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Biomedical subjects

R Zhang

Publications and source records attributed to R Zhang.

At least 235 records · Page 13Linked to original sources
PubMed1999

Combined effect of alpha-fetoprotein antisense oligodeoxy-nucleotides and 5-fluorouracil on human hepatoma cell growth.

OBJECTIVE: To investigate the effect of alpha-fetoprotein (AFP) antisense phosphorothioate oligodeoxynucleotides in combination with 5-fluorouracil (5-FU) on the growth of BEL-7404 human hepatoma cells in vitro. METHODS: Phosphorothioate oligodeoxynucleotides were synthesized by beta-cyanoethyl-phosphoramidite chemistry. AFP gene expression in human hepatoma cells was determined by avidin-biotin-peroxidase complex (ABC) immunocytochemical method. Cell growth in the presence or absence of experimental agents was measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-dipheny ltetrazolium (MTT) microculture tetrazolium assay. RESULTS: AFP antisense oligomers markedly suppressed the growth of BEL-7404 human hepatoma cells in vitro by sequence-specific blocking of the AFP gene expression in the cells (P < 0.05). 5-FU also inhibited the hepatoma cell growth in a dose-dependent manner when used alone (P < 0.05). The combined treatment with AFP antisense oligomers and 5-FU showed significantly enhanced hepatoma cell growth inhibition than either AFP antisense or 5-FU treated cells alone (P < 0.05). CONCLUSION: Combined use of AFP antisense oligomers and 5-FU could more effectively inhibit the growth of BEL-7404 human hepatoma cells in vitro.

Antimetabolites, Antineoplastic↗
PubMed1999

[Preliminary exploration on TCM literature of Heishuicheng collected in Russia].

The Heishuicheng literature collected in Russia was excavated by the Russian explorer Kotzlov at the remained site of Heishunicheng of Xixia in Inner Mongolia early this century, and carried back and preserved in Petersburg, Russia. This rare treasure was well and secretly hidden in clandestine room for a long period, not known to any other people. The detailed contents and characteristic and appearance of the valuable TCM literatures among them and its academic and research significance are explored here.

China↗
PubMed1999

[Functional expression of human GM-CSF receptor in NIH3T3 cells].

OBJECTIVE: To characterize human GM-CSF receptor(GM-R) reconstituted in NIH3T3 cells. METHODS: Mouse NIH3T3 cells which do not express human GM-R were transfected with cDNAs encoding the alpha and beta subunit of the human GM-R, and then ligand-stimulated proliferative signal transduction and tyrosine phosphorylation of the positive transfectants were examined. RESULTS: The reconstituted functional receptor, GM-R alpha/beta, could mediate cell proliferation and colony formation in soft agar, and induce tyrosine phosphorylation of beta c, Jak2, Shc and Shc-associated P145(SHIP). However, human GM-CSF did not activate mitogenic signal expression of NIH3T3 cells transfected with GM-R alpha alone. CONCLUSIONS: Introduction of both alpha and beta subunit of human GM-R into NIH3T3 cells can result in ligand-dependent cell growth and colony formation via phosphorylation of beta c and activation of Jak2, Shc and SHIP signal transduction pathway.

3T3 Cells↗
PubMed1999

[Deletions and aberrant transcription of p16 and p15 genes in childhood acute lymphoblastic leukemia].

OBJECTIVE: To explore the role of p16(MTS1) and p15(MTS2) genes in the pathogenesis of childhood acute lymphoblastic leukemia(ALL). METHODS: PCR and Southern blot were used to analyse p16 and p15 gene deletions, RT-PCR was used to analyse p15 gene transcription. RESULTS: Exon 1 and 2 of p16 gene deletion was detected in 8 of 21 patients(38.1%), and exon 1 and 2 of p15 gene deletion in 11 of 21 patients(52.3%). Aberrant p15 gene transcription was observed in two cases. CONCLUSION: High frequency of p16 and p15 gene deletions and aberrant transcription may be involved in the pathogenesis of childhood ALL.

Adolescent↗
PubMed1999

[Cleaning cavity operation in treating relapse bacillary cavitary pulmonary tuberculosis].

OBJECTIVE: To study the effective surgical way for serious relapse multidrug resistant bacillary cavitary pulmonary tuberculosis. METHODS: 104 cases of serious relapse multidrug resistant bacillary cavitary pulmonary tuberculosis were cured by cleaning cavity operation from 1981 to 1998. The operation contained resecting the back rib covering the cavity, separating the intercostal tissue, cutting the external wall of the cavity, cleaning the cavity thoroughly, scraping the cavity till exposing the fresh tissue, killing the tuberculosis germs with organic acid, filling the cavity with the intercostal muscle, fixing and suturing the muscles. RESULTS: Among the 104 cases, 103 cases (99.0%) were cured, 101 cases (98.1%) were cured after the first operation, one case (1.0%) became better. Among the 98 cases followed up, the rate of returning to work were 96%, no one died or relapsed. CONCLUSIONS: The design of cleaning cavity operation is reasonable and it is the effective method to treat the relapse bacillary cavitary pulmonary tuberculosis, the rate of cure and sputum conversion are promising.

Adult↗
PubMed1999

[Clinico-pathological characteristics and prognosis of thymoma: an analysis of 159 cases].

OBJECTIVE: To investigate the clinicopathologic features of thymoma and assess prognostic factors. METHODS: Data of 159 patients operated for thymoma were collected. A retrospective analysis was performed, through comparison of survival rate computed by the actuarial method and rate of recurrence and metastasis. RESULTS: (1) The 3-, 5-, and 10-year survival rate was 78.9%, 63.7% and 46.3%, respectively. All patients treated by subtotal thymoma resection with postoperative radiotherapy survived more than 3 years. (2) Patient's age, location and image density of tumor play important role in differential diagnosis. (3) Tumors with epithelial predominance were more frequently seen in late stage than those of other histologic types(P < 0.01). (4) The prognosis of patients whose tumors had incomplete or no capsule was poor as compared to that of stage I tumor (P < 0.05). (5) According to multivariate analysis, the most important prognostic variables included completeness of resection (OR = 2.10) and clinicopathologic stage (OR = 1.73). Myasthenic crisis was the most important factor influencing operative death. Sex, symptoms and histologic classification did not influence prognosis. (6) Recurrence and metastasis occurred in all stages, but more frequent in the later stages of the disease. CONCLUSION: Complete resection helps decrease recurrence of thymoma. Prognosis of patients receiving palliative resection of thymoma may be improved by postoperative radiotherapy.

Adolescent↗
PubMed1999

[Study of recurrence and metastasis after radical resection of carcinoma of the lung].

OBJECTIVE: To study the characteristics of recurrence and metastasis following radical resection of carcinoma of the lung. METHODS: A total of 1,229 cases of radically resected lung cancer was statistically analyzed using STATA software. The survival rate was estimated using life table method. Comparisons of survival in each group were made according to the log rank test. The differences in rate between groups was assessed by chi 2 test. RESULTS: Recurrence rate was found higher in squamous-cell carcinoma than in adenocarcinoma. It was also higher in sleeve resection and partial resection than in other resection modes (P < 0.05). The rate of lymph node metastasis was higher in adenocarcinomas than in squamous-cell carcinoma (P < 0.05). Lung, bone, brain, liver, subcutis, and adrenal glands were the common resorts of metastasis, in decreasing order of frequency. Metastatic rate was the lowest in squamous-cell carcinoma. Active therapy can still improve the survival even when recurrence and metastasis occurred. CONCLUSION: It is recommended to avoid partial resection for the invaded adjacent lobe whenever patient's condition can stand multilobectomy. It is necessary to assure resection margin length when sleeve resection is performed in order to reduce recurrence. Active therapy can improve survival of patients with recurrence and metastasis.

Adenocarcinoma↗
PubMed1999

[Primary esophageal small-cell carcinoma].

OBJECTIVE: To study the clinical biocharacteristics of primary esophageal small-cell carcinoma (PESC) and factors influencing prognosis and to find rational surgical indications and combined therapy. METHODS: To analyse the clinical materials of 47 patients undergone operation with PESC compared with those of patients with esophageal squamous-cell carcinoma(ESCC) or primary esophageal adenocarcinoma (PEAC). RESULTS: The overal resectability, morbidity and 30-day mortality rate of PESC was 93.6%, 17% and 2.1%, respectively, being similar to those of ESCC or PEAC. TNM staging, lymph node metastasis were the major determinants influencing long-term survival. Tumor length, depth of tumor invasion and type of operation had little influence on long-term prognosis. The 5-year survival rate of PESC was 7.5%, which was much lower than that of ESCC and PEAC (P < 0.01). Among the 42 deceased patents, one died of anastomotic leakage and the others died of remote metastasis and recurrence. Adjuvant chemotherapy did no help improve patients long-term survival. CONCLUSION: Campared with ESCC and PEAC, PESC is the most malignent one with early lymphatic and hematogenous matastases and poor prognosis. Lymph node metastasis is the major factor influencing prognosis. Patients in stage 0, I and II a of PESC are indicated for surgical resection, while those in stage II b, III and IV should be managed with non-surgical combined therapy.

Aged↗
PubMed1999

[Efficacy of Chinese recipes of replenishing kidney and activating blood circulation in female rats with osteoporosis induced by ovariectomy].

OBJECTIVE: To investigate the efficacy of Chinese recipe of replenishing Kidney and activating blood circulation (RKABC) in female rats with osteoporosis induced by ovariectomy. METHODS: Fifty female SD rats at 12 months of age were chosen, and were divided into 5 groups; control, model, model plus nylestriol, RKABC (high dose) and RKABC (low dose). The model of osteoporosis was established by ovariectomy in rats for 12 weeks, and then was given different kinds of liquid and administered approximately for 12 weeks. General bone mineral density (GBMD), femur bending strength (FBS), serum-BGP and urine-Hyp/Cr were measured. RESULTS: GMBD, FBS in 3 treatment groups were significantly higher than those in the model group (P < 0.05), but lower than those in the normal control group (P < 0.05); GBMD, FBS in 2 RKABC groups were slightly higher than those in nylestriol's group (P > 0.05). Compared with model group, levels of serum BGP in 2 RKABC groups increased (P < 0.05) and in RKABC (H) treatment group increased almost the same as those in the control group (P > 0.05). In comparison with model group, levels of U-Hyp/Cr in 3 treatment groups decreased (P < 0.05, P < 0.01) but 2 RKABC treatment groups still significantly higher than those in the normal control group (P < 0.05). CONCLUSION: It indicated that RKABC could control osteoporosis in female rats induced by ovariectomy and seems to promote bone formation.

Animals↗
PubMed1999

[Clinical observation on treatment of postcardiotomic complications with Chinese herbal medicine based on syndrome differentiation with angiocardiopathy].

OBJECTIVE: To study the effect of Chinese herbal medicine (CHM) based on Syndrome Differentiation on postcardiotomic complications in patients with angiocardiopathy. METHODS: Aimed at the frequently encountered postcardiotomic complications including fever, cough and expectoration, belching, abdominal distension, palpitation, short breath, etc. CHM treatment was applied in combination with routine western drugs treatment (cardiac tonic, diuretics, vascular dilatator and anticoagulant). RESULTS: Twenty out of 22 patients with protracted fever and irresponsive to multi-antibiotics therapy were cured, the other one with hydrothorax received other therapy and the another one with drug fever was natural cured after stopping medication. Among 23 patients complicated mainly with respiratory symptoms, 17 were cured and 6 improved, among 15 with digestive symptoms, 12 cured and 3 improved, and among 7 with cardiovascular symptoms, 3 cured, 2 improved and 2 ineffective. CONCLUSION: CHM has good effect on postcardiotomic complications, it could improve the functional recovery of heart and lung.

Adolescent↗
PubMed1999

[Association of Gln223Arg variant in leptin receptor gene with metabolic abnormalities and hypertension in type II diabetes mellitus in Shanghai "Han" population].

OBJECTIVE: To investigate the association of Gln223Arg variant in leptin receptor gene with metabolic abnormalities and hypertension in type II diabetes mellitus. METHODS: The genotypes of Gln223Arg variant in leptin receptor gene were determined by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) assay in 359 unrelated subjects of Shanghai "Han" population (including 193 subjects with normal glucose tolerance and 166 type II diabetic patients). The clinical data were also analyzed. The parameters for regional adipose tissue distribution were measured by magnetic resonance imaging (MRI). RESULTS: Significant difference of genotype frequency of Gln223Arg variant in leptin receptor gene was observed between hypertensive and normotensive type II diabetic male patients (P = 0.008). "A" allele was associated with increased systolic blood pressure (P = 0.0026) and diastolic blood pressure (P = 0.0084) in type II diabetic male patients. Logistic regression analysis showed that this gene variant was an independent risk factor of type II diabetic male patients accompanied with hypertension (P = 0.0031), and it was associated with elevation of systolic blood pressure (P = 0.0054). The odds ratio of hypertension in type II diabetic male patients with "A" allele was 2.825 (95% CI 1.418, 5.627). CONCLUSION: Gln223Arg variant in leptin receptor gene is associated with hypertension in type II diabetic male patients, especially with elevation of systolic blood pressure.

Adult↗
PubMed1999

[Effects of different levels of calcium intake on bone of pregnant women].

OBJECTIVE: To research some effects of different levels of calcium intake in the mother's bone mineral density. METHODS: On the basis of insignificantly different among energy and other nutrition intake excepting calcium, 35 volunteers in pregnant 18 weeks healthy women were randomly divided into I. II and III group [calcium intake were (550 +/- 150) mg/d, (900 +/- 150) mg/d and (1,500 +/- 150) mg/d]. The tracked study began from pregnant 20 weeks to postpartum 45 days. All over and different body of BMD (Bone Mineral Density) were assessed by dual-energy X-rays absorptiometry. RESULTS: I group of many location BMD were below that of other groups (P < 0. 01). As compared with the same age women's lumbar spine BMD, I group was only maintained within the rang (85.14 +/- 6.61)%, and osteoporosis in some places of bone were found in some individual. II group was (90.74 +/- 6.53)%. III group was (100.44 +/- 5.19)%. Allover different body's BMD were significantly correlated with the average BMD (r > 0.8, P < 0.1), the most significant correlation was third lumbar spine. Calcium intake of women was positively correlated with different body's BMD (r > 0.5, P < 0.01). CONCLUSION: III group, calcium intake was (30.68 +/- 3.56) mmol/d, pass through period of pregnancy, parturition and short-term breast-feed mother body remain the same BMD, and prevent osteoporosis. Reporting pregnant women calcium nutrition pass through BMD, lumbar spine is good representation.

Absorptiometry, Photon↗
PubMed1999

[Reconstruction and repair after resection of soft tissue sarcoma].

OBJECTIVE: To emphasize the importance of reconstruction and repair after resection of soft tissue sarcoma. METHODS: From November 1990 to November 1996, in 107 cases of soft tissue sarcoma 32 cases had received various reconstructive or reparative operations. Among the 32 cases, 4 cases were primary sarcomas and 28 cases were recurrent sarcomas. In surgical grading, 3 cases were of I B, 3 cases of II A and 26 cases of II B. Radical resection was performed in 13 cases, widen local resection in 17 cases and local excision in 2 cases. After operation, 13 cases received chemotherapy and 7 cases received radiotherapy. Reconstruction of blood vessels was performed in 3 cases, reconstruction of kinetic function in 16 cases, and repair of defect was carried out in 23 cases. RESULTS: Thirty patients were followed up for 4 months to 6 years and 6 months. The clinical results showed that the local control rate of sarcoma was 80%, limb-salvage rate after reconstruction of blood vessels was 100%, the excellent and good rate after reconstruction of kinetic function was 87.5%, and the survival rate of the tissue flap of transplantation and transposition was 96%. CONCLUSION: It was concluded that the reconstruction and repair after resection of soft tissue sarcoma was the extension of operative treatment, and was very important in lowering the recurrence rate and improving the life quality of the sufferings.

Adolescent↗
PubMed1999

[Diagnostic value of ultrasonography in cervical lymph node metastasis].

OBJECTIVE: To research the accuracy of ultrasonography in the diagnosis of cervical lymph node metastasis and the value of that of cervical lymph node occult metastasis. METHOD: Fifty-five patients(61 sides) operated by neck dissection were double-blindedly studied by preoperation palpation and ultrasonography comparing with postoperating pathological examination. RESULT: Fifty-two sides were confirmed to be pathology lymph node metastasis from 61 sides. 40 and 48 sides were checked out by palpation and ultrasonography, respectively. The sensitivity, specificity and accuracy of palpation and ultrasonography were 76.9% and 92.3% (P < 0.05), 77.8% and 88.9%(P > 0.05) and 77.0% and 91.8%(P < 0.05), respectively. Six sides were checked out by ultrasonography (occult metastasis) in 12 sides of palpation negative. CONCLUSION: The accuracy of ultrasonography was prior to that of palpation in examining cervical lymph node metastsis. Ultrasonography could check out about half occult metastasis and should be regarded as a routine examining items in diagnosing cervical lymph node metastasis.

Adult↗
PubMed1999

[Improvements on radical neck dissection].

OBJECTIVE: To improve the surgery of radical neck dissection for a better curative effect. METHOD: Some improvements on incision, path to exploration and manipulation of operation were made in 65 patients with head and neck tumors while radical neck dissection were done. RESULT: The average surgical time was 110 minutes and the average amount of bleeding was 120 ml in the patients when radical neck dissection had been completed. The complications decreased to 1.54%. The surviual rates of 2 and 3 years are 86.2% (56/65) and 81.6 (31/38) respectively. CONCLUSION: With these improvements on radical neck dissection, the curative effect in the near future is improved.

Adolescent↗
PubMed1999

[Determination of triptolide in tripterygium preparations by gradient high performance liquid chromatography].

A gradient elution method was developed for the separation and determination of triptolide in Tripterygium wilfordii Hook preparations by high performance liquid chromatography (HPLC) using YWG column made in China. Methanol with 0.05 mol/L KH2PO4 was used as mobile phase, and the photodiode array detector was used at lambda = 218 nm. The sample of tripterygium oral preparation was extracted with ethanol and then by chloroform. The extract solution was evaporated and the residue was dissolved in 10 mL of ethanol as a sample solution. The spectrum was obtained by the subtraction of spectrum of ethanol from that of sample solution. In comparing with isocratic elution, the gradient operation used in this paper had higher resolution and sensitivity. The calibration curve was linear over the range of 1.32-21.10 mg/L (r = 0.9999). The average recovery of the method was 97.59%. The method was applied to separate and determine the triptolide in tripterygium tablets and the results indicated that the triptolide content by this method was less than that by isocratic and label-claimed one.

Chromatography, High Pressure Liquid↗
PubMed1999

[Cloning of glycoprotein B gene from strain Rispens of Marek's disease virus and construction of recombinant fowlpox virus].

Purified DNAs from Chicken Embryo Fibroblast (CEF) cultures infected with MDV strain Rispens were used as templates. Specific fragment with the size of about 2.9 kb was successfully amplified through Polymerase Chain Reaction(PCR) and identified to be gB gene of MDV by dot blot hybridization with a digoxigenin-labelled MDV gB specific oligonucleotide probe. The gB gene from strain Rispens was cloned into pUC19 and FPV insertion vector pFG1175-1 to construct plasmid pMGB and pFGBR1775-1 respectively. DOSPER liposome-mediated transfection with insertion vector DNA pFGBR1175-1 was performed on CEF monolayers infected with FPV 3-4 h earlier. Recombinant FPV was clone purified. Immunofluorescence Assay(IFA) showed that MDV gB gene had been expressed in FPV.

Animals↗