Biomedical subjects

R Stanislavov

Publications and source records attributed to R Stanislavov.

At least 19 recordsLinked to original sources

PubMed1991

[Folliculometry as a method for determining ovulation in insemination with frozen sperm].

The authors carried out studies retrospectively on 57 women, who became pregnant in insemination with donor frozen spermatozoa. Ovulation problems and luteal insufficiency were diagnosed in advance and treated before and during the occurring cycles. Women were divided into two groups. The first group of 25 women were examined by ultrasound transvaginal transducer for follow-up of follicle growth in the ovaries in addition to measurement of body temperature and determination of the cervical factor. The second group of 32 women, in whom follicle-metry was not performed. The frequency of conception was 0.24 in the first group, but 0.26 in the second group. It is concluded that inspite of the significance of follicle-metry for determination of ovulation the traditional methods are still valid criteria for ovulation and can be used as independent methods for determination of the time for insemination with therapeutic donor frozen spermatozoa.

Female↗

PubMed1990

[The effect of lectins on human spermatozoa in the capillary sperm penetration test].

The effect of the commercial lectin (Con A, PHA, SRA, WGA and UEA) as well as of the prepared by us extract from roots of Arum maculatum (Am and AM 80 degrees C) was studied on migration of human spermatozoa in bovine estrous cervical mucus, using the capillary sperm penetration test of Kremmer. It was established that Con A, PHA and AM inhibited the migration of spermatozoa in a concentration, in which they agglutinated human spermatozoa in vitro. Nonagglutinating human spermatozoa SBA and AM 80 degrees C inhibited also the migration of spermatozoa in the mucus.

Adult↗

PubMed1990

[Immunological studies of patients with chronic nonspecific prostatitis and infertility].

The authors studied carried out immunological studies on 20 men with chronic nonspecific prostatitis at reproductive age as well as on 10 healthy men included into the control group. Correlative investigations were made on the morphology of the ejaculate as well as on immunological parameters of seminal plasma aiming to establish the degree of the disturbed fertility. The immunological investigations included determination of albumin, transferrin and immunoglobulins in the seminal plasma. In men with chronic prostatitis there was a reduction in the number of spermatozoa up to 39 mln/cm3 combined with lower motility and an increased percentage of teratoforms. The immunological studies showed statistically significant nonincreased values in all men with chronic prostatitis in comparison with healthy men of the control group. These results show that increased permeability of barriers of blood serum proteins participates in the pathogenesis of the disturbed fertility. It is apparent that the cause for sterility in the married couples is due to autoimmune process occurring in men with chronic nonspecific prostatitis.

Adult↗

PubMed1989

[A method of determining dehydrogenase activity of human spermatozoa].

The authors present a quantitative method for the evaluation of biologic activity of human spermatozoa by determining their dehydrogenase activity by tetrazolium salts. The method is based on the property of these salts to change during biochemical reduction from colorless water soluble compounds into insoluble compounds in water compounds (phormazanes), which remain at the site of the reduction, e.g. in the cell. Most of the phormazanes are extracted by organic solvents (ethanol) and determined quantitatively by a spectrophotometer. We have measured dehydrogenase activity in spermatozoa by the amount of phormazane, obtained from a number of cells for a fixed time. We propose for determination of dehydrogenase activity in human spermatozoa: triphenyltetrazolium chloride, temperature of incubation at 37 degrees C, stopped by enzymic reaction by formaline, and creation of anaerobic conditions for incubation with Na2SO3.

Chemical Phenomena↗

PubMed1988