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Biomedical subjects

R Vettor

Publications and source records attributed to R Vettor.

78 records · Page 5Linked to original sources

[The homeopathy problem in contemporary medicine].

The aim of this study is to explain homeopathy, and make a critical evaluation of the doctrine and its clinical practice. The discipline of homeopathy, a medical doctrine advanced by Samuel Hahnemann in the late eighteenth and the early nineteenth centuries, is based on a physiological theory according to which a "vital force dominates the human body in an unopposed and dynamic way"; disease processes are considered the consequence of a disturbance of this vital force. According to this doctrine, there are only three diseases: psora, sycosis and lues. Drugs are believed to act according to the principle of similitude, their efficacy increasing with their progressive dilution. Several schools of homeopathy have emerged since Hahnemann's death. Although they differ from each other, they share two of the fundamental principles advanced by the founder. Homeopathic clinical teaching aims at identifying the greatest number of signs present in the patient and then, on the basis of these signs, and irrespective of any diagnosis, the homeopathic specialist decides what drug(s) should be administered. Homeopathy is a doctrine that can be rationally criticized from three standpoints. First, its content contrasts radically with current scientific knowledge of chemistry, pharmacology, and pathology. Second, despite the fact that homeopathic specialists claim many therapeutic successes, the small number of rigorous studies conducted have not as yet provided convincing evidence that homeopathic treatment is effective against particular disease processes. Third, from a methodological standpoint, homeopathy has a number of serious flaws: above all, it violates both the principle of falsifiability enunciated by Karl Popper as a criterion for the demarcation between science and pseudo-science, and the principle of operative definition. Homeopathy cannot therefore be considered a scientific discipline.

History, 18th Century↗

[The logic of differential diagnosis].

The methodological aspects of making a differential diagnosis have so far received little attention. The aim of the present study is therefore to analyze the logical structure of this phase of clinical procedure. It is commonly believed that making a differential diagnosis involves an inferential process through which the clinician first makes diagnostic hypotheses, rules out all these prospective hypotheses except for one, and then concludes that the one remaining hypothesis identifies the disease affecting the patient. Some authoritative scientific methodologists sustain that the logical mechanism leading to the rejection of a diagnostic hypothesis is the negation of the consequent, and is thus the falsification proposed by Popper as the fundamental inference in experimental science. In the present study it is therefore suggested that in making a differential diagnosis, clinicians utilize disjoined syllogism rather than a negation of the consequent. A description is given of the structures of the two types of disjoined syllogism, which are inclusive and exclusive. An in-depth analysis is made of the entire diagnostic-differential reasoning process, and the authors suggest that, on a logical plane, it consists of two distinct sequential inferences, one being an eliminative induction and the other an inclusive disjoined syllogism. Lastly, it is pointed out that, as in the clinic, perfect pathognomonic signs are rarely encountered, each inference leading to a rejection of a diagnostic hypothesis can never be apodictic, and can only lead to a probable conclusion. The confutation of diagnostic hypotheses thus appears to be based on the rule of the denial of inductive logic.

Diagnosis, Differential↗

Increased muscular phospholipase A2 activity in diabetic rats.

Anomalies in prostaglandin (PG) synthesis have been suggested in both experimental and human diabetes mellitus; increased levels of plasma and tissue eicosanoids has been recently reported by several investigators. One step in prostaglandin synthesis is the enzymatic hydrolysis of membrane phospholipids by Phospholipase A2 (PLA2). Nevertheless the alternative pathway involving Phospholipase C must be considered. An evaluation of PLA2 activity is therefore a useful method for studying prostaglandin synthesis in the peripheral target tissues of insulin activity. We studied PLA2 activity in normal and diabetic rat muscle. Streptozotocin-induced diabetic rats showed significantly higher muscular PLA2 activity when compared with controls (3.04 x 10(-2) +/- 0.50 x 10(-2) versus 1.34 x 10(-2) +/- 0.35 x 10(-2) arachidonic acid pMol.mg protein-1.min-1 (p less than 0.01). This effect was not observed in diabetic animals successfully treated with insulin (1.78 x 10(-2) +/- 0.5 x 10(-2) versus 1.34 x 10(-2) +/- 0.35 x 10(-2) arachidonic acid pMol.mg protein-1.min-1), and a significant correlation was found between blood glucose and muscular PLA2 activity (r = 0.42; p less than 0.05). Our results clearly show that in streptozotocin-induced diabetic rats muscular PLA2 activity is significantly higher. The relationship between blood glucose levels and muscular PLA2 activity and the decrease of PLA2 activity after insulin treatment suggest that these changes may be related to a defect in insulin effect.

Animals↗

[Postprandial thermogenesis and obesity: effects of glucose and fructose].

In order to check whether reduced postprandial thermogenesis, as found in obese subjects depends on insulin resistance, the study tested whether the thermogenetic response to glucose in a group of obese subjects and a group of normal weight subjects differed from that obtained using an insulin-independent monosaccharide such as fructose. Nine obese subjects and 6 control subjects were included in the study. An oral glucose tolerance and fructose tolerance test (75 g) was performed in all subjects on different days. Energy expenditure was calculated both in basal conditions and during the test (resting metabolic rate: RMR) using indirect calorimetry expressed per kg of lean weight, as assessed using bioimpedance measurement techniques. Blood samples were collected to assay glycemia and insulinemia. Results show that increased RMR induced by glucose was significantly reduced in the group of obese subjects compared to controls. In the same group of obese subjects, RMR was found to be significantly higher following fructose in comparison to the glucose response but did not differ from that in controls. Data confirm the existence of reduced thermogenesis in obese subjects induced by glucose. The fact that this phenomenon was not recorded in the same subjects following the fructose tolerance test, whose metabolism is insulin-independent, supports the hypothesis that reduced glucose-induced thermogenesis in obese subjects may depend on insulin resistance.

Adult↗

[Analgesic effect of Sandostatin (SMS 201-995) in acromegaly headache].

Somatostatin analogue (Sandostatin; SMS 201-995) is utilized as a therapy in acromegaly because of its efficiency in inhibiting GH secretion; it induces some clinical improvements, such as headache remission in acromegalic patient, which seem to be unrelated to Gh normalization. We have examined 8 acromegalic patients, suffering from headache, after injection of saline solution and subsequently of SMS 201-995 (100 y), in order to study the mechanism of analgesic effect induced by Sandostatin administration. Headache, by autovaluation test, heart rate frequency, PAO, sistolic and diastolic blood velocity in medial cerebral artery, by utilizing Transcranial Doppler Sonography (SDSV), have been measured before and after saline and after SMS 201-995. GH and beta-endorphin have been also assayed in plasma samples. All patients have shown a rapid and complete improvement in headache after Sandostatin administration. At the same time we have observed an increase in SDSV and a parallel slight increase in PAO values, more evident in the diastolic phase. Plasma beta-endorphin assay has shown rather conflicting results after SMS 201-995 administration. Our results confirm an important and rapid analgesis effect of Sandostatin on acromegaly headache unrelated to GH normalization. The cerebral emodinamic changes suggest their involvement in Sandostatin induced analgesia.

Acromegaly↗

Clinical, laboratory and immunogenetic aspects of arthritis associated with chronic lymphocytic thyroiditis.

OBJECTIVE: Synovial fluid effusions and/or arthritis may be found in patients with chronic lymphocytic thyroiditis (CLT), even in the absence of hypothyroidism. Sometimes these arthropathies can be attributed to the rheumatic diseases frequently associated with CLT, but in some instances the arthritis seems to be independent of any of the diseases known to cause arthritis, and therefore remains unclassified. This study was carried out in an attempt to characterize the type and outcome of arthritis associated with CLT. METHODS: We performed a prospective study with a follow-up of 6.42 years (range 4-13) on 33 patients affected with CLT and presenting with arthritis. All conditions known to cause arthritis were previously excluded. Investigations included HLA typing, x-ray of the affected joints and, when possible, synovial fluid (SF) analysis with an interleukin (IL)-1 beta determination. Patients were divided based on their clinical presentation into two groups: those with polyarthritis and those with oligoarthritis. RESULTS: During the follow-up, 8 out of 16 patients with polyarthritis developed severe rheumatoid arthritis, characterized by bone erosions, high levels of SF IL-1 beta and an increased frequency of HLA DR4. The other 8 patients had polyarthritis in a mild, non-erosive form, which responded well to symptomatic drugs. Oligoarthritis, found in 17 patients, also showed a mild evolution, with frequent spontaneous remissions. The non-rheumatoid polyarthritis and oligoarthritis patients were characterised by the absence of bone erosions, low levels of SF IL-1 beta and an increased frequency of HLA DR3. CONCLUSIONS: We conclude that it is possible to find in association with CLT a type of inflammatory arthritis characterized by a mild, non-erosive evolution, low SF levels of IL-1 beta and an increased frequency of HLA-DR3. This arthritis seems to be independent of thyroid dysfunction and shows a clinical pattern similar to the arthritis usually found in connective tissue diseases.

Adult↗