Search PubMed⌕ Search

Biomedical subjects

R Yarom

Publications and source records attributed to R Yarom.

At least 91 records · Page 5Linked to original sources
PubMed1976

Electron microscopic x-ray microanalysis of normal and leukemic human lymphocytes.

A comparative study of the elemental content of normal and leukemic cells was undertaken on a few subjects, using electron microscopic x-ray microanalysis. Phosphorus, sulfur, chlorine, calcium, copper, and zinc were detected in intracellular loci. The concentration of some of the above elements appeared to be disease related. In leukemic lymphocytes, the nuclear zinc was significantly lower than that recorded in normal lymphocytes, while the phosphorus was only moderately decreased. This suggests a faulty zinc uptake or binding in leukemic cells. The possible consequences of intracellular zinc deficiency are discussed.

Calcium↗
PubMed1976

Gold tracer studies of muscle regeneration.

Rabbits were injected with soluble gold, and cold injury was inflicted upon their extraocular muscles. Following the typical freeze-thaw damage in which the myofiber basement membrane is preserved, a polymorphonuclear granulocyte exudation occurred. At this stage only a few interstitial cells with long processes contained gold. At one and two days abundant mononuclear cells with gold were observed at the site of injury. In subsequent stages of muscle regeneration the amount of tracer decreased but myoblasts, and even fully formed myotubes, often contained detectable gold. Some data on "in vivo" gold salt kinetics and the specificity of gold localization were utilised in the study of the relation between mononuclear interstitial cells and myoblasts.

Animals↗
PubMed1976

Tongue muscle regeneration, Gold marker studies following cold injury.

Rat's tongues were injured by cold and the reactive processes were observed by light and electron microscopy. Injured animals and untreated controls were injected with aurothiomalate and the cells in tongue muscles which contained gold were observed after increasing injection-sacrifice time intervals. In the very rapid regeneration, gold-containing macrophage-like cells fused with each other and with well preserved parts of myofibers. The selectivity of gold localization suggested, at least morphologically, a macrophage to myoblast development. The possible factors affecting the rate of muscle regeneration have been discussed with special reference to the nature of the injurious stimulus and the type of cellular response.

Animals↗
PubMed1976

Aurothiomalate as an ultrastructural marker. Electron microscopy and x-ray microanalysis of various tissues after in vivo gold injections.

Rabbits and rats were given single injections of aurothiomalate by different routes. The animals were killed at progressive intervals, and sections from various organs were examined by electron microscopic x-ray microanalysis. Ultrastructurally, characteristic material was regularly found in vacuoles, dense and heterogeneous bodies of macrophages, hepatocytes and renal epithelial cells. Occasionally, other mesenchymal cells also contained gold. Histochemical and analytical tests showed that the gold-containing organelles were devoid of acid phosphatase activity. The generalized rapid spread, retention and selectivity of localization after a single small dose make aurothiomalate a useful marker substance for ultrastructural studies.

Acid Phosphatase↗
PubMed1976

Spread of gold injected into the joints of healthy rabbits.

This study shows that after intra-articular injection, aurothiomalate and colloidal gold of small (200 A) particle size were rapidly absorbed from joints while the larger, 300 A, particle size colloidal radioactive gold could not be found outside them. Larger particle size suspensions seem therefore more likely to remain localised in the joint and its lining synovium after intra-articular injection, the systemic absorption from the joint cavity diminishing with increasing particle size. It was also found that the intra-articular injection of small amounts of aurothiomalate, of colloidal gold and of colloidal radioactive gold produces identical degenerative lesions in the lining cells of the proximal convoluted tubules of the kidneys. These lesions were always found, although gold particles were demonstrated only in sampled kidney tissues of the animals injected with the soluble gold preparation whereas no gold could be detected in the tissues of animals injected with colloidal non-radioactive or radioactive gold. Electron microscopic evidence is presented to suggest the possibility that the mitochondria are the "target" organelles of the gold-induced cellular damage. Mitochondrial damage was demonstrated in liver and spleen in addition to the already described kidney damage. The correlation between structure and function of the mitochondrial changes is not clear, and ionic shifts may be both a cause and a result of damage.

Animals↗
PubMed1975

X-ray microanalysis of mast cells in rat's muscle.

Mast cells from rat's tongue muscles, fixed with glutaraldehyde only, were examined with an electron microscopic microanalyzer - the EMMA-4. With the preparatory method used, high emissions of a number of elements were obtained in various intracellular spots. The granules of mast cells were found to contain strictly localized high concentrations of sulphur, calcium and zinc.

Animals↗
PubMed1975

Synovitis of familial mediterranean fever. A histologic and ultrastructural study.

The microscopic and ultrastructural features seen in the synovium of twelve patients affected by the protracted arthritis variety of FMF is described. It would appear that the small vessels of the synovial membrane are the principle target organ in this articular pathology. Intra articular injections of colloidal radioactive gold, or hydrocortisone, do not alter the histologic and ultrastructural appearance of the affected synovia. Neither do these findings change in the regenerated synovia, growing after a surgical synovectomy. The implications of these findings are discussed.

Familial Mediterranean Fever↗
PubMed1975

A simplified method of specimen preparation for X-ray microanalysis of muscle and blood cells.

The effect of various steps in the preparation of muscle and blood cells for electron microscopic X-ray microanalysis, was studied with reference to calcium, zinc and copper levels. It was found that these elements are readily detectable in material fixed in glutaraldehyde and embedded directly in Epon 812. Alcohol dehydration, buffer rinses and post-osmication caused marked depletion of all elements examined.

Animals↗
PubMed1975

Nephrotoxic effect of parenteral and intraarticular gold. Ultrastructure and electron microprobe examination of clinical and experimental material.

The presence of gold in the kidney of a 58-year-old woman was confirmed by use of ultrastructural and microprobe examinations. A series of animal experiments were performed in which small single doses of gold were administered systemically and intraarticularly in rabbits. Both the colloidal suspension and the soluable salt gold sodium thiomalate (Myochrysine) were used. Selective lesions occurred in the proximal convoluted tubules and the mitochondria appeared to be the target organelle. They contained gold after gold sodium thiomalate but not after colloidal gold.

Animals↗