Biomedical subjects
Publications and source records attributed to R W Moore.
Partial derivatives of hyocholic acid were formed under the usual conditions for trifluoroacetylation of bile acids (trifluoroacetic anhydride, 35 degrees C for 20 min). Complete trifluoroacetylation of hyocholic acid was achieved at 80 degrees C for 30 min, or at 60 degrees C for 30 min when a trace of pyridine was added to the reaction mixture.
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Methods are described for the preparation of complement-fixation (CF) and hemagglutination (HA) antigens from the Texas turkey ornithosis agent grown in McCoy cell culture monolayers. The particulate antigens prepared for this study were satisfactory for testing mammalian sera by direct CF tests and avian sera by indirect CF or modified direct CF tests. Comparison of titers were made on human, bovine and ovine sera using direct CF tests employing antigen prepared for this study, 6 BC yolk sac antigen, and a commercially available antigen. The HA antigen agglutinated mouse erythrocytes, but it was not of value in hemagglutination inhibition tests because of "nonspecific" inhibitors in both mammalian and avian sera.
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Infants 3 weeks, 8 weeks, and 14 weeks old were presented with checkerboard patterns which differed in the number of squares they contained. The older the infants, the more complex was the checkerboard that they looked at longest. This result does not appear to depend on changes in visual acuity or accommodation related to age.
Graber, C. D. (Baylor University College of Medicine, Houston, Tex.). R. W. Moore, M. Suzuki, W. E. Redmond, R. M. O'Neal, and B. M. Lockhart. Autochthonous intestinal bacterial flora and cholesterol levels in specific pathogen-free swine fed high-lipid and high-sucrose diets. J. Bacteriol. 92:1290-1297. 1966.-Thirty-two specific pathogen-free (SPF) and conventional swine fed high fat, high sugar, and a standard ration were cultured for intestinal flora, and their blood cholesterol levels were measured. The diets, whether sterilized or not sterilized, fed ad libitum or restricted, did not alter bacterial flora greatly or influence blood cholesterol levels. Anaerobes outnumbered aerobes by several logs. Four autochthonous bacteria, lactobacilli, Escherichia coli, enterococci, and gram-variable, nonspore-forming anaerobes (GVNSA; a type of bacteroides), were shown to be constantly present in all animals regardless of dietary conditions. From the duodenum and jejunum of 14 pigs, GVNSA and Bacteroides nigrescens were cultured in rather large numbers, a finding not previously reported in swine or in most other mammals. This finding may have special significance in reference to bile acid and cholesterol metabolism.
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