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Biomedical subjects

R T Glass

Publications and source records attributed to R T Glass.

At least 19 recordsLinked to original sources

Partial spectrum of microorganisms found in dentures and possible disease implications.

While it would appear that denture surfaces alone become colonized by microorganisms, this study showed that the porosity of denture material allows for contamination throughout the entire denture. Further, the numerous opportunistic and pathogenic microorganisms found in this study were unexpected and are known to produce not only substantial oral infections, but also systemic diseases.

Bacterial Infections↗

Comparison of the effectiveness of several denture sanitizing systems: a clinical study.

The purpose of this clinical study was to test the effectiveness of three methods of decontamination on complete dentures. Dentures worn by patients for varying lengths of time were handled aseptically and treated with three different treatment modalities. The dentures were touched and sectioned and then retouched to a variety of microbiological media. The quantity of microbial growth was recorded and predominating microorganisms were identified using standard microbiological techniques. System A was found to consistently decontaminate and sanitize dentures worn by patients. System B and System C showed variable reduction of microorganisms. An unexpected spectrum of both pathogenic and opportunistic microorganisms was found in the dentures examined, including a wide range of gram-negative bacteria, gram-positive bacteria, and yeasts. A wide range of microorganisms must be considered when treating either oral or systemic diseases in denture wearers. Denture hygiene and decontamination are critical to the prevention of oral and systemic disease transmission. The dentures of ill patients must be considered as possible sources of pathogenic microorganisms.

Analysis of Variance↗

Toothbrush care.

Explore the source record for details and available documents.

Equipment Contamination↗

The Oklahoma City bombing: the roles of the dental teams ... and the lessons learned.

The Oklahoma City bombing is considered by most historians the worst act of domestic terrorism ever to have occurred in the United States. The response to the disaster, however, is considered by most experts in the field as being the best; creating what is known as the "Oklahoma Standard". The roles of the dental teams in the resolution were pivotal and until now, the recognition of these roles have not been described, due to this being considered a case of mass murder. The purpose of this paper is to give an overview of the preparations, the initial responses, the roles of the dental teams in the resolution, the preparations for the criminal trials, and some of the lessons that were learned.

Crime Victims↗

Microorganism presence in desquamative gingivitis.

Histologic tissue analysis was retrospectively performed on 19 cases which met the diagnostic criteria of desquamative gingivitis (DG) or benign mucous membrane pemphigoid (BMMP) to determine whether microorganisms were present. Original paraffin blocks were resectioned and tissue specimens were stained with hematoxylin and eosin and with Brown and Brenn solutions. The presence of microorganisms at specific sites was recorded and identification of gram type and histomorphology was made. Bacteria were seen in hematoxylin and eosin and Brown and Brenn stained specimens on the epithelial surface in 31.6% and 68.4% of cases, within the epithelium in 0% and 57.9% of cases, within the subbasilar cleft in 52.6% and 94.7% of cases, and within the submucosa in 5.3% and 57.9% of cases respectively. The epithelial surface was colonized primarily by gram positive cocci (47.4%) and gram negative bacilli (42.1%), which also were the most common types of bacteria seen within the epithelium (26.3% each). 94.7% of specimens examined demonstrated gram negative bacilli within the subepithelial cleft. The predominate form of bacteria found in the submucosa was gram negative cocci (42.1%). Results of this study show that colonization of desquamative gingivitis/benign mucous membrane pemphigoid tissue samples occurs.

Adult↗

The expert witness.

More and more, dentists are being asked to be involved in medical-legal proceedings. The criteria for being an expert is based upon education, training, and experience, all of which every licensed practicing dentist possesses. Dental record-keeping and unbiased, thorough case analysis are the important factor involved in medical-legal litigation.

Expert Testimony↗

Acute encephalopathy induced in cats with a stealth virus isolated from a patient with chronic fatigue syndrome.

A simian cytomegalovirus-related stealth virus, isolated from a patient with the chronic fatigue syndrome, induced an acute neurological illness when inoculated into cats. Histological examination of brain tissue showed foci of cells with cytoplasmic vacuolization and an absence of any inflammatory reaction. Electron microscopy confirmed the presence of herpes-like viral particles and viral-like products in the brain of an inoculated animal. These findings support the role of stealth viruses in the pathogenesis of human neurological diseases and provide an animal model to evaluate potential antiviral therapy.

Acute Disease↗

The ingredients of a successful snuff cessation program.

From the brief outline of the details so necessary for a successful snuff cessation program, it is apparent that snuff addiction is a complex and difficult issue. In the same way that some things in clinical dentistry you prefer to do and some things you prefer to refer, so it may be for your snuff addicts. By virtue of your rapport with your patient, you may be the best to treat the patient's addiction or you may want to refer the patient to an established snuff cessation program (not a cigarette cessation program). Simply telling the snuff addict not to dip is grossly inadequate. Dentists have been very successful in stemming the tide of dental caries and periodontal disease. From the knowledge gained in these prevention programs and the fact that snuff has such an impact in the mouth, the dentist is the natural health care provider to conduct successful snuff cessation programs.

Adolescent↗

The effectiveness of a u-v toothbrush sanitizing device in reducing the number of bacteria, yeasts and viruses on toothbrushes.

Sixty-six sterile toothbrushes were exposed to one of the following microorganisms: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillis subtilis, Serratia marcescens and Baker's yeast. The Pollenex DS60 Daily Dental Sanitizer was found to be effective in substantially reducing the number of retained bacteria and yeasts as compared to contaminated toothbrushes not treated with such a device. Different toothbrush types had different response rates. Seventy-two sterile toothbrushes were exposed to Herpes Simplex Virus, Type I and seventy-two sterile toothbrushes were exposed to Parainfluenza Virus, Type III. The Pollenex DS60 Daily Dental Sanitizer consistently killed both viruses on all of the toothbrushes treated. Both viruses were consistently retained on non-treated toothbrushes for at least 24 hours.

Bacillus subtilis↗

The ingredients of a successful snuff cessation program.

From the brief outline of the details so necessary for a successful snuff cessation program, it is apparent that snuff addiction is a complex and difficult issue. In the same way that some things in clinical dentistry you prefer to do and some things you prefer to refer, so it may be for your snuff addicts. By virtue of your rapport with your patient, you may be the best to treat the patient's addiction or you may want to refer the patient to an established snuff cessation program (not a cigarette cessation program). Simply telling the snuff addict not to dip is grossly inadequate. Dentists have been very successful in stemming the tide of dental caries and periodontal disease. From the knowledge gained in these prevention programs and the fact that snuff has such an impact in the mouth, the dentist is the natural health care provider to conduct successful snuff cessation programs.

Dental Caries↗

The dilemma of denture contamination.

Fourteen dentures from patients requiring new dentures were cultured, treated with Clorox/Cascade/H2O solution, Brushgard, Polident, or distilled water and re-cultured. All 14 dentures were contaminated with multiple microorganisms. The Clorox/Cascade/H2O solution was most effective at decontamination with 71 percent of the treated dentures showing no growth of microorganisms. Brushguard showed no growth in 57 percent; Polident showed no growth in 36 percent; and water showed no growth in 14 percent. Of note, Brushgard, Polident, and water-treated dentures also allowed growth and predominance of other microorganisms that did not predominate on the original culture. The results of this study indicate that there is no product on the market at this time capable of consistently disinfecting the denture depths.

Denture Cleansers↗