Biomedical subjects

R Zhang

Publications and source records attributed to R Zhang.

At least 433 records · Page 24Linked to original sources

PubMed1995

[The prognostic significance of lymph node metastasis in surgical resection of esophageal cancer].

The metastatic rate and degree of lymph node (LN) were evaluated and the relation between prognostic significance and LN metastasis (M) was analysed through a retrospective study of 474 patients undergoing resections of non-irradiated esophageal carcinoma. In patients with LNM, the LNM rate was 44.5%. A total of 5382 LNs were resected and examined pathologically. 690 of them were metastatic ones with a LNM degree of 12.8%. The 5-year survival rate of this series was 30.6% (145/474), and was 12.8% (27/211) in patients with LNM and 44.9% (118/263) in those without LNM. We concluded that surgery remains the first choice of treatment for carcinoma of esophagus, and that meticulous LN dissection is an important part of surgical oncology. This is especially true when the cancer process is reasonably localized. However, in more advanced cases of this disease, surgery alone is of limited value in eradicating all cancer compromized tissue, and therefore the routine practice of extensive LN dissection in such cases may not be rewarding.

Adult↗

PubMed1995

[Clinical study on the feasibility of preserving vagal trunks in the surgical treatment of carcinoma of the thoracic esophagus].

The results of preserving vagal trunks in the surgical treatment of 47 patients with the carcinoma of the thoracic esophagus were discussed through a controlled study. There were 20 patients in the test group with vagus nerve being preserved (PG) and 27 in the control group (CG) with vagus nerve being routinely severed. Eighteen patients in PG and 26 in CG underwent radical resections, the remaining patients received palliative surgery. Pathological findings showed that 3 cases (15.0%) in PG and 3 cases (11.1%) in CG had cancerous invasion near the vagal trunks and/or their main branchs close to the primary cancer; and 1 case (5.0%) in PG and 3 (11.1%) in CG the vagal nerves were actually invaded by cancer cells. Determinations of basal gastric acid output (BAO) and half gastric emptying time (T 1/2) revealed that the mean value of BAO (1.93mmol/h) and T 1/2(63.51min.) of the PG were statistically different from those of 0.75mmol/h and > 105min. in CG, but similar to those of the healthy group and the preoperative control values. We consider that it is feasible to preserve vagal trunks in the operation and is beneficial in minimizing of postoperative disturbances of gastric acid output and gastric motility; but the indications of this procedure should only be limited to T1-T2 lesions in order not to jeopardize the radicality of tumor resection.

Adult↗

PubMed1995

[Streptococcus pneumoniae-induced pulmonary consolidation prevented with tumor necrosis factor alpha monoclonal antibody in mouse].

Streptococcus pneumoniae stimulate mouse peritoneal macrophages to release tumor necrosis factor alpha (TNF alpha) in vitro. When penicillin was added to lysate bacterium, TNF alpha release was accelerated. 150 Kunming mice were infected with streptococcus pnumoniae through inspiration. Dynamic changes of TNF alpha concentration in serum and bronchoalveolar lavage fluid were detected; pulmonary pathological changes were also observed. It was found that TNF alpha monoclonal antibody significantly attenuates TNF alpha activity in serum and bronchoalveolar lavage fluid, white blood cell chemotaxis, emigation, and infiltration were inhibited. We conclude that streptococcus pneumoniae infection stimulates TNF alpha release, and TNF alpha is probably the major mediator that causes tissue damage during streptococcus pneumoniae infection. As penicillin accelerates TNFa release, single therapy of penicillin may worse tissue damage.

Animals↗

PubMed1995

Eradication of poliomyelitis: progress in the People's Republic of China.

China and the other countries of the Western Pacific Region have a goal of eradication of wild poliovirus by the end of 1995. In this report we examine the progress made toward eradication through the end of 1993. We examined the information about poliomyelitis and wild poliovirus based on the acute flaccid paralysis surveillance system. The number of reported poliomyelitis cases decreased from 4623 cases in 1989 and 5065 cases in 1990, which occurred during a large nationwide poliomyelitis epidemic, to 538 cases in 1993. Mass supplemental immunization sessions were conducted during the 1991 to 1992 and 1992 to 1993 winters. After the two rounds of supplemental immunizations in the 1992 to 1993 winter, wild poliovirus was not detected for the subsequent 21 months in 22 contiguous provinces in central and northern China, in which 980 million persons reside. In 1993 wild poliovirus was detected in only 5 provinces in southern China and in 2 provinces in the remote Western region; these provinces have only 14% of the total population in China. China is close to achieving its 1995 poliomyelitis elimination goal. Mass supplemental immunizations in children 0 to 3 years old can rapidly eliminate wild poliovirus from large, very densely populated areas, low income rural areas and remote mountainous areas. There appears to be no technical obstacle, even in the most difficult areas, to achieving global eradication of wild poliovirus by the year 2000.

Child↗

PubMed1995

[Surgical treatment of esophageal carcinoma complicated with perforation--a report of nine cases].

Malignant perforation in patients with esophageal carcinoma, a catastrophic life-threatening complication, is usually treated conservatively with very poor prognosis. From December 1992 through November 1994, surgical resections was carried out in 9 patients with perforated esophageal carcinoma of these 9 cases, two received esophagogastrectomy alone and 7 received esophagogastrectomy with synchronous pulmonary resections of various types. One patient died of anostomotic leakage on the 33rd postoperative day. Eight patients recovered uneventfully. Postoperative irradiation therapy was given to two patients: one in the third month due to mediastinal lymph node metastasis and the other is still being irradiated in the first month after operation. On follow-up, six patients had been surviving for 20, 18, 11, 9.5, 8.5 and 8 months after operation, respectively. In this paper options of treatment, pre-operative diagnostic procedures, intra-operative judgement and post-operative management for the perforated esophageal carcinoma are emphasized. The authors stress that the indication of extended operation for the esophageal carcinoma complicated with perforation should be further explored.

Adult↗

PubMed1995

[The relationship between preoperative small airway function tests and postoperative airway infection in patients with esophageal carcinoma].

The article reports the results of preoperative small airway function tests among 116 cases of esophageal carcinoma and its relationship with the postoperative airway infection. Among three main parameters reflecting narrowing of small airway, %V25/HT is the most sensitive one, %MMEF comes next, and %FEV1 the least, Eight-eight of 116 cases without any other postoperative complications were enrolled. The morbidity of postoperative airway infection in this group was 62.5%. They were divided into three groups according to %V25/HT: group I with normal small airway function (%V25/HT > 70%, 26 cases), group II with moderately deviated function (%V25/HT 46-70%, 31 cases), group III with severely deviated function (%V25/HT < 45%, 31 cases). The morbidity rate of postoperative airway infection and the average fever period in the three groups were 50%, 51.6%, 83.3% and 60, 74, 108 hours, respectively. The difference between group I and group III, between group II and group III are significant statistically both in the morbidity rate and the fever period of postoperative airway infection. If divided by different modes of operation, the average postoperative fever perioed in intrathoracic and neck anastomosis group was 79 and 105 hours. The difference between the above two groups is remarkable but not significant statistically. The results come to a conclusion that the patients undergoing larger operation and with more severe abnormal small airway function usually have longer and more severe postoperative airway infection.

Adult↗

PubMed1995

[The protective effects of drugs H and X on liver cold ischemic injury].

Using isolated perfusion rat liver model (IPRL), we studied the protective effects of drugs H and X on rat's liver cold ischemic injury. After the rat's liver was preserved for 12 hours in Collins II solution with the addition of drugs H and X, the necrotic rate of sinusoidal lining cells was significantly lower than that of control group. Within certain range of the dosage of drugs H and X, the necrotic rate of sinusoidal lining cells was in the inverse ratio of the dosage in the Collins II solution. We also found that drugs H and X had obvious protective effect on liver cell redox function. But these drugs could not inhibit tissue from swelling after Collins II solution preservation. The protective mechanisms of the drugs maybe relate to the remoral of free radicals and suppression of Kupffer's cells activity.

Animals↗

PubMed1995

The population association of glucokinase gene with type 2 (noninsulin-dependent) diabetes mellitus in Chinese.

The association of gluckinase (GCK) gene with type 2 (non-insulin-dependent) diabetes mellitus was investigated in 168 Chinese subjects (85 unrelated type 2 diabetics and 83 non-diabetic controls). The microsatellite polymorphism marker, GCK-5', was amplified with polymerase chain reaction. Four alleles were observed in Chinese population with length varying from 137bp to 143bp and the most common one being the 139bp allele 3. In comparison with non-diabetics, allele 4 was significantly increased in type 2 diabetes (10% versus 38, respectively; X2 = 6.773, P = 0.009); genotype 44 and 4X (X denotes any allele other than allele 4) were significantly increased in type 2 diabetes (16% versus 6% respectively; X2 = 6.439, P = 0.011). The frequency difference was also shown in overweight/obese subgroup comparison (X2 = 7.718, P = 0.021), but not in lean/normal-weight subgroup comparison. No differences of age of onset and frequency of positive family history were observed between type 2 diabetic patients with genotype 44 or 4X and those with XX. The risk for type 2 diabetes in Chinese with genotype 44 or 4X was about 3.5 times higher than in Chinese with genotype XX. Therefore, GCK gene was associated with Chinese type 2 diabetes.

Adult↗

PubMed1995

In vivo stability and disposition of a self-stabilized oligodeoxynucleotide phosphorothioate in rats.

The use of antisense oligonucleotides represents a novel, genetically based therapy. The biostability and pharmacokinetics of a 33-mer self-stabilized oligodeoxynucleotide with significant anti-HIV activity was determined in rats after intravenous administration of [35S]oligodeoxynucleotide. Plasma disappearance of the labeled oligodeoxynucleotide could be described by a two-compartment model, with half-lives of 0.54 and 41.44 h. The oligodeoxynucleotide in plasma remained mainly intact. Urinary excretion represented the major elimination pathway, with approximately 27% of the administered dose excreted within 24 h and 57% over 240 h. The majority of radioactivity in urine was attached to degradative products. Fecal excretion was a minor elimination pathway. A wide tissue distribution of the oligonucleotide was observed, with the majority of radioactivity in most tissues being intact. Compared with other linear oligonucleotide phosphorothioates, the self-stabilized oligonucleotide was more stable in vivo, which may be important in development of antisense oligonucleotides as therapeutic agents.

Animals↗

PubMed1995

[Dysgerminoma of the ovary: a clinical retrospective analysis of 60 cases].

OBJECTIVE: To study the treatment methods and the prognostic factors of pure dysgerminoma of the ovary. METHODS: Sixty cases histologically confirmed pure ovarian dysgerminoma treated in our hospital, from May 1959 to Oct. 1992 were retrospectively analysed. Factors mainly studied were: age, symptoms, clinical staging and treatment methods. RESULTS: The median age of this series was 21. The main symptoms were abdominal mass, pain and distension. Thirty two out of 60 cases were stage I, 2 were stage II, 5 were stage III and 21 with recurrences or persistence of the disease. All cases excepting one case received surgery alone were treated by surgery combined with external radiotherapy or chemotherapy. Nine cases died of the disease. The actual 5-year survival rate was 85.17%. The survival rate for patients with postoperative adjuvant chemotherapy is 95% and that for patients with radiotherapy is 76.1%; no statistic significance was shown. CONCLUSIONS: In pure ovarian dysgerminoma surgery combined with radiotherapy or chemotherapy showed high complete remission rates and excellent survival rates. For younger patients and those with gestation desires as well as patients with advanced diseases, adjuvant chemotherapy following surgery might be a better choice.

Adolescent↗

PubMed1995

In situ effects of interferon on human glioma protein kinase C-alpha and -beta ultrastructural localization.

Transmission electron microscopy was used to determine how immunogold labeling of PKC-alpha or -beta is modulated by the antitumor drug IFN (HuIFN alpha-2b) in the cytoplasm, membrane structures, and nucleus of rapidly dividing and confluent human glioma U-373 cells. Results showed that except for nuclear localization, there were no specific cytoplasmic organelles that PKC-alpha or -beta translocated to following HuIFN alpha-2b treatment. Electron micrographs of PKC-beta in proliferating cells depicted 1.34-fold more PKC-beta in the nucleus than in the cytoplasm and a 1-min HuIFN alpha-2b (500 units/ml) treatment transiently increased PKC-beta immunoreactivity in the cytoplasm (1.95-fold) and nucleus (1.97-fold). In confluent cells, incubation with HuIFN alpha-2b for 2 min significantly decreased cytoplasmic PKC-beta immunoreactivity by 37%, and no change was observed in nuclear PKC-beta labeling. PKC-alpha labeling in proliferating cells showed similar immunoreactivity in both control cytoplasm and nucleus. Treatment of proliferating cells with HuIFN alpha-2b for 2 min decreased PKC-alpha in the cytoplasm (59%) and nucleus (44%). In confluent cells, cytoplasmic PKC-alpha labeling decreased 59% at 1 min, 61% at 2 min, and 76% at 10 min of HuIFN alpha-2b treatment. Nuclear PKC-alpha decreased by 65% at 1 min, 80% at 2 min, and 62% at 10 min after HuIFN alpha-2b treatment. Western blots of total PKC-alpha in proliferating and confluent cells and PKC-beta in confluent cells showed similar results. However, Western blots of total PKC-alpha and -beta in proliferating cells did not demonstrate any significant changes in either PKC-alpha or -beta immunoreactivity following 1-min HuIFN alpha-2b treatment. These results suggest that treatment of proliferating U-373 cells with HuIFN alpha-2b for 1 min unfolds and exposes PKC-beta antigenic sites (hinge region) and increases in situ PKC-beta immunogold labeling.

Amino Acid Sequence↗

PubMed1995

[Clinical observation and therapeutic mechanism of blocking agent of substance P nerves in the treatment of perennial allergic rhinitis].

This paper reported the clinical effects and therapeutic mechanism of blocking agent of substance P(SP) nerves on prerennial allergic rhinitis. We applied capsaicin (CAP) in the treatment of 50 cases of perennial allergic rhinitis once a week and 4 times as a therapeutic course. By using highly specific and highly sensitive SP radioimmunoassay, the SP contents in nasal secretions were determined before and after CAP therapy. The results showed that clinical symptoms of allergic rhinitis were obviously relieved and SP content in the nasal secretions was remarkably reduced from 29.444 +/- 14.280pmol/L before CAP therapy to 16.848 +/- 10.622 pmol/L after therapy (P < 0.001). Of the 50 cases, 35 cases (70.0%) showed the best effects, 12 cases (24.0%) better effects and 3 cases (6.0%) no effects. The total effective rate was 94.0%. The study indicated that therapeutic mechanism of CAP on perennial allergic rhinitis is related to the reducing of SP and the blocking of axonal reflex of the SP nerves.

Administration, Intranasal↗

PubMed1995

Pentoxifylline ameliorates pulmonary damage caused by Streptococcus pneumoniae infection in mouse.

Streptococcus pneumoniae stimulated mouse peritoneal macrophage to release tumor necrosis factor-alpha (TNF alpha) in vitro. When penicillin was added into the medium with bacteria, TNF alpha release was accelerated. Pentoxifylline (PTX), a phosphodiesterase inhibitor, significantly attenuated TNF alpha release caused either by Streptococcus pneumoniae or by its lysates. In this experiment, 150 Kunming mice were infected with Streptococcus pneumoniae through inspiration. Dynamic changes of TNF alpha concentration in serum and bronchoalveolar lavage fluid were determined, and pulmonary pathological changes were also observed. It was found that PTX significantly attenuated TNF alpha activity in serum and bronchoalveolar lavage fluid, and inhibited white blood cell chemotaxis, emigration and infiltration. In conclusion, Streptococcus pneumoniae infection stimulates the release of TNF alpha which is probably the major mediater that causes tissue damage during Streptococcus pneumoniae infection. The mechanism is probably that Streptococcus pneumoniae and its lysates activate TNF alpha gene transcription. As penicillin accelerates TNF alpha release, treatment with penicillin alone may aggravate the tissue damage. Combined treatment with PTX may be more reasonable.

Animals↗

PubMed1995

[Comparative study of HCG receptors in rat ovary and testis].

It has been documented that there are specific human chorionic gonadotropin (HCG) receptors in cell membrane of rat ovary and testicle. In order to make a further study on the characteristics of the HCG receptors on rat ovary and testicle, we focused on comparing those two kinds of receptors. Furthermore, we contrasted the testicular cell membrane with its homogenates. Rat ovarian and testicles were homogenized and soniced under ice cold conditions. The homogenates were centrifuged at 12,100 x g for 20 min following filtration. After sucrose density gradient ultracentrifugation (78,000 x g, 2.5h) for the pellets, the interface layers between 37% and 41% were collected. The pellets were subsequently suspended in cold 1mmol/L NaHCO3 solution and recentrifuged at 27,000 x g for 25 min. The results show that the cell membrane of both the rat ovary and testicle can bind to HCG specifically. A small amount of HCG has more evident inhibitory effect on the binding in testis than ovary. Scatchard plot analysis for the binding assay yielded a bound capacity of testicle cell membrane of 4.5 x 10(-5) mol/micrograms protein, Kd of 1.6 x 10(-7) mol/L, whereas it was 0.51 x 10(-9) mol/micrograms protein, a Kd of 1.69 x 10(-6) mol/L in ovarian cell membrane. In comparison with homogenates and cell membrane, the HCG binding activity of later is more sensitive.

Animals↗

PubMed1995

[Anti-promoting and anti-mutagenic actions of G9315].

G9315, a complex extracted from Glycyrrhizae inflata Bat (III), consists of 6 flavonoids with significant antioxidant effects. At 1 mg dose it inhibited the mouse ear edema induced by croton oil, and showed strong anti-promoting effects on two-stage carcinogenesis in mouse skin induced by DMBA plus croton oil. The TPA enhanced 32Pi-incorporation into phospholipid fraction in HeLa cells was inhibited, and the micronuclei in mouse bone marrow cells induced by cytoxan was also depressed.

Animals↗

PubMed1995

[The regulation of deep peroneal nerve inputs on depressor response evoked by caudal ventrolateral medulla excitation in rabbits].

Experiments were performed on 33 white male rabbits anaesthetized with chloralose and urethane under artificial ventilation. The results were as follows: (1) depressor response could be evoked by electric stimulation of caudal ventrolateral medulla (cVLM). The inputs of deep peroneal nerve (DPN) could inhibit partly the depressor response evoked by electric stimulation of cVLM (P < 0.001). The regulation effects of DPN disappeared in 25-30 min. (2) after iv naloxone (1.5 mg/kg) or microinjection of naloxone into rostral ventrolateral medulla (rVLM) (2 micrograms/1 microliter NS), the inhibitory effect of DPN inputs was abolished partly (P < 0.01, P < 0.001). These results suggest that: (1) the depressor response elicited by cVLM excitation in rabbits can be regulated by inputs of DPN; (2) opiate peptide in rVLM may take part in the inhibitory effect of DPN inputs on depressor response excited by cVLM.

Animals↗

PubMed1995

[Mediastinoscopy: value in diagnosis of middle mediastinal masses].

Between 1991 and 1994 standard cervical mediastinoscopy (SCM) was performed in 22 patients with enlarged middle mediastinal nodes of unknown causes. The pathologic diagnoses were achieved in all patients. Malignancies were found in 45.5% of the patients (10/22) and benign lesions in 54.5% (12/22). An erroneous diagnosis and hence inappropriate treatment were avoided. In our experience correct diagnosis can be made by mediastinoscopy in cases of middle mediastinal nodes enlargement, which is usually impossible by routine examination.

Adolescent↗

PubMed1995

[The ultrastructure of intermediate type trophoblast cell].

Transmission electron microscopy was used to clarify the detailed morphology of the "intermediate type" trophoblast cell in normal and tumor issue. 67 normal placental villi specimen, 10 placental bed specimens and 10 malignant mole, 10 hydatidiform mole, 5 choriocarcinoma specimen (the last three types taken before chemotherapy) were examined. Results showed that the transitional type trophoblasts of the placenta were developed from cytotrophoblasts through differentiation and fusion to syncytiotrophoblasts which showed features of maturation and aging, having features of cytotrophoblast nuclei and syncytiotrophoblast cytoplasm. The transitional trophoblast of placental bed showed similar morphology as that of transitional type cells of villi. The morphology of transitional type cells of villi. The morphology of transitional type cells of trophoblastic tumors had both normal morphology and cellular hyperplasia, atypia and features of tumor ultrastructure. The prominent feature was the high electron density of the granules and polymorphic cysts crowded in villi, demonstrating that the morphology of "intermediate type" trophoblasts in placental and tumor tissue are similar, whereas heterotype cellular morphology is present in varying degrees in tumor tissue.

Choriocarcinoma↗