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Biomedical subjects

R Stupnicki

Publications and source records attributed to R Stupnicki.

At least 37 records · Page 2Linked to original sources
PubMed1984

Blood levels of 15-keto-13, 14-dihydroprostaglandin F(2alpha) during the postpartum period in primiparous cows.

Plasma levels of the PGF(2alpha) metabolite 15-keto-13, 14-dihydroprostaglandin F(2alpha) were determined postpartum in 51 primiparous Black and White Lowland cows. The highest geometric mean was 1702 pmol/l on day 3 and lowest 190 pmol/l on day 21. It should be noted that variation between animals in the concentration of the metabolite is high. For instance, on days 4, 10, 16 and 22, concentration of metabolite ranged from 775-2500, 209-2450, 45-851 and 30-398 pmol/l, respectively. The duration of the massive postpartum release of PGF(2alpha) could be determined in only 29 cows. Significant correlations were found between the duration of elevated PGF(2alpha) metabolite levels and the time required for completion of uterine involution (r = -0.41, P < 0.05) and between the duration of increased PGF(2alpha) metabolite levels and the interval from parturition to occurrence of the first ovulation followed by a normal luteal phase length (r = -0.37, P < 0.05). The occurrence of the first ovulation followed by a normal luteal phase length in the 29 cows was positively correlated with the time needed for completion of uterine involution (r = 0.54, P < 0.01).

Journal Article↗
PubMed1982

Direct radioimmunoassay of progesterone in human plasma.

A direct method has been described which enables a specific assay of progesterone without extraction in human plasma or in other material containing corticosteroid binding globulin (CBG). When using 20 microliter of plasma per tube, a sensitivity of 6 pg/tube, or 0.3 ng/ml can be achieved. The precision and accuracy are comparable to those observed in methods involving extraction. The described method is particularly suitable for routine assays.

Humans↗
PubMed1976

Radioimmunoassay of LH in blood plasma of farm animals.

A radioimmunoassay technique has been developed for measuring LH in blood plasma of cattle, sheep and pigs, by using an antiserum against bovine LH. Either ovine or bovine LH could be used for labelling and calibration curves in case of these two species, while porcine LH was to be taken in case of pigs. Working range of the calibration curve was 0.3 to 20 ng/ml. The between-assay error ranged from 17 to 7.5% between 1 to 5 ng/ml, the within-assay error from 10 to 4%, respectively. A system of quality control of the assay was developed, based on a series of pretested sera by using the chi-square function.

Animals↗
PubMed1976

Binding of testosterone in mouse plasma.

The binding of testosterone (T) in the plasma of adult male mice was examined using florisil absorption. The binding decreased as the temperature of incubation increased, and was substantially reduced by preheating the plasma at 75 degrees C. The addition of an excess of cold T did not displace 3H-T, and the binding did not depend on the concentration of endogenous T in the plasma. It appears that, under physiological conditions, approximately 60% of the T present in the peripheral circulation of male mice is bound to plasma components, probably mostly to albumin.

Animals↗
PubMed1975

The effects of CBG on the in vitro reduction of 3H-progesterone by liver homogenates and cell suspensions.

The effects of CBG on 3H-progesterone metabolism by ovine, bovine and rat liver homogenates and bovine and rat liver cell suspensions were studied. As a source of CBG served plasma from "estrogenized" women. Same plasma, pretreated at 60 degree for 20 min. served as control. It was found that the amounts of 3H-progesterone remaining after incubation were higher in the presence of CBG, as compared with control incubations, when using the homogenates. No such effect was observed in case of cell suspensions.

Animals↗
PubMed1975

Direct radioimmunoassay of progesterone in plasma of farm animals.

A radioimmunoassay technique has been developed for measuring progesterone directly, without extraction, in 20-mul-aliquots of plasma. The assay was performed in disposable polystyrene test-tubes. Working range of the calibration curve was 0.3 to 10 ng/ml, the precision rangin from 30 to 6%, respectively. The results obtained by the presented method are fully comparable to those reported by others. This method has been applied to farm animals, but is also applicable to other species whose plasma has a low affinity for progesterone.

Animals↗
PubMed1975

Plasma levels of LH and prolactin in cows following enucleation of the persistent corpus luteum.

LH and prolactin were measured in plasma of 5 cows before and after enucleation of the persistent corpus luteum. Two cows returned to normal oestrous cycles following enucleation and did not differ from normal, cycling cows, with respect to hormone levels. Three other ones developed follicular cysts following enucleation and had decreased levels of LH and prolaction as compared with cycling cows. On the day of enucleation the fluctuations in LH-levels were higher than either before or after enucleation.

Animals↗
PubMed1975

Peripheral plasma levels of lh in the cow throughout the estrous cycle.

Peripheral plasma levels of LH during the bovine estrous cycle were measured in 5 cows and one heifer by using double antibody radioimmunoassay. The sources of variability of basal LH-levels were analyzed in detail. The range of basal levels of LH WAS 0.3--3.5 Ng/ml. Between days 11 and 14 of the cycle the mean daily levels were lower in all cows by about 28% as compared with the remaining parts of the cycle. This did not seem to be directly influenced by changes in progesterone levels. A single peak of LH was observed around estrus. The magnitude of that preovulatory peak varied from 6.7 to 16.0 mg/ml, and lasted only a few hours.

Analysis of Variance↗