The biosynthesis of thiamine. A yeast enzyme system which converts 2-methyl-4-amino-5-aminomethylpyrimidine to 2-methyl-4-amino-5-hydroxymethylpyrimidine.
Explore the source record for details and available documents.
Bacillus↗
Biomedical subjects
R Wei
Publications and source records attributed to R Wei.
Preparation of beating heart cells from adult rats.
Suspensions of cardiac cells have been prepared from fragments of hearts from adult rats by prolonged incubation with trypsin and collagenase. In these preparations, about 50 percent of the viable cells routinely exhibit spontaneous contractions ranging from 20 to 120 beats per minute at room temperature. The contractile activity is essentially lost at 2 degrees C but returns to normal as the temperature is increased. The rate of cellular contractions and the percentage of total cells exhibiting contractility are influenced by experimental conditions of cell preparation and the composition of the suspending medium.
Animals↗
The biosynthesis of thiamine. Conversion of 2-methyl-4-amino-5-formylpyrimidine to 2-methyl-4-amino-5-hydroyxmethylpyrimidine by cell-free extracts of baker's yeast.
Explore the source record for details and available documents.
Biological Assay↗
Microassay of thiamine and its phosphate esters after separation by paper chromatography.
Explore the source record for details and available documents.
Chromatography, Paper↗
An open-label, randomized, controlled, 4-week comparative clinical trial of barnidipine hydrochloride, a calcium-channel blocker, and benazepril, an angiotensin-converting enzyme inhibitor, in Chinese patients with renal parenchymal hypertension.
This study compared barnidipine, a calcium-channel blocker, and benazepril, an angiotensin-converting enzyme inhibitor, in 85 Chinese patients with renal parenchymal hypertension (diastolic blood pressure range 95 - 110 mmHg). Patients were randomly assigned to receive either 10 mg barnidipine or 10 mg benazepril orally daily for 4 weeks. In patients with diastolic blood pressure > 90 mmHg after 2 weeks of treatment, the dose of barnidipine or benazepril was increased by 5 or 10 mg, respectively. Both the barnidipine-treated group (n = 43) and the benazepril-treated group (n = 42) showed significant mean reductions from baseline in sitting systolic and diastolic blood pressures. The decrease in diastolic blood pressure with benazepril was significantly greater than with barnidipine treatment. Sitting heart rate was not changed by either drug. There was no significant difference in adverse events between the two groups. Barnidipine is similar to benazepril for the treatment of renal parenchymal hypertension.
Adolescent↗
Structural determination of the conjugated metabolites of ritodrine.
Ritodrine is a beta-2 adrenergic agonist which is used clinically for the management of preterm labor. Since ritodrine is resistant to the action of monoamine oxidase and catecholamine-O-methyltransferase, conjugation is a major route of metabolism. Glucuronide and sulfate conjugates of ritodrine are found in maternal urine. However, the structure of these metabolites has not been determined. The purpose of this study was to determine the structure of these conjugates. Urine from patients on ritodrine therapy was purified by QAE Sephadex ion exchange chromatography. The partially purified conjugates were derivatized and analyzed by GC/MS. The data did not indicate an exclusive site of conjugation. Analysis of both the glucuronide and sulfate conjugates indicates that either of the two phenolic hydroxyl groups may be involved in the formation of conjugated metabolites. However, conjugation of the [2-(p-hydroxyphenyl)-2-hydroxy-1-methylethyl]amine phenolic hydroxyl is more prevalent for both conjugates. This phenolic hydroxyl group is unique since it is located on the portion of the ritodrine molecule which more closely resembles the structure of endogenous catecholamines.
Biotransformation↗