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Biomedical subjects

R W Stoddart

Publications and source records attributed to R W Stoddart.

78 records · Page 5Linked to original sources
PubMed1967

Pectic polysaccharides of growing plant tissues.

1. The polysaccharide compositions of the cell walls of sycamore cambium and sycamore callus tissue have been analysed and found to be directly comparable. 2. Electrophoretic analyses of the whole pectins prepared from actively growing callus and cambial tissue have shown that these preparations contain, in addition to the neutral and weakly acidic components present in apple fruit, a strongly acidic polygalacturonic acid component. 3. The weakly acidic component of all the pectins was directly comparable with that of the pectinic acid of apple fruit. 4. The components of the whole pectin of sycamore callus tissue have been partially purified and analysed. The neutral and weakly acidic components also found in apple fruit were isolated. 5. The pattern of the composition of the neutral sugars present in the pectins of actively growing tissues of cambium and callus has been compared with those present in apple-fruit pectinic acid. 6. The presence of rhamnose linked as galacturonosyl-(1-->2)-rhamnose has been found in sycamore whole pectin. 7. The difference in the pectins of callus, cambium and fruit appears not to be that of species difference but is more characteristic of the nature of the growth and growth conditions of the cells. This is discussed in relation to the problems of the control and mechanism of plant-cell growth and differentiation.

Arabinose↗
PubMed1967

Metabolic relationships of the isolated fractions of the pectic substances of actively growing sycamore cells.

1. d-Glucose and l-arabinose serve as precursors of the pectic polysaccharides of sycamore suspension-callus tissue. 2. The rates and characteristics of the incorporation of radioactive sucrose, glucose and mesoinositol by sycamore callus tissue have been compared and shown to be different. 3. The time-course of the incorporation of radioactive glucose into the major fractions within the cells has been determined. Approx. 7-10% of the radioactivity incorporated is present in the whole pectin of the cells. 4. A study of the continuous incorporation of radioactive glucose showed that the neutral arabinan-galactan fraction of the pectin quickly became saturated with the radioactive label. During the incorporation of radioactivity from a pulse of radioactive glucose the neutral fraction became progressively less labelled, with a corresponding increase in the radioactivity of the weakly acidic pectinic acid, which is known to contain neutral sugars. 5. When the cells were exposed to a pulse of radioactive l-arabinose, the label accumulated first in the neutral fraction and then after 4hr. it passed to the weakly acidic pectinic acid with a corresponding decrease in the radioactivity of the neutral fraction. 6. The product that was initially labelled during the first hour of exposure of the cells in the stationary phase to radioactive glucose was identified as an incompletely methylated galacturonan in which the radioactivity was present in the anhydrogalacturonide residues. This polysaccharide probably acts as the precursor of the polyuronide portions of both the strongly acidic and weakly acidic pectinic acids. 7. The observations are discussed in relation to the structure of the pectic substances and their function in cell growth and development. A tentative model for their metabolic relationship is put forward.

Arabinose↗
PubMedDate not supplied

Localisation of glycans in the placenta: a comparative study of epitheliochorial, endotheliochorial, and haemomonochorial placentation.

Specimens of mid-term (horse), near-term (pig, cow, sheep, mink) and term (human) placentae and associated tissues have been examined with a panel of 15 biotinylated lectins combined with an avidin-peroxidase revealing system. The aim of this study has been to analyse the expression of glycans at the materno-fetal interface in order to establish whether the morphological diversity exhibited by these six species is reflected by accompanying biochemical diversity, or whether similar types of glycan are expressed in tissues performing similar functions. Lectin staining intensity was scored in the following elements of the interhaemal placental barrier: maternal capillaries, maternal uterine epithelium, the materno-fetal interdigitating microvillous membrane (brush border in the human), trophoblast, and fetal capillaries. A high degree of biochemical diversity was found in the glycan expression of the various placental components within and among placental types. Each layer showed widely differing patterns of lectin binding between species, with only a few findings in common: 1) the relative lack of simple fucosyl termini, 2) the presence of non-bisected bi/tri-antennary N-glycan in most layers, 3) an abundance of terminal N-acetyl galactosamine, and 4) the restriction of high mannose glycans to intracellular granules. This diversity may be a mechanism to avoid hybridisation, although glycan patterns may change between conception and placental development, or it may have evolved as a consequence of morphological changes. It is possible that it may also be part of the cause, rather than the result, of the structural diversity that is so characteristic of mammalian placentation.

Animals↗
PubMedDate not supplied

Lectin histochemistry of cerebral microvessels in ageing, Alzheimer's disease and Down's syndrome.

A panel of 14 lectins was used to investigate the expression of saccharides by cerebral microvessels (MBV) in ageing, Alzheimer's disease (AD) and Down's syndrome (DS). Broad increases in lectin binding with age may reflect changes in amount and diversity of glycoproteins due to the thickening of the basement membrane (BM) common in older persons. In AD, and in persons over 50 years of age with DS, binding of e-PHA, 1-PHA and PAA was increased beyond that of age alone, as was that of UEA-I and BSA-1B4 in AD, but not in DS. Persons under 50 years of age with DS showed no changes inappropriate to their age. These specific increases in AD and DS may reflect selective disease-related changes in BM and could indicate an impaired blood-brain barrier (bbb) function or integrity. However, because they occur (in DS) after the deposition of amyloid (A4) protein and onset of neurofibrillary degeneration, it is unlikely they induce plaque and tangle formation. Such changes in MBV could stem from the loss of neurones from locus caeruleus, raphe and nucleus basalis (which are thought to innervate MBV and exert control over blood flow and permeability) that occurs in DS after 50 years of age.

Adolescent↗