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Biomedical subjects

R Stein

Publications and source records attributed to R Stein.

At least 271 records · Page 15Linked to original sources
PubMed1983

Gold-195m for serial first pass radionuclide angiocardiography during upright exercise in patients with coronary artery disease.

Sequential first pass radionuclide angiocardiography can be performed in rapid succession using gold-195m because of its low radiation dose and short half-life (30.5 seconds). In 25 patients with known or suspected coronary artery disease, first pass studies with gold-195m were obtained using a computerized multicrystal gamma camera at rest (n = 29), at the end of each 3 minute stage of exercise (n = 25) and immediately after exercise (n = 23). In 13 patients, assessment of left ventricular function during exercise with gold-195m was combined with thallium-201 stress scintigraphy. Left ventricular ejection fraction at rest assessed with technetium-99m and gold-195m correlated well (r = 0.93). In addition, repeat left ventricular ejection fractions at rest with gold-195m correlated closely (r = 0.96). Comparing peak exercise left ventricular ejection fraction with ejection fraction at rest, abnormal left ventricular reserve was found in 20 of 25 patients. Various abnormal patterns of left ventricular ejection fraction response were noted, showing the diagnostic potential of serial exercise angiocardiography. Thallium-201 myocardial images, obtained on a single crystal gamma camera after multiple gold-195m injections, were all of good diagnostic quality and were abnormal in 10 of 13 patients. Thus, multiple high count rate first pass studies can be obtained with gold-195m during and after exercise, allowing serial study of physiologic changes in left ventricular function during exercise. Thallium-201 myocardial imaging can be performed using the same exercise test, providing direct comparison of myocardial function and perfusion.

Adult↗
PubMed1983

Pattern of methylation of two genes coding for housekeeping functions.

The distribution of sites that can be methylated was analyzed in the Chinese hamster adenine phosphoribosyl-transferase (aprt) gene and the patterns of methylation of this gene and the mouse dihydrofolate reductase (dhfr) gene were studied by using CpG restriction enzymes. Both genes were found to be unmethylated completely at their 5'-end region and methylated heavily throughout the rest of the gene. Because the hamster aprt gene can be inhibited by DNA methylation in vivo, the results suggest that 5' undermethylation of this gene may be a necessary condition for its expression. The pattern of methylation of each of these two genes was similar in sperm and all other somatic tissue DNAs. This is in contrast to many tissue-specific genes that were found to be highly methylated in sperm DNA and undermethylated in the tissue in which they are expressed. This result is consistent with the fact that both aprt and dhfr are key enzymes in the biosynthesis of nucleotides and therefore expected to be synthesized in all cells.

Adenine Phosphoribosyltransferase↗
PubMed1983

Isotopic and enzymatic IgE assays in non-allergic subjects.

In order to establish normal values in an adult population for serum IgE concentration, sera were obtained from 446 ambulatory Canadian caucasian subjects with negative allergy histories. A standard isotopic procedure, the Phadebas paper radioimmunosorbent test (PRIST), was compared with the new enzymatic counterpart, the Phadezyme PRIST. By the isotopic method, serum IgE concentrations in women and men were comparable from one age group to another with no age-related trend in the seven age groups examined (15-20, 21-30, 31-40, 41-50, 51-60, 61-70, above 70). The median and 95th percentile units (U)/ml respectively were 17.5 and 145 for 224 women and 25.5 and 275 for 222 men. Mean values +/- 1 SD for women were 43 +/- 102 and for men, 58 +/- 137. Levels were significantly higher in men as a group. Sera with IgE concentrations above 100 U and a sampling of additional sera were tested for specific IgE antibodies to 13 common allergens by the radioallergosorbent test (RAST). After exclusion of RAST-positive sera, the mean U/ml values +/- 1 SD were 22 +/- 29 for 204 women and 37 +/- 54 for 196 men. Geometric mean U/ml values for these sera were 14.6 for women and 22.3 for men and the median and 95th percentile U/ml respectively for the women were 15 and 66, for the men, 24 and 135. These 95th percentile values are considered the upper limits of normal in this population. The RAST identification of antibodies to allergens to which sensitization was demonstrated provided a potential explanation for serum IgE concentrations above 100 U/ml in less than 30% of the sera in this population with negative allergy histories. The isotopic method and the counterpart enzymatic method (Phadezyme PRIST) were highly comparable; the correlation coefficient (r) for all the sera was +0.93 (P less than 0.001). IgE levels were significantly higher in male smokers than non-smokers by both methods but these differences were not significant in women.

Female↗
PubMed1983

A home care program for children with chronic illness.

Pediatric Home Care (PHC) of Albert Einstein College of Medicine was designed as a special ambulatory care unit for children who are seriously or chronically ill and whose needs were not being met successfully through conventional programming. Services include: monitoring the patient's care: delivering direct services in the home, clinic, and hospital; teaching therapeutic programs to the family and patient; coordinating services; patient advocacy; health education; and supportive counseling. Each patient has a core team consisting of a generalist pediatrician, a PNP, and the patient's family. Visits are provided in the patient's home as needed, as well as in the traditional locations of the clinic, the inpatient units, and the PHC office. The program accepts patients with serious medical problems and who face any of a series of other difficulties, including: especially complex management problems that cannot be handled well in the customary outpatient settings; unstable family settings; or extended hospitalizations. Because it is not restricted to any single type of disease, the PHC provides a model that is applicable in a wide range of medical and community settings. Results of a program evaluation show that PHC is an effective intervention that has positive mental health outcomes and improves satisfaction with care.

Child↗
PubMed1983

Cellular location of N-acetyltransfer activities toward glucosamine and glucosamine-6-phosphate in cultured human skin fibroblasts.

The intracellular location in normal human cultured skin fibroblasts of the N-acetyltransferase activities that transfer the acetyl group from acetyl-CoA to the 2-amino group of glucosamine and glucosamine-6-phosphate have been investigated. Organelles have been separated using a combination of differential centrifugation and free flow electrophoresis. The intracellular distribution of the enzyme involved in the N-acetyltransfer to glucosamine and an alpha-glucosaminide disaccharide indicated that this enzyme activity concentrates mainly with lysosomal organelles whereas the activity associated with N-acetyltransferase to glucosamine-6-phosphate is non-lysosomal. It is proposed that acetyl-CoA: alpha-glucosaminide N-acetyltransferase may be used as a convenient enzyme marker of lysosomal organelle membranes.

Acetyltransferases↗
PubMed1982

Clonal inheritance of the pattern of DNA methylation in mouse cells.

DNA-mediated gene transfer was used to investigate the mode of inheritance of 5-methylcytosine in mouse L cells. Unmethylated phi X174 replicative form DNA remains unmethylated after its introduction and integration into these cells. On the other hand, phi X174 replicative form DNA that was methylated in vitro at its C-C-G-G residues retains these methylations as shown by restriction enzyme analysis with Hpa II and Msp I to detect methylation at this specific site. Although these unselected methylated vectors are prone to lose 30-40% of their methyl moieties upon transfection, this demethylation appears to be random. Once established, the resulting methylation pattern is stable for at least 100 cell generations. In order to examine the specificity of methylation inheritance, fully hemimethylated duplex phi X174 DNA was synthesized in vitro from primed single-strand phi X174 DNA by using 5-methyl deoxycytidine 5'-triphosphate. This molecule was inserted into mouse L cells by cotransformation and subsequently was analyzed by a series of restriction enzymes. Only methylations located at C-G residues were conserved after many generations of cell growth. The results suggest that the inheritance of the cellular DNA methylation pattern is based on a C-G-specific methylase that operates on newly replicated hemimethylated DNA.

5-Methylcytosine↗
PubMed1982

In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.

The effect of DNA methylation on the expression of the hamster adenine phosphoribosyltransferase (aprt) gene in mouse cells has been examined. This gene was methylated in vitro at all of its C-C-G-G sites by using Hpa II methylase and was inserted into mouse Ltk- aprt- L cells by cotransformation, with the herpes virus thymidine kinase gene as a selectable vector. Whereas clones carrying unmethylated aprt sequences were found to have an aprt+ phenotype as shown by their ability to grow in azaserine-containing medium, almost all clones carrying methylated aprt sequences were shown to be phenotypically aprt-. Blot hybridization analysis demonstrated that both the methylated and unmethylated aprt sequences were integrated into the cellular genome to the same extent and that the in vitro modification was stably maintained in these cells for many generations. When clones containing methylated aprt genes were exposed to conditions that select for the expression of the aprt gene, a low frequency of reversion to the aprt+ phenotype was observed. In all of these clones, this reversion was accompanied by reorganization and undermethylation of the aprt sequences. These results show that the expression of certain genes may be inhibited by site-specific methylation of these sequences and suggest that methylation may play a direct role in the regulation of gene expression.

5-Methylcytosine↗
PubMed1982

Effect of subconjunctival injection of hyaluronidase on outflow resistance in normal and in open-angle glaucomatous patients.

The effect of subconjunctivally injected hyaluronidase on ocular hydrodynamics was tested in 30 living human normal and 30 open-angle glaucoma eyes. It was found that after 1,500 TU of hyaluronidase injected subconjunctivally, the outflow resistance was significantly lowered: 34% in normal eyes, on the average; and by about 57% in the glaucomatous eyes. The possibility of either a higher amount of hyaluronic acid in the glaucomatous eyes or a normal amount of hyaluronic acid in the presence of compromised trabeculum is discussed. Clinical applications and further research are advocated.

Adolescent↗
PubMed1981

Properties of N-acetyl-beta-D-hexosaminidase from isolated normal and I-cell lysosomes.

Using a combination of differential centrifugation and free flow electrophoresis (Harms, E., Kern, H., and Schneider, J. A. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 6139-6143) a single population of highly purified lysosomes was obtained from normal, I-cell disease type 1, and I-cell disease type 2 cultured fibroblasts. Our findings indicate that most of the residual acid hydrolase activities remaining within the I-cell fibroblasts are localized in the lysosomes, analogous to normal cells. Characterization of the carbohydrate-dependent properties of the lysosomal N-acetyl-beta-D-hexosaminidase revealed that the I-cell and normal enzymes do not contain a significant proportion of neuraminidase-susceptible sialic acid residues, interact poorly with the beta-galactose-specific lectin Ricinus communis and are highly sensitive to endohexosaminidase H treatment, indicating that the oligosaccharide units of both the I-cell and normal lysosomal N-acetyl-beta-D-hexosaminidase are predominantly of the high mannose type. The I-cell and normal lysosomal N-acetyl-beta-D-hexosaminidase, however, differed in their endocytotic properties. In contrast to the high rate of endocytosis of the normal lysosomal enzyme (7.8%/mg/h), the I-cell type 1 lysosomal enzyme failed to be endocytosed into Sandhoff cells indicating an absent or altered phosphohexyl recognition marker on the I-cell enzyme. Examination of the normal extracellular N-acetyl-beta-D-hexosaminidase revealed the presence of predominantly high mannose-type oligosaccharide units, similar to the corresponding lysosomal enzyme, although properties typical of complex-type oligosaccharide chains were also evident. In contrast, the secreted I-cell enzyme revealed the presence of oligosaccharide units predominantly of the complex type indicating that the I-cell N-acetyl-beta-D-hexosaminidase has had high mannose-type oligosaccharide chains modified to complex-type probably in the Golgi or GERL region prior to secretion from the cell.

Acetylglucosaminidase↗