Biomedical subjects
Publications and source records attributed to R Sasaki.
Explore the source record for details and available documents.
Three hundred cases of maxillary sinus carcinoma were divided into four groups according to the initial treatment: the first group was treated by irradiation of 5,000 rad (25 fractions) for 5 weeks; the second by intra-arterial infusion of 5-FU combined with irradiation; the third by sinus curetting in accordance with Denker's operation combined with the second method; and the fourth by cryosurgery once a week during the second irradiation method followed by sinus curetting at the end of initial treatment. Histologically confirmed recurrence was removed by maxillectomy, if possible. The three year cumulative survival rates in the four groups were 27.1% (first group), 55.1% (second group), 53.5% (third group) and 53.1% (fourth group). The three year local non-recurrence rates after initial treatment without maxillectomy were 26.4%, 31.1%, 55.8% and 45.4%, respectively. The frequency of distant metastasis without local recurrence was as high as 12.3% in the third group and conversely 5.6% in the fourth. These results demonstrated the usefulness of cryosurgery.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Sialyltransferase activity in blasts from acute lymphoblastic leukemia (ALL) was markedly lower (1.68 +/- 1.23 pmol/mg protein) than those (6.18 +/- 2.22 pmol/mg protein) of lymphocytes from normal volunteers (t less than 0.001). On the contrary, enzyme activity was significantly increased in blasts (1.21 +/- 0.38 pmol/mg protein) from acute non-lymphoblastic leukemia, compared to the level (0.53 +/- 0.32 pmol/mg protein) of mature granulocytes (t less than 0.001). In TdT-negative CML in blast crisis, sialytransferase activity (2.11 +/- 0.88 pmol/mg protein) was significantly higher than those of mature granulocytes (t less than 0.001), whereas no significant difference in the enzyme activity was noted between the blasts from TdT-positive CML in blast crisis and from ALL. In TdT-positive ALL cases, there was an inverse relationship (r = -0.85, t less than 0.01) between sialytransferase activity and terminal deoxynucleotidyl transferase (TdT) activity of the blasts. Therefore, sialytransferase in leukocytes may be a unique enzyme in which changes in activity relate to the differentiation or malignant transformation of leukocytes.
A doublet signal was observed in human serum by the ESR technique at room temperature. This radical had a g value of 2.0054 and a hyperfine splitting constant of 1.84 gauss and was assigned to ascorbate radical. The ascorbate radical in serum was very stable. The intensity of ESR signal showed no differences between serum and plasma of the same individual. Photosensitivity of the ascorbate radical in serum and sodium ascorbate solution was examined and enhancement of ESR signal by irradiation was observed, although the responses in serum and in ascorbate solution were considerably different. The intensity of ESR signal was proportional to the concentration of ascorbate solution. The ESR intensity of ascorbate radical can be used as a reliable method for quantitative estimation of ascorbate radical.
Effect of dexamethasone of the growth of two T-cell acute lymphoblastic leukemia (T-ALL) cell lines, JM and TALL-1 and an American Burkitt lymphoma cell line, Ramos, of B-cell type was examined. While the growth of both TALL-1 and Ramos cell lines was significantly inhibited by dexamethasone at the concentration of 10(-7) M, JM cell line was markedly resistant at the high concentration of 10(-5) M of dexamethasone. The assay of glucocorticoid receptors showed that a small amount of the binding site was found in both T-cell leukemia cell lines, JM and TALL-1 and that a moderate amount of the binding site was found in Ramos cell line. Thus, glucocorticoid sensitivity (growth inhibition of these leukemia-lymphoma cell lines by dexamethasone) did not correlate with either the number of glucocorticoid receptor or with surface membrane markers of the target cells. This result suggests further marked heterogeneity of leukemic cells.
Explore the source record for details and available documents.
The level of sialyltransferase activity in leukaemic blasts from acute lymphoblastic leukaemia (ALL) cases was significantly lower (3.29 +/- 2.09 pmoles/5 X 10(7) cells or 1.77 +/- 1.16 pmoles/mg protein) than those (18.80 +/- 4.91 pmoles/5 X 10(7) cells or 7.72 +/- 1.75 pmoles/mg protein) of mature lymphocytes from normal volunteers (T less than 0.001). An inverse relationship between the level of sialyltransferase activity and the level of terminal transferase (TdT)activity was seen in blasts from eight TdT-positive ALL cases. No significant difference was observed in the level of sialyltransferase activity between ALL and cells of chronic myelogenous leukemia (CML) in blast crisis. Short Term culture of ALL blast cells with 12-0-tetradecanoylphorbol-13-acetate (TPA) at the concentration of 10-(6)M to 10-(9)M caused a marked increase in sialyltransferase activity. In one of these three ALL cases the population of TdT-positive cells and the TdT activity of the blasts decreased significantly after culture with TPA. These results suggest that biochemical differentiation of leukaemic lymphoblasts has been induced by the addition of TPA, although morphological changes were not observed. Sialyltransferase activity may be a useful indicator for the analysis of differentiation of lymphocytes.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
In human cancers, the contribution of genetic determinants is often difficult to distinguish from environmental influences. However, some cancers are inherited in Mendelian pattern, and some have closely associated with the diseases with hereditary factors. This paper reviewed characteristics of hereditary cancers, the diseases with high incidence of cancers and familial aggregation of cancers, and discussed the role of host factors in the etiology of cancer clinico-epidemiologically.