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Biomedical subjects

R Seifert

Publications and source records attributed to R Seifert.

At least 163 records · Page 9Linked to original sources
PubMed1982

[Lithium therapy].

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Affective Disorders, Psychotic↗
PubMed1982

Comparison of direct and indirect two-site binding enzyme immunoassay.

Rabbit IgG, directed against HBsAg, was purified by positive and by negative affinity chromatography and applied in horseradish peroxidase labelled as well as in unlabelled form in the direct and indirect two-site binding enzyme immunoassay (EIA). Comparing direct and indirect assay the latter is more sensitive and less conjugate consuming. In contrast to the indirect assay in which antibodies, purified by positive affinity chromatography, do not alter detection limit, a 4- to 8-fold higher sensitivity was achieved in the direct EIA in contrast to antibodies, purified by negative affinity chromatography. In the indirect EIA unlabelled second and labelled third antibodies were incubated successively as well as simultaneously. The latter procedure shortened the assay time but needed antibodies purified by positive affinity chromatography and a 10-fold higher conjugate concentration. Greatest sensitivity was obtained in the indirect EIA by the use of labelled second and labelled third antibodies (20-30 ng/l HBsAg).

Chromatography, Affinity↗
PubMed1982

[Hypercaloric nutrition of patients with cancers of the head and neck region].

Patients with head and neck cancer and nutritional problems were treated by hypercaloric nutrition as supportive measure alongside surgery, radiotherapy, and chemotherapy. Improved healing of wounds after surgery and better tolerance of radiotherapy and chemotherapy proved the success of this measure. Enteral nutrition via naso-gastric tube was preferred to parenteral nutrition offering the possibility to continue nutrition on an outpatient basis. The amount of calorie intake depends primarily on patient compliance. Hypercaloric nutrition exercises a highly positive influence on basic cancer therapy of head and neck tumours.

Body Weight↗
PubMed1982

[Chemotherapy of head and neck squamous cell cancer with vindesine (Eldesine) and cis-platinum (Platinex) (author's transl)].

25 patients with advanced head-neck-squamous cell carcinoma were treated with a combination of Vindesine and Cis-Platinum. 12 patients were treated before surgery and/or radiation therapy. 25% achieved complete remission. 13 patients were treated for tumour recurrency after prior surgery and/or radiation therapy. 15% achieved C.R. Vindesine/Cis-Platinum is an alternativ scheme to Bleomycin combinations; even after prior RT and CT tumorreduction is frequent. Prior chemotherapy with following surgery and/or radiation therapy is recommended for the treatment of advanced head and neck tumours.

Adult↗
PubMed1981

Spontaneous mesangioproliferative glomerulonephritis in pigtailed macaques (Macaca nemestrina).

Mesangioproliferative glomerulonephritis was found in 28 of 113 pigtailed macaques (Macaca nemestrina) that died in 1977. In five it was considered severe enough to cause significant renal dysfunction; in two of these it was the cause of death. The basic lesion was a proliferation of mesangial cells and deposition of mesangial matrix in the mesangial stalk, resulting in various degrees of stalk expansion and increased lobulation of the glomerular tuft. Preliminary immunofluorescence and ultrastructural studies suggests the pathogenesis of the lesion may involve deposition of antigen-IgM immune complexes in the mesangial region.

Animals↗
PubMed1981

[Preoperative chemotherapy of squamous cell carcinoma of the head and neck with V-T-M schedule (author's transl)].

13 patients with far advanced squamous cell carcinoma of the head and neck were treated with a low dose combination chemotherapy of Vincristine, Bleomycin and Methrotrexate during 7--8 weeks prior to surgery or radio-therapy. V-B-M was given as an out- patient regimen. 9 patients were treated the first time ever, 4 patients suffered of tumor-recurrence. 5 patients i.e. 38% achieved complete remission of all measurable disease, a total of 9 patients i.e. 69% achieved a partial remission of more than 50%. Prior treatment or localisation of tumor did not influence the results. Competent application warranted, toxicity was mild. The significance of pre-operatively achieved complete remission is discussed. Short-term follow up does not yet permit any statement upon survival.

Adult↗
PubMed1981

[Palliative chemotherapy of inoperable squamous cell cancer of the head and neck with V-B-M-schedule (author's transl)].

12 patients with far advanced head and neck squamous cell carcinoma, no longer apt to curative therapy, were submitted to palliative chemotherapy by V-B-M- schedule. Palliative therapy has great importance in this type of tumor, which due to its localisation causes terrible deformities and thus psychologically and sociologically forms a heavy burden. Nearly all patients became free of pain, exulcerations could be reduced, successfully general status could be improved. Due to an out patient regimen patients remained at home. Most of all this last circumstance helped to realize true palliative therapy; toxicity was mild.

Bleomycin↗
PubMed1981

Effect of IgG-horseradish peroxidase conjugates purified on Con A-Sepharose upon sensitivity of enzyme immunoassay.

Antibodies coupled to horseradish peroxidase (HRP) were purified by gel filtration and by combination of affinity chromatography and gel filtration. Binding kinetics to solid phase bound antigens were studied of the antibody-enzyme conjugates in the presence and in the absence of unlabelled IgG. The detection limit in enzyme immunoassay was found to be lowered by factor 16 after elimination of unlabelled IgG from the conjugate.

Animals↗
PubMed1979

In vitro haemolysis from adrenochrome in the blood of schizophrenic patients, revised.

The recently reported abnormal in vitro haemolysis from catecholamine metabolites in schizophrenia was tested. We did not find the postulated increase in haemolysis, though the exchange of plasma for Ringer solution renders some patients' erythrocytes more susceptible to the effects of adrenochrome. The possibility of a subgroup of schizophrenia with a defect in membrane stability is discussed.

Adolescent↗
PubMed1975

[Rationalized lithium adjustment by load-test (author's transl)].

The lithium blood level 24 hrs after a priming dose of lithium is a prognosticator for the therapeutic dosage required. Exactly 24 hrs following the intake of 24 mval lithium (lithium carbonate, retard form) venous blood serum is analyzed for lithium content. The necessary therapeutic dose can be inferred from a regression line which shows the negative interrelation between the 24 hr value and the therapeutic quantity of lithium necessary to achieve a blood level of 0.9 mval/1 under steady state conditions. Low 24 hr levels require high maintenance doses and vice versa. This procedure shortens the time of dose adjustment and avoids undesirable side-effects in the beginning of treatment which otherwise are not seldom the cause for the cessation of lithium intake.

Adolescent↗
PubMed1975

[Diagnostic aspects of complex blood-protein analyses in screening].

During a screening at a testing of 5 different protein parameters (IgG, IgA, IgM, Hp, Tf) different types of distribution were found. While IgG, Hp and Tf have a normal distribution, IgG and IgM are not normally distributed in the uncleansed group. Measures of elimination of suggested sizes of influence ("liver parameters") remained without effect.

Blood Proteins↗