[Proceedings: Polyadenylic acid segments in the ribonucleic acids of Semliki Forest virus].
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Adenine Nucleotides↗
Biomedical subjects
R Wittek
Publications and source records attributed to R Wittek.
Direct identification of recombinant vaccinia virus plaques by PCR.
A fast method for the identification of recombinant vaccinia viruses directly from individual plaques is described. Plaques are picked, resuspended in PBS-A and processed for PCR using two 'universal' primers. The amplified sequences are analyzed by agarose gel electrophoresis. This procedure allows discrimination between spontaneously arising TK-negative mutants, which do not carry the inserted gene, and the desired TK-negative recombinants resulting from insertional inactivation of the TK gene.
Base Sequence↗
Regulation of foreign gene in fowlpox virus by a vaccinia virus promoter.
A vaccinia virus promoter was evaluated for regulation of a foreign gene in fowlpox virus by a transient expression assay. Fowlpox virus-infected quail cells, transfected with plasmid DNA containing chloramphenicol acetyltransferase (CAT) gene ligated to a vaccinia virus promoter, expressed CAT activity. No CAT activity was detected either in uninfected cells or fowlpox virus-infected cells. These results indicated that a heterologous vaccinia virus promoter can regulate expression of a foreign gene in fowlpox virus.
Animals↗