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Biomedical subjects

R T HINEGARDNER

Publications and source records attributed to R T HINEGARDNER.

6 recordsLinked to original sources
PubMed1963

RATIONALE FOR A UNIVERSAL GENETIC CODE.

A mutation in the genetic code would place new amino acids in certain loci and entirely eliminate amino acids from other loci of practically all proteins in an organism. It is reasonable to postulate that mutations of this kind cannot supplant the original code. The genetic code, once established, would therefore remain invariant.

Amino Acids↗
PubMed1962

The isolation of nuclei from eggs and embryos of the sea urchin.

NUCLEI CAN BE ISOLATED FROM THE EGGS AND EMBRYOS OF THE SEA URCHIN BY A FOUR STEP METHOD: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest of the homogenate by centrifugation on a layered sucrose gradient. Contamination of the isolated nuclei is less than 1 per cent. The yield varies between 20 per cent and 80 per cent depending on the developmental stage used.

Animals↗