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Biomedical subjects

R Werner

Publications and source records attributed to R Werner.

At least 127 records · Page 7Linked to original sources
PubMed1977

[Mucoepidermoid carcinoma of the cervix uteri].

Among 147 re-examined carcinomas of the uterine cervix from the years 1970 till 1975 eight mucoepidermoid carcinomas were found and described. Furthermore we report on one case of a mucoepidermoid carcinoma in situ. The mucous substances are dyed with PAS and the Alcianblue-PAS reaction. A detailed definition of "minimal mucous appearance" is worked out in order to classify a carcinoma as a mucoepidermoid carcinoma. An increase in the mucous producing tendency of squamous cell carcinomas as well as in the number of mucoepidermoid carcinomas during the last two years is stressed.

Adult↗
PubMed1976

Novel structure at 5'-ends of nascent DNA chains.

Because of their association with protein short nascent DNA chains in Escherichia coli can be separated from other cellular DNA by chromatography on hydroxylapatite. Protein-free DNA chains of less than 500 nucleotides in length are resistant to degradation from the 5'-end by alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] and spleen phosphodiesterase (oligonucleate 3'-nucleotidohydrolase; EC 3.1.4.18). In contrast, DNA chains containing more than 500 nucleotides are degradable. From these results we conclude that short nascent DNA chains are structurally modified at their 5'-ends. The nature of this structure and its possible functions are discussed.

Alkaline Phosphatase↗
PubMed1976

[Specific changes of the uterine cervix due to contraceptives].

A group of 344 cervices, produced by conisation within a period of 5 1/2 years, were examined for specific effects of contraceptives. In half of all women, who used oral contraceptives, specific lesions such as "siebartige polypoide Hyperplasie" (cribriform polypoid hyperplasia) were found. The findings and their etiology and importance are discussed.

Adult↗
PubMed1975

A model for compartmentation of de novo and salvage thymidine nucleotide pools in mammalian cells.

When [3H]thymidine is added to HeLa cells grown in thymidine-free medium it is incorporated into DNA almost immediately at full specific activity, blocking any further incorporation of de novo synthesized thymidine nucleotides. Apparently, de novo nucleotide pools [Baumunk, C.N. & Friedman, D.L. (1971) Cancer Res. 31, 1930--35] and salvage nucleotide pools are compartmentalized in HeLa cells. After one generation in the presence of thymidine, the cells begin utilizing nucleotides from both pools to about equal extent.

DNA↗