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Biomedical subjects

R T Huang

Publications and source records attributed to R T Huang.

50 records · Page 3Linked to original sources
PubMedDate not supplied

Labeling of animal cells with fluorescent dansyl cerebroside.

A dansyl (diaminoaphthalenesulfonyl)-derivative of cerebroside was prepared which could be effectively incorporated into the plasma membranes of tissue culture cells and erythrocytes. The cells which had assimilated the glycolipid fluoreced intensely and could be observed under a fluorescent microscope. Cells were initially labeled rather homogeneously over the whole surface. With longer incubation time organization of the fluorescent glycolipid took place and patches of the lipid in the membrane were formed. The redistribution and organization of the membrane lipid could be demonstrated most clearly when cells labeled with this fluorescent glycolipid were infected with myxoviruses. After infection of MDBK and BHK cells with fowl plaque virus areas of dense fluorescence appeared at margines of neighboring cells. When BHK cells were infected with Newcastle disease virus fusion of the cells was accompanied by complete redistribution of the glycolipid. Erythrocytes could also easily incorporate dansyl cerebroside. Chicken erythrocytes which contain cytoplsmic and nuclear membranes incorporated the fluorescent glycolipid in both membranes.

Animals↗
PubMedDate not supplied

Transfer of glycolipid between membranes of tissue culture cells, using dansylcerebroside as a model.

Donor cells, which had incorporated dansylcerebroside in their membranes, could further transfer this glycolipid to monolayers of acceptor cells. The case of transfer varied among acceptor cells, BHK cells being the best and MDBK cells the poorest acceptors of the cells tested. The process of transfer seemed to be mediated by lipids rather than by proteins of the membranes. The mode of attachment between donors and acceptors, such as classified as loose contact, tight adhesion or binding by lectin, did not significantly influence the extent of glycolipid transfer. However, modification of plasma membranes by infection of acceptor cells with myxoviruses resulted in enhancement of glycolipid transfer in some cases. Various factors have been evaluated with respect to dynamics of cellular membranes.

Animals↗
PubMedDate not supplied

Artificial phospholipid and glycolipid particles, visualization of their structures by fluorescence markers and some biological properties expressed by these particles.

Homogeneous large (5--30 micrometer) lipid particles comparable in size and shape to blood cells can be obtained when excess of cholesterol is homogenized with egg phospholipids or brain glycolipids in a Dounce apparatus. The particles contain distinct structures of membranes and lipid cores as visualized by the included fluorescence markers. When these particles were used as models in studying interactions between membranes, it was demonstrated that several properties normally pertaining to natural membranes could be exhibited by lipids alone. It could also be shown that particles containing phospholipids or glycolipids were very different in their adhesiveness to tissue culture cells and in their response to sera of various species.

Agglutination Tests↗