNA is consistent with the presence of transcripts from unique DNA sequences in this RNA fraction.

Aerobiosis↗

PubMed1975

On the defect of synthesis ceruloplasmin in the liver polyribosomes in Wilson's disease.

Comparative immunochemical analysis of ceruloplasmin-synthesizing polyribosomes in liver biopsies from control subjects and homozygous carriers of the Wilson's mutation was performed. According to I125-antibody binding data, the amount of ceruloplasmin-forming liver polysomes in patients with Wilson's disease was 10--20 times lower than that in non-Wilson patients. Correspondingly, the pulse labeling of ceruloplasmin polypeptides was decreased several-fold in the cell-free liver preparations from patients with Wilson's disease.

Amino Acids↗

PubMed1974