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Biomedical subjects

R Yoshida

Publications and source records attributed to R Yoshida.

At least 253 records · Page 14Linked to original sources
PubMed1976

Nonidentical subunits of protocatechuate 3,4-dioxygenase.

Protocatechuate 3,4-dioxygenase (EC 1.13.11.3) has been reported to have a molecular weight of 700,000 and to consist of eight identical subunits, each containing one atom of ferric iron and a substrate binding site. This subunit has now been found to dissociate further into four smaller subunits of two nonidentical types (alpha2beta2), upon sodium dodecyl sulfate gel electrophoresis. The molecular weights of the alpha and beta subunits were estimated to be 22,500 and 25,000, respectively. Isoelectric focusing of the enzyme in 6 M urea revealed that the isoelectric points of the alpha and beta subunits were 5.2 and 9.5, respectively. Separation of the two subunits was achieved by chromatography on sulfopropyl (SP)-Sephadex in 6 M urea after treatment of the enzyme with 8 M urea at 37 degrees C for 6 h. The NH2-terminal sequence of the alpha subunit was determined to be Pro-Ile-Glu-Leu-Leu-Pro-Glu-Thr-Pro-Ser-Glx-Thr-Ala-Gly and that of the beta subunit, Pro-Ala-Gln-Asp-Asn-Ala-Arg-Phe-Val-Ile-Arg-Asx-Arg-Asx. Phenylalanine was found as the COOH-terminal residue of the alpha subunit. However, the COOH terminus of the beta subunit was not detected by any of three methods employed.

Amino Acid Sequence↗
PubMed1976

Benign desoxycorticosterone-producing adrenal tumor.

A woman with a benign tumor of the left adrenal cortex had a six-year history of hypertension. Serum potassium level and plasma renin activity were low. Plasma aldosterone and cortisol levels were low normal, and plasma desoxycorticosterone (DOC) level was extremely high. Iodine 131-labeled cholesterol accumulated in the tumor in the left upper quadrant of the abdomen. At laparotomy, a benign adrenal tumor was excised; thereafter, the blood pressure and plasma DOC levels returned to normal. We believe that this is the first case of a benign DOC-producing adrenal tumor.

Adrenal Gland Neoplasms↗