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Biomedical subjects

R Storb

Publications and source records attributed to R Storb.

At least 865 records · Page 48Linked to original sources
PubMed1971

Marrow grafts between canine siblings matched by serotyping and mixed leukocyte culture.

Marrow grafts were carried out between 16 canine sibling donor-recipient pairs. The pairs were matched by serological histocompatibility testing and were nonreactive in a one-way mixed leukocyte culture. Recipients were prepared for transplantation by 1500-1580 R of total body irradiation. Donor marrow was infused within 4 hr of irradiation. Recipients were not given immunosuppressive drug therapy after grafting. All 16 recipients showed evidence of prompt and sustained allogeneic marrow engraftment. Six died between 30 and 128 days after grafting with graft-versus-host disease, and three died between days 72 and 230 with pneumonia but no evidence of graft-versus-host disease with the exception of lymphoid atrophy. Seven recipients survived without graft-versus-host disease and are in excellent health between 200 and 684 after grafting. In summary, fatal graft-versus-host disease was observed in a number of canine recipients despite matching with their sibling donor by serological histocompatibility testing and by mixed leukocyte culture in a manner similar to that employed to define human HL-A matched sibling pairs. The graft-versus-host disease in these matched siblings developed more slowly than that observed in mismatched dogs, but the ultimate death of approximately half of the matched recipients emphasizes the need for posttransplantation immunosuppression even in this "compatible" situation.

Animals↗
PubMed1970

Colony inhibition of fibroblasts from chimeric dogs mediated by the dogs' own lymphocytes and specifically abrogated by their serum.

Nine canine irradiation chimeras were studied between 173 days and 7.5 years after 1200 to 1500 r of total body irradiation and transplantation of allogeneic bone marrow. Skin fibroblasts from chimeras and normal dogs were tested for colony inhibition by exposure to sera and peripheral blood lymphocytes from both chimeric and normal dogs. Lymphocytes from the chimeric dog were found to inhibit colony formation by its "own" fibroblasts while lymphocytes from other chimeras or from normal dogs did not. Serum from the chimera specifically abrogated this inhibitory effect. These results indicate that the immunological "tolerance" of the chimeric dog is mediated in vivo by blocking serum factors.

Animals↗