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R W Besdine

Publications and source records attributed to R W Besdine.

27 records · Page 2Linked to original sources
PubMed1968

Preparation and description of high-molecular-weight soluble surface antigens from a group A Streptococcus.

High-molecular-weight proteins having M protein reactivity were isolated without acid or alkaline digestion. Treatment of a heat-killed group A Streptococcus with sonic vibration released antigens which reacted strongly and specifically with absorbed type-specific antiserum. This antigen preparation was released without diminishing the total yield of acid-extractable M protein of the original heat-killed cells. Fractionation of the sonic preparation on a sucrose gradient yielded four peaks of M reactivity. When these fractions were placed on Sephadex G-200 columns, the M reactive material of three fractions appeared in the void volumes, suggesting that the active material in each had a molecular weight greater than 300,000. The reactivity of the fourth fraction followed closely the void volume of Sephadex G-100. Chemical analysis revealed heterogeneity of the fractions. Spectral analysis showed virtual absence of nucleic acid in three of the fractions and a moderate amount in the fourth. Bactericidal inhibition tests showed activity of three of the four fractions. Analysis of the fractions by Ouchterlony double-diffusion technique revealed that each of the four fractions had several antigenic constituents. All four contained M antigen. T antigen and a third unnamed antigen were present in some of the fractions. Group reactivity was present in all fractions, but did not reside on the M molecule. The enhanced potential of sonically released antigens to induce high-titer specific precipitating antibodies to M protein is discussed.

Antigens↗
PubMedDate not supplied

Restructuring Medicare for the next century: what will beneficiaries really need?

Medicare coverage falls short of its original mandate of access to modern medicine and protection against the high costs of medical care. These shortfalls destabilize both health outcomes and the economic viability of older adults and their families. Our proposed revisions would promote, rather than discourage, optimal care for beneficiaries. By replacing incentives for fragmented, episodic care with an orientation toward functional status, care management, and integration with long-term care, we can make an invaluable investment in a successfully aging society.

Aged↗