[An instrument for measuring skinfold thickness. A short report].
The paper describes a new skinfold caliper for body fat evaluation. The operating pressure of the instrument is 10 p/mm2. Results are displayed on a dial.
Adolescent↗
Biomedical subjects
R Stoll
Publications and source records attributed to R Stoll.
[Transient shift in hearing threshold in simultaneous exposure to noise and postural activities].
Experimental results indicate that work posture--conditioned loads in exposure to broadband and narrow-band noise (L = 85 dB [A]) modify the amount of the noise-induced TTS2.
Adult↗
[Kaposi's sarcoma with pericardial tamponade in AIDS].
In two males with AIDS, aged 26 and 39 years, who had disseminated Kaposi's sarcoma, massive pericardial effusion with tamponade (confirmed by echocardiography) developed in the final stages. Multiple pericardial taps, of up to two litres, of serous fluid yielded no malignant cells or pathogens cytologically or microbiologically. At autopsy the finding was of cytomegalovirus and cryptococcus septicaemia, respectively, and generalized Kaposi's sarcoma with infiltration of the heart along the great vessels and subepicardially along the coronary vessels. The pericardial sac was distended balloon-like with 800 ml sanguinous fluid (in both cases). Histological examination did not demonstrate cytomegalic inclusion bodies or fungal granulomas in cardiac tissue. The pericardial effusion was probably due to tumour spread in the lymph channels and along the vasa vasorum.
Acquired Immunodeficiency Syndrome↗
Calmodulin-mediated effects of loperamide on chloride transport by brush border membrane vesicles from human ileum.
We investigated whether the synthetic opiate loperamide-HCl is able to regulate specific transport systems for sodium and chloride in brush border membrane vesicles (BBMVs) from human ileum and whether such activities are mediated by calcium/calmodulin. In BBMVs we studied Na+/H+ antiport, Cl+/OH- antiport, Na+/Cl- cotransport, and the Cl- conductive pathway. Brush border membrane vesicles were incubated with 10 microM loperamide over 4 h at 5 degrees C before the uptake experiments. In ileal BBMVs, loperamide stimulated intravesicular accumulation of Na+ in the presence of Cl- and vice versa. After 1 min of incubation, the stimulatory effect was 35% +/- 5% (p less than 0.005) of the control without loperamide. Loperamide also stimulated Cl-/OH- antiport by 30% +/- 5% (p less than 0.005) in BBMVs of ileum. In addition, we studied the role of Ca2+/calmodulin in the action of loperamide on chloride transport by human BBMVs. In loperamide-pretreated BBMVs, calmodulin activity was significantly decreased (12 +/- 2 vs. 38 +/- 4 pmol/mg protein). When loperamide-pretreated vesicles were incubated with 2 microM calcium (free concentration) plus 5 microM calmodulin for 1 h at 5 degrees C, complete inhibition of the stimulatory effect of loperamide on Cl-/OH- antiport and Na+/Cl- cotransport was observed. Increasing the Ca2+/calmodulin activity of loperamide-pretreated BBMVs with 2 microM calcium plus 5 microM calmodulin led to a significant inhibition of Cl-/OH- antiport and Na+/Cl- cotransport by 40% +/- 5% (p less than 0.005).
Biological Transport↗
[Regression of mullerian ducts in the female chick embryo treated by norethindrone and gonadotropins].
There is no correlation between the antigonadotropic activity of norethindrone and the regression of Mullerian ducts induced by this substance in the female chick embryo.
Animals↗
[Optimizing puncture of the internal jugular vein. Effects and advantages of the Valsalva maneuver in catheterization].
Shortly after the publication by English et al. on catheterization of the internal jugular vein (VJI) in 1969, the first papers reporting complications appeared. To reduce the rate of puncture failures with their consequences and complications, we tried to find the optimal conditions for successful puncture by evaluating serial CT scans. The diameter increase of the VJI was studied in 8 women and 9 men with an average age of about 39 years with 30 mmHg pressure in the upper respiratory passages. The measurement was performed 2 cm below the level of the cricoid cartilage (the probable puncture point for posterior access). It was carried out first during normal respiration, then after forced inspiration and bearing down with the glottis open against a closed system consisting of a manometer. By means of continuous bearing down, a pressure of 30 mmHg was maintained during CT scanning. With this pressure in the upper respiratory passages, the mean increase in diameter of the VJI was 58% on the left side (P less than 0.002) and as much as 78% on the right (P less than 0.001), which is highly significant. In 12 of the 17 test persons (70.6%), the right VJI proved to be the dominant vessel, and the diameter increase achievable by the Valsalva maneuver likewise seems to be greater on the right side than on the left (Table 1). We now recommend a slight Trendelenburg position with the head rotated 45 degrees to the contralateral side. For the first puncture we consider the posterior approach (see Fig. 2) on the right side to be the best.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗
Influence of an antiestrogenic drug (tamoxifen) on Müllerian duct agenesia induced by various steroidal sex hormones in the female chick embryo.
The caudal deficiencies of the Müllerian ducts (MDs) induced in chick embryos after early treatment with testosterone propionate (TP), 17 beta-estradiol benzoate (EB), or dihydrotestosterone (DHT) are the consequence of agenesia, i.e., a stop in duct development occurring during the sexually indifferent stage. The present work shows that EB and DHT act on the MDs in binding cellular estrogen receptors. Indeed, the antiestrogenic drug, tamoxifen, which competes with estrogens at the receptor site level, significantly decreases the percentage and extent of these MD caudal deficiencies. The results also show that such receptors are already present at a time when MDs begin to grow from 4.5 to 5 days of embryonic life onward. On the other hand, tamoxifen does not significantly modify agenesia induced by TP.
Animals↗
Effect of two potent calmodulin antagonists on calcium transport of brush border and basolateral vesicles from human duodenum.
In the present in-vitro study we investigated the possible role of the calmodulin-antagonistic drugs loperamide and calmidazolium in the regulation of transepithelial Ca2+ transport of human duodenum. Brush border membrane vesicles and basolateral membrane vesicles were simultaneously prepared from surgically resected pieces of morphologically intact human duodenum with a modified Percoll-gradient centrifugation method. Brush border and basolateral membrane vesicles were characterized using enzyme marker analysis and electron microscopy: alkaline phosphatase was enriched 20-fold in brush border membrane vesicles, whereas [Na+ + K+]-stimulated adenosine triphosphatase was enriched 15-fold in basolateral membrane vesicles. Calmodulin activity was determined by a specific radioimmunoassay after solubilizing brush border and basolateral membrane vesicles in 1% Triton X-100. In basolateral membrane vesicles, we found no calmodulin activity. In brush border membrane vesicles calmodulin activity was impaired by 50% after pre-incubation with loperamide or calmidazolium. We measured calcium, sodium, D-glucose and D-mannitol uptake with a rapid filtration technique. Before the transport experiments, brush border and basolateral membrane vesicles were pre-incubated with 5 microM loperamide or 5 microM calmidazolium for 60 min at 5 degrees C. In drug-pretreated, brush border membrane vesicles calcium uptake was significantly reduced after 1 min incubation (-25% +/- 5%, P less than 0.05); this effect was completely reversed in the presence of 5 microM calmodulin. In basolateral membrane vesicles, we found two Ca2+ transport systems: (1) Na+/Ca2+ exchange and (2) ATP-dependent Ca2+ transport. In basolateral membrane vesicles loperamide had no effect. Calmidazolium had no effect on Na+/Ca2+ exchange, but significantly inhibited ATP-dependent Ca2+ transport. This effect could not be reversed by calmodulin.
Biological Transport, Active↗
Effect of inhibitors on sodium and chloride transport in brush border vesicles from human jejunum and ileum.
Brush border vesicles were isolated from surgically resected pieces of human jejunum and ileum using a Mg2+/EGTA precipitation method. When compared to the homogenate, the final membrane preparation contained alkaline phosphatase at a 14 times higher concentration and almost no (Na++K+)-stimulated adenosine triphosphatase. An Na+/H+ antiport could be demonstrated in the jejunum by imposing a pH gradient between the interior and the outside of the vesicles (pHinside 5.2, pHoutside 7.2). In the presence of amiloride or harmaline, Na+/H+ antiport was inhibited by 60 +/- 5% (p less than 0.05) or 65 +/- 5% (p less than 0.05), respectively. In vesicles of human ileum we found an Na+/H+ antiport and in contrast to the jejunum a Cl-/OH- antiport could be demonstrated by imposing a pH gradient (pHinside 5.2, pHoutside 7.2). Besides this double-exchange mechanism for sodium and chloride, a Na+/Cl- cotransport and a Cl- conductive pathway could be detected in ileal brush border vesicles. In the presence of the anion transport inhibitors, furosemide, SITS and DIDS activities of Cl-/OH- antiport and Na+/Cl- cotransport were suppressed by 30 +/- 5% (p less than 0.05), 35 +/- 5% (p less than 0.05) and 40 +/- 5% (p less than 0.05), respectively. We conclude that absorption of sodium and chloride in the absence of organic solutes is mediated through different transport mechanisms at the luminal plasma membrane, which are in part subject to regulation by sodium and chloride transport inhibitors.
Biological Transport, Active↗
Effect of the somatostatin analogue SMS 201-995 on ATP-dependent calcium transport of basolateral vesicles from human duodenum.
Brush border membrane vesicles (BBMV) and basolateral membrane vesicles (BLMV) were simultaneously prepared from surgically resected pieces of morphologically intact human duodenum with a modified Percoll gradient centrifugation method. Alkaline phosphatase was enriched 20-fold in BBMV, whereas (Na+ + K+)-stimulated adenosine triphosphatase was enriched 15-fold in BLMV. BBMV and BLMV were preincubated with 3 microM synthetic somatostatin-14 or 3 microM SMS 201-995 for 10 min at 5 degrees C. In BBMV calcium, sodium, D-glucose, L-alanine, and D-mannitol uptake was unaffected by somatostatin-14 and SMS 201-995. In BLMV we found two Ca++ transport systems: Na+/Ca++ exchange and ATP-driven Ca++ transport. Somatostatin-14 had no effect on either of the two transport mechanisms. SMS 201-995 had no effect on Na+/Ca++ exchange but significantly inhibited basolateral ATP-dependent Ca++ transport (-40% +/- 5%, p less than 0.005).
Adenosine Triphosphate↗
[Deficiencies of the müllerian ducts induced by norethindrone in the female chick embryo].
Norethindrone produces two effects on müllerian ducts (MD) of female chick embryos. It induces the loss of the lower end of both ducts, as a result of a stop in their development, before 8 days. After 12 days NET causes regressions of the upper part of the MD particularly of the oviduct. NET like estrogens are the only known substances which present these both properties.
Abnormalities, Drug-Induced↗
[Reversible functional disorders of the intestinal tract caused by abdominal radiotherapy].
In 20 patients with malignancies receiving abdominal radiotherapy, tests for disturbed intestinal functions were performed at the beginning and at the end of the therapy and after 6 to 12 month following radiation. Four noninvasive tests were performed: 75Se-homotaurocholate (75SeHCAT) test for estimation of bile acid malabsorption; Schillings test for quantification of vitamin B12 absorption; H2-breath analysis before and after a test meal containing lactose as a parameter of lactose malabsorption; and 51Cr-EDTA test for estimation of intestinal permeability. Both bile acid and vitamin B12 absorption decreased significantly towards the end of abdominal radiotherapy in more than 50% of patients. Only one patient developed lactose malabsorption. After 6 to 12 month, these abnormalities had completely disappeared. In contrast, small intestinal permeability did not increase during radiotherapy but was significantly elevated 6 to 12 month following treatment as the only indication of chronic injury of small intestinal mucosa. During radiotherapy, a significant correlation existed between the severity of diarrhea and the degree of bile acid malabsorption.
Diarrhea↗
Functional defect of zinc transport in patients with Crohn's disease.
In the present study, we investigated the question whether the zinc deficiency in Crohn's disease is, at least partly, related to defective zinc entry at the jejunal brush border membrane. Brush border membrane vesicles (BBMV) were prepared from surgically resected pieces of morphologically intact human jejunum by a Mg++ precipitation method. We studied zinc, D-glucose, sodium and D-mannitol uptake into BBMV in 27 patients: 1) 20 patients with Crohn's disease 2) 7 controls. In 8/20 patients with Crohn's disease we found a significant decrease in zinc uptake (-25% +/- 5%, p less than 0.05) into BBMV. This impairment of zinc uptake in 40% of our patients with Crohn's disease was not associated with changes in the transport rates for D-glucose, sodium or D-mannitol. In addition no differences in enzyme marker concentrations or electron microscopic appearance were found.
Biological Transport↗
[Effects of testosterone and progesterone on the regression of Mullerian ducts induced by estradiol in the female chick embryo].
An administration of testosterone or of progesterone to estradiol-treated female chick embryos increased the rate of those presenting a regression of their Müllerian ducts. This observation favoured the hypothesis according to which the regression induced by estrogens depends on the anti-Müllerian hormone of ovarian origin.
Animals↗
[Physical performance of students participating in different sports].
Explore the source record for details and available documents.
Child↗
[Hypothalamo-hypophyseal correlates in the chick embryo: thyrotropic activity of adenohypophyseal grafts from donor embryos of various ages. Effect or pretreatment with TRH].
Adenohypophyses from 10 to 18-day-old chick embryonic donors grafted in 3 days chick embryos, thus disconnected from the hypothalamus, had a partially autonomous thyrotrophic activity. However this functional autonomy was greater in grafts from donors aged 10 or 11 days than from older embryos or from 11-day donors pretreated with T.R.H. before grafting. This strongly suggests that hypothalamo-adenohypophysis-thyroidal relationship establish normally between 11 and 12 days of embryonic life.
Animals↗
[Development of an embryonic thyroid grafted into a chick embryo].
A morphological and physiological study of an embryonic thyroid grafted in a chick embryo showed that it developed according to the endocrine status of the host. Its relative age appreciated at various stages of embryonic life is different from that of a gland developing normally during similar lengths of time.
Animals↗
[Synergistic action, on the masculinization of female chick embryo gonads, of a graft of embryonic testis and administration of an anti-estrogenic substance (tamoxifen)].
The capability of an embryonic testis graft to induce a male gonadal sex differentiation in genetically female chick embryos was significantly enhanced by the administration of a antiestrogenic drug (tamoxifen). This observation reinforces the view that the experimentally-induced testis differentiation depends chiefly on a inhibition of estrogen influence.
Animals↗