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Biomedical subjects

R Siebert

Publications and source records attributed to R Siebert.

122 records · Page 7Linked to original sources
PubMed1977

[Electroencephalographic examinations in emotionally disturbed children and adolescents (author's transl)].

Electroencephalographic examinations were conducted on 250 emotionally disturbed children and adolescents (175 boys and 75 girls) between the ages of 6 and 18. ll8 (47.2%) of the children and adolescents had an EEG which corresponded with their age group. 88 (35.2%) of the patients showed a HV alteration without any pathological graphologic elements. The electroencephalography in 44 cases (17.6%) clearly showed abnormal and/or pathological alterations. Frequently conspicuous findings were present in children with aggressive behavior, learning problems, anxiety syndromes as well as enuresis and encopresis. The alterations were less pronounced in those children who stuttered and the obsessive neurotics. No statistical significance, however, could be established. A correlation could not be established between a particular psychological symptom and a specific EEG alteration. Our results correspond in this regard with those presented in the literature. The majority of the authors, however, recorded conspicuous EEG findings much more frequently than we did. Basically, an electroencephalographic examination for emotionally disturbed children seems to be necessary for the recognition of somatic factors. For the evaluation, however, the range of variation of the normal encephalogram in children and its special characteristics at various age levels should be taken into consideration.

Adolescent↗
PubMedDate not supplied

Fluorescence in situ hybridization as a diagnostic tool in malignant lymphomas.

Primary and secondary chromosomal abnormalities play an important role in the characterization of biological, pathological, and clinical subgroups of malignant lymphomas. The introduction of fluorescence in situ hybridization (FISH) and the combination of immunophenotyping plus FISH to the diagnosis of lymphatic neoplasms allows the fast and sensitive detection of specific chromosomal changes and provides new insights into the genetic basis of lymphomagenesis. This article reviews the possibilities and limitations of molecular cytogenetic techniques in comparison to cytogenetic and molecular genetic methods and discusses their clinicopathological impact for non-Hodgkin's lymphoma and Hodgkin's disease.

Chromosome Aberrations↗
PubMedDate not supplied

[Conventional and multicolor FICTION as tools for the interdisciplinar study of hematological neoplasms].

Interdisciplinary technologies allowing a comprehensive characterization of cancer cells are becoming increasingly important in the routine diagnostics and research of hematological neoplasms, and in malignancies in general. One of those methods is the FICTION technique (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms). This technique allows the simultaneous study of the basic morphology as well as immunophenotypic and genetic markers of tumor cells. However, the low number of fluorescent substances utilized in a single assay restricts the number of markers that can be studied by FICTION. Recently, a multicolor variant has been developed and termed M-FICTION which in addition to cellular morphology and immunophenotype allows the investigation of multiple chromosomal aberrations in only one assay. In the present review, we present a detailed overview on the conventional and multicolor FICTION techniques and we discuss their multiple applications in the field of hematological neoplasms.

English Abstract↗
PubMedDate not supplied

[FICTION as a new tool to early lung cancer diagnosis].

Lung cancer is one of the most frequent causes of cancer death in Western countries. Overall 5-year survival rate is lower than 15% mainly due to the late diagnosis of the disease. Primary prevention (reduction of tobacco consumption) and more effective methods for early detection are needed. Some studies have recently shown that low-dose spiral computed tomography (CT) is a useful technique to the detection of pulmonary malignant nodules in early stages. Studies are developing to evaluate its efficacy in series of high-risk patients. A new cytogenetic technique has been developed: the FICTION technique (Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Neoplasms). This technique allows the simultaneous study of immunophenotypic markers and genetic abnormalities present in tumour cells. The goal of our project is optimise this technique in sputum and bronchoalveolar lavage specimens from lung cancer patients. The overall goal of this project is evaluate the usefulness of this technique, together with the new radiological techniques, in early detection programs of lung cancer in high-risk patients. In the present study we review the cytogenetic studies on lung cancer carried out in the recent years. We also introduce the basic methodological aspects that will be developed in our project.

English Abstract↗