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Biomedical subjects

R Terry

Publications and source records attributed to R Terry.

At least 55 records · Page 3Linked to original sources
PubMed1987

Avian sarcoma and leukosis virus pol-endonuclease recognition of the tandem long terminal repeat junction: minimum site required for cleavage is also required for viral growth.

Integration of retroviral DNA is a site-specific reaction involving an endonuclease encoded by the viral pol gene (pol-endo). In vitro the pol-endo from avian sarcoma and leukosis viruses (ASLVs) cleaves both DNA strands near the U5-U3 junction of tandem long terminal repeats (LTR-LTR junction) in single-stranded and replicative form (RF)-I substrates. We have reported previously that the sequences that are required for cleavage of single-stranded substrates by the alpha beta form of the pol-endo differ for the plus and minus strands (G. Duyk, M. Longiaru, D. Cobrinik, R. Kowal, P. deHaseth, A. M. Skalka, and J. Leis, J. Virol. 56:589-599, 1985). This is not the case with RF-I substrates, in which a maximum of 22 base pairs of U5 and 8 base pairs of U3 were required for alpha beta pol-endo cleavage in each strand. Insertion of a palindromic octanucleotide (CATCGATG) at the LTR-LTR junction abolished cleavage in RF-I but not in single-stranded DNA substrates. Deletion of the four nucleotides (TTAA) at the junction prevented cleavage in the plus strand of RF-I DNA, but did not affect cleavage of single-stranded DNA. Furthermore, the alpha beta form of ASLV pol-endo did not recognize heterologous LTR-LTR junction sequences from the reticuloendotheliosis virus or Moloney murine leukemia virus in either substrate form, despite their sequence and structural similarities to the ASLV junction. These results support a role for a sequence-specific interaction between the ASLV pol-endo and the LTR-LTR junction domains that are required for cleavage. By using the infectious Rous sarcoma virus clone pATV8-K, we introduced a set of deletions into the U5 region that would be incorporated into the LTR-LTR junction on viral replication. In the unintegrated provirus, the deletions started 43 base pairs from the LTR-LTR junction and extended various lengths toward the junction. Results of transfection studies with these clones indicated that the U5 sequences that are required for virus production in vivo correspond to those that are required for cleavage of RF-I DNA in vitro.

Alpharetrovirus↗
PubMed1985

Arteriography of the carotid bifurcation: oblique projections.

Atherosclerotic plaques may not be demonstrated adequately by standard anteroposterior (AP)-lateral projections of carotid arteriography. We evaluated selective carotid arteriography in oblique projections for 60 individual carotid arteries. Two neuroradiologists evaluated arteriograms in four separate sessions devoted to AP-lateral views alone and in combination with oblique projections. The lumen surface was characterized as "smooth," "irregular," or "ulcerated." When oblique projections were added to AP-lateral views, there was a significant shift of radiologic diagnosis from smooth to irregular or ulcerated. Addition of oblique projections to standard AP-lateral views increases the radiographic diagnosis of carotid artery lesions that may be the source of emboli.

Angiography↗
PubMed1983

Immunohistochemical localization of carcinoembryonic antigen (CEA), CEA-S, and nonspecific cross-reacting antigen (NCA) in carcinomas of lung.

Antisera to carcinoembryonic antigen (CEA), to a physicochemical subset of CEA, namely CEA-S, and to nonspecific cross-reacting antigen (NCA) were used for the immunohistochemical localization of these antigens in human bronchogenic carcinomas using a triple layer immunoperoxidase technique. The study is based on an analysis of tumors from 130 patients. CEA, CEA-S, and NCA were all identified in the membrane and/or cytoplasm of neoplastic cells, and a good correlation between the antigens was observed in a majority of tumors. The presence or absence of these tumor-associated glycoproteins appeared to be correlated with the histologic type of the tumors, especially in small cell anaplastic carcinoma and adenocarcinoma, and the degree of histologic differentiation of adenocarcinomas correlated positively with these tumor-associated antigens. Data from this group of patients suggest that analysis of tissue CEA at the time of biopsy or surgical resection may facilitate a more objective interpretation of serial plasma CEA assays.

Adenocarcinoma↗
PubMed1983

Surgical treatment of carcinoma of the breast. I. Pathological finding and pattern of relapse.

This is a retrospective review of 476 patients who had mastectomy for carcinoma of the breast during 1971-1980. There is a positive correlation of size of the primary tumor and the incidence of axillary nodal metastasis. Infiltrating ductal and lobular carcinoma had a significantly higher incidence of nodal metastasis (and greater change of having four or more positive nodes) than that of medullary and colloid carcinomas. Colloid carcinoma smaller than 4 cm and the less common histological subtypes (comedo, tubular, papillary carcinomas) rarely metastasizes. At a median follow-up time of 53 months, 23% of patients with infiltrating ductal, lobular, or medullary carcinomas and who did not have nodal metastasis had relapse, while 50% of those with nodal involvement had relapse. Among those who relapsed, 18% initially had only locoregional recurrence, 60% had distant metastasis, and 22% had both types.

Adenocarcinoma, Mucinous↗
PubMed1983

Does weight loss cause the exercise-induced increase in plasma high density lipoproteins?

Studies showing an increase in plasma concentration of high density lipoprotein cholesterol (HDL-C) with moderate exercise have usually rejected the role of body weight change in the HDL-C raising process, ostensibly because the amount of weight lost has been negligible. To investigate HDL-C changes more thoroughly, we followed initially sedentary middle-aged men randomly assigned to either a moderate running (n = 36) or a sedentary control (n = 28) group for one year. Among runners, one-year changes in plasma HDL-C concentrations correlated strongly with their body weight changes (r = - 0.53, P less than 0.001). Curve-fitting procedures and regression analysis suggested that processes associated with weight change produce much of the plasma HDL-C changes induced by moderate exercise and that changes in HDL-C concentration predominantly reflect changes in the reputedly anti-atherogenic HDL2 sub-component. Further, the interaction between weight change and plasma HDL-C concentration was significantly different (P less than 0.001) in exercisers and controls suggesting that the metabolic consequences of exercise-induced weight change are different from the consequences of weight change in the sedentary state.

Adult↗
PubMed1982

Presentation of malignant lymphoma in the rectum.

Because of their rarity, rectal malignant lymphomas are generally included in the group of large intestine lymphomas. A study of eight cases of rectal lymphomas revealed distinctive clinicopathologic correlations. Using the Lukes-Collins classification of malignant lymphomas, the authors interpreted all the cases as B-cell lymphomas, supported by immunologic studies in four cases. Seven cases were classified diffuse follicular center cell (FCC) lymphomas; four as small cleaved FCC; and three as small noncleaved FCC. One case was interpreted as plasmacytoid lymphocytic lymphoma. Monoclonality was demonstrated by immunoperoxidase staining of cytoplasmic immunoglobulin in three cases and by immunologic surface marker studies in another case . The small noncleaved follicular center cell lymphomas presented as localized tumors (clinical Stage I), so-called primary lymphomas. The small cleaved follicular center cell lymphomas, by contrast, were part of a generalized lymphomatous process in three of the four cass with demonstrated involvement of the gastrointestinal tract elsewhere in two cases.

Aged↗
PubMed1982

Abnormal bone and parathyroid histology in carcinoma patients with pseudohyperparathyroidism.

Postmortem bone and parathyroid gland histology in nine hypercalcemic cancer patients without bone metastases was compared to bone and parathyroid histology in ten normocalcemia patients. Parameters of parathyroid function, including serum immunoreactive parathyroid hormone, acid base status, serum phosphate, and nephrogenous cyclic AMP were measured in the hypercalcemic group and compared to normals and to patients with primary hyperparathyroidism. Bone histology in all nine hypercalcemic cancer patients showed increased osteoclastic bone resorption and increased fibrous connective tissue in the bone marrow. Parathyroid glands were of normal size in all nine patients but contained little or no fat, one criterion of parathyroid hyperplasia. In the normocalcemic cancer patients only 2/10 had minimally increased bone resorption while 7/10 had decreased or absent stromal fat in the parathyroid glands. Despite the hyperplastic appearance of the parathyroid glands, serum biochemical parameters in the hypercalcemic cancer patients indicate a state of suppressed parathyroid function suggesting that the osteoclastic bone resorption is related to a humoral substance elaborated by the tumors which is distinct from parathyroid hormone.

Adult↗
PubMed1982

Some questions raised by histologic study of RTOG protocols 75-06 and 77-06 as illustrated by selected samples.

Histologic study of about 700 biopsy specimens from patients with prostate cancers collected by the Radiation Therapy Oncology Group (RTOG) reemphasized the difficulty in accurately predicting biologic behavior of the carcinoma in individual cases. Some well-differentiated carcinomas metastasized to regional lymph nodes. Histologic proof of lymph node metastases may be difficult to detect. Some duct carcinomas do not readily fit a Gleason pattern. A variety of histologic grades may be seen either in a single sample or after the passage of time in the same case. Some prostatic epithelial proliferations may closely simulate carcinomas by routine light microscopy, but are biologically benign. Some of these prostate lesions have histologic similarities to breast lesions.

Humans↗
PubMed1982

Biopsy of peripheral lymph nodes.

This retrospective study reviews 653 patients who had peripheral lymph node biopsies for purposes of diagnosis from 1973 to 1977. Overall, 56 per cent of the nodes had benign lesions, 29 per cent had carcinoma, and 15 per cent had lymphoma. Supraclavicular nodes had the highest incidence of metastatic carcinoma (54%), and inguinal nodes had the highest incidence of reactive hyperplasia or lymphadenitis (71%). Detailed distribution according to histological subtypes, age, and sex of patients are presented. Statistically, age is the most useful factor in estimating the probability of whether the lymphadenopathy is due to benign or malignant process. The chance that lymph adenopathy is due to malignant lymphoma is relatively constant among all age groups (11-16). However, the majority of patients younger than 30 years old had Hodgkin's disease, while older patients were more likely to have non-Hodgkin's lymphomas.

Biopsy↗
PubMed1980

Lymph node biopsy for diagnosis: a statistical study.

This is a retrospective and all inclusive study of 925 patients who had isolated lymph node biopsies for diagnosis from 1973 to 1977. Overall, 60% of the nodes had benign lesions, 28% carcinoma, and 12% lymphoma. The comparable figures for abdominal nodal biopsies were 63%, 33% and 4%; for intrathoracic nodes, 73%, 26% and 1%; for peripheral nodes, 56%, 29% and 15%. Detailed distribution according to specific site of nodal biopsy, histological subtypes, age, and sex of patients are presented. Statistically, age is the most important factor useful in estimating the probability of whether the lymphadenopathy is due to a benign or malignant process.

Abdomen↗
PubMed1980

Epidermoid cysts of testis.

Six cases of epidermoid cyst of the testis are presented. Conservative surgical treatment of this condition is discussed.

Adolescent↗