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Biomedical subjects

R Tomaszewski

Publications and source records attributed to R Tomaszewski.

At least 19 recordsLinked to original sources
PubMed2000

Diagnosing and treatment of skin filariases based on own observations.

7 cases of Mansonella perstans filariasis and 9 of Loa loa invasion were diagnosed and treated in the clinic of the Institute of Maritime and Tropical Medicine in Gdynia in the years 1993-1999. The study group comprised 7 women aged 15-45 and 19 men aged 32-49. The most frequently occurring symptoms and signs of the disease, results of laboratory tests, diagnostic methods, ways of treatment and their efficacy were discussed. The diagnosis was made on the basis of the presence of filaria larvae in peripheral blood and, in part, on positive serological reactions. The most frequently reported and observed in our patients were Calabar swellings, allergic lesions of the skin and general symptoms. In the laboratory tests the most characteristic were elevated white blood cells counts with marked eosinophilia and a rise in immunoglobulin E during the active phase of the disease. In doubtful cases, Mazzotti's test was applied. In spite of the treatment with DEC lasting for 3-4 weeks and a combined therapy with levamisole and albendazole, relapses occurred in 5 cases in the period from 2 months to 2 years after the completion of the therapy. In 3 patients treated with ivermectin the period of observation was still too short (2 and 5 months) to draw conclusions as to the efficacy of such treatment.

Adolescent↗
PubMed1998

Both the 5S rRNA gene and the AT-rich flanks of xenopus laevis oocyte-type 5S rDNA repeat are required for histone H1-dependent repression of transcription of pol III-type genes in in vitro reconstituted chromatin.

Incorporation of somatic histone H1 into chromatin during embryogenesis of Xenopus laevis results in repression of transcription of the oocyte- but not the somatic-type 5S rRNA genes. We showed earlier that a similar effect of the H1 observed in chromatin reconstituted on circular plasmids in vitro depends on its binding to the AT-rich flanks of the oocyte-type 5S rRNA gene. H1 binding results in stabilization of nucleosomes within the oocyte 5S rDNA repeat comprising the 5S rRNA gene with flanks and in reorganization of chromatin on the entire plasmid DNA. Performing in vitro transcription on reconstituted minichromosome templates carrying the oocyte 5S rRNA gene placed in different arrangements and at different distances from the AT-rich flanks, we now establish that the above effects of H1 observed upon its binding to the AT-rich sequences are absolutely dependent on the presence of the 120 bp oocyte 5S rRNA gene in its native position within the flanks. We also find that with the intact oocyte 5S rDNA repeat, the binding of H1 results in repression of transcription of both pol III- and pol II-type genes and that the transcriptionally inactive chromatin state spreads over a distance of at least a few nucleosomes.

Animals↗
PubMed1997

The AT-rich flanks of the oocyte-type 5S RNA gene of Xenopus laevis act as a strong local signal for histone H1-mediated chromatin reorganization in vitro.

In vivo, histone H1 plays an active role in establishing the transcriptionally repressed chromatin state of the oocyte-type 5S RNA genes in the early stages of Xenopus development. By using fully defined in vitro system of chromatin assembly on plasmids with cloned oocyte- or somatic-type 5S gene repeats we found that the oocyte repeat which comprises a 120 bp oocyte-type 5S RNA gene placed within the few hundred bp long native AT-rich flanks, but not the somatic repeat (a similar 120 bp somatic-type 5S RNA gene placed within native GC-rich flanks) enables histone H1 to realign the nucleosomal core particles densely packed on plasmid DNA. The realignment results in creation of the repeat unit of approximately 240 bp and is achieved through complete removal of several core histone complexes from plasmid template with the oocyte-type repeat. This effect of H1 is independent on the plasmid sequences and seems to be solely due to the presence in the oocyte-repeat of the AT-rich flanks. The effects of H1 are completely suppressed by distamycin A, a drug that specifically recognizes and binds oligo(dA).oligo(dT) runs in DNA. The binding of H1 results in increased protection of DNA sites within the AT-rich oocyte-type 5S repeat. In an in vitro transcription assay performed with reconstituted chromatin templates containing plasmids with the oocyte- or somatic-type repeats only the transcription of the oocyte-type 5S RNA gene was repressed in the presence of physiological concentration of histone H1. These results support the view that the AT-rich flanks of the oocyte-type 5S RNA gene are involved in histone H1-mediated chromatin reorganization that results in the transcriptional repression observed in vivo.

Animals↗
PubMed1997

[Closed intramedullary stabilization of femoral shaft fractures with Eder's nails].

Forty-one femoral shaft fractures were treated with flexible Ender nails which were introduced through the great trochanter without reaming of the medullary canal. There was one case of intraoperative femoral neck fracture. No wound infection or osteomyelitis occurred. Axial and rotational malalignment was always due to technical errors. All fractures united at an average of 12 weeks. Transverse fractures, short oblique and those with "unicortical" comminution proved to be stable after Ender nailing, while cases with "bicortical" comminution had tendency for bone shortening and often required additional procedures.

Adolescent↗
PubMed1996

[Endocarditis lenta--a problematic disease].

4 endocarditis lenta cases with negative blood cultures are reported: 3 patients with risk heart lesions and 1 subject with no predisposing cardiac condition. The mean age of the patients was 53.7 years. Based on the cases reported the following items are discussed: evolution of the clinical syndrome, diagnostic difficulties, problem of negative blood cultures, therapeutic procedure.

Adult↗
PubMed1990

Studies on the risk of ischaemic heart disease in fishermen, seafarers and dockers.

To assess the risk of ischaemic heart disease, the following examinations were conducted in 350 seafarers, 480 deep-sea fishermen and 500 dockers: physical examination, chest X-ray, standard 12-lead electrocardiogram, exercise stress test, echocardiography, laboratory tests. A complete medical history aimed at detection the coronary risk factors was collected from each man. The mean age of persons examined was as follows: 47.6 years - fishermen, 44.8 - seamen, and 42.9 - dockers. It was estimated that the main risk factors found both in fishermen and seamen were cigarette smoking, hypercholesterolemia and overweight. The incidence rates of high serum cholesterol level and overweight in the above occupational groups were higher as compared with the prevalence of these factors in the general male population in Poland. The study data argue for the need for adequate dietary change, habit modification and promotion of recreational physical activities among seafarers.

Adult↗
PubMed1990

The use of submaximal exercise stress testing in early detection of ischaemic heart disease in seamen.

50 seamen aged 19-66 years (mean age 41.5 +/- 10.9), with nonspecific pains in the chest but no changes in the resting ECG, were subjected to the submaximal exercise test. The examinations were carried out at the Clinic of the Institute of Maritime and Tropical Medicine in Gdynia. The positive exercise test was recorded in 42% of persons examined. Observed was an increasing prevalence of the positive test with the growing age and period of employment at sea, especially in the group with the risk factors of the heart ischaemic disease present. Introduction of this test in seafarers, aged over 40 years might be considered; it would help to early diagnose this disease. In the presence of several risk factors of the heart ischeamic disease, the exercise test could be recommended for seafarers as early as at the age 30-40.

Adult↗