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R Sanchez

Publications and source records attributed to R Sanchez.

At least 145 records · Page 8Linked to original sources
PubMed1989

Empathy, diversity, and telepathy in mother-daughter dyads: an empirical investigation utilizing Rogers' conceptual framework.

The purpose of this study was to examine empathy, diversity, and telepathy in natural mother-daughter dyads. The investigation was conceptualized and conducted according to the concepts and principles of the Rogerian Science of Unitary Human Beings. The sample for this descriptive study consisted of 180 volunteer mother-daughter dyads; the mother served as the receiver and the child as sender of telepathic messages. Empathy and diversity were expected to be related to telepathy levels. Results indicated that one form of empathy (personal distress) and diversity were significantly (p less than .05) related to telepathy. When the components of the diversity score were further examined, only those subjects who were moderately differentiated and mobile demonstrated significant telepathy scores. Multiple regression analysis showed that 6% of the variance in telepathy was accounted for by diversity and empathy. Findings added to what is known about living systems that manifest negentrophy and evolve toward increasing diversity.

Adult↗
PubMed1988

Inhibition of stress-induced hyperglycemia by tail pinching or intraventricular enkephalin administration in the rat.

The tail pinch (t-p) method added to a basal restraint stress produced inhibition of the stress-induced hyperglycemia, an effect that was neutralized with intrathecal anesthesia but not with intracerebroventricular (i.c.v.) naloxone (50, 100, 1000 ng/100 g) or with intraperitoneal naloxone injections (0.1-0.3 mg/100 g). A similar negative result was obtained with i.c.v. administration of 500 and 1000 ng/100 g of beta-endorphin. In contrast, a single i.c.v. injection of 1000 ng/100 g of Met-enkephalin reproduced the t-p inhibitory effect. The latter was not elicited by i.c.v. FK 33824, an enkephalin analogue, a result that supports the specific participation of the delta-opioid receptors. The results obtained with central alpha-adrenoceptor antagonists and central noradrenergic chemical destruction, or central alpha-adrenoceptor agonists, support the production of a reinforcement of the alpha-adrenoceptor stress stimulation by the t-p procedure, probably through noradrenaline and enkephalin mediation.

Animals↗
PubMed1988

Oral lesions of lupus erythematosus patients in relation to other chronic inflammatory oral diseases: an immunologic study.

The nature and distribution of mononuclear cells in non-ulcerated oral lesions of discoid (DLE) and systemic lupus erythematosus (SLE), were investigated and compared to other chronic inflammatory oral diseases (lichen planus (LP), contact lesion (CL), unspecified inflammation (UI), geographic tongue (GT), and leukoplakia (LK). For this purpose an immunoperoxidase technique based on staining with monoclonal antibodies was employed. In most LE specimens examined infiltrating cells consisted predominantly of a mixture of T cells (Leu 3a+ and Leu 2a+) that were distributed in the lamina propria, the submucosa, and occasionally also in the epithelium. In general, only few B cells were detected while macrophages were more frequent. In all LE specimens examined beta 2-microglobulin expression was observed on a large proportion of cells including infiltrating mononuclear cells as well as resident keratinocytes. In addition, most infiltrating cells displayed MHC Class II antigens according to a pattern HLA-DR greater than DQ greater than DP. Interestingly, expression of Class II antigens was also observed on epithelial keratinocytes but was restricted to HLA-DR and -DP gene products (DR much greater than DP). HLA-DQ expression was never observed on keratinocytes. In most LE specimens studied a small proportion (less than 5%) of inflammatory cells had detectable interleukin-2 receptors (IL-2R) and/or transferrin receptors (transf-R). However, expression of transf-R was also observed on basal epithelial cells, being more pronounced in DLE than in SLE lesions. The above staining patterns observed in LE lesions, when compared to other chronic inflammatory oral lesions, did not disclose any striking differences that could support the specific diagnosis of LE. However, the findings of Class I and II MHC gene products on oral keratinocytes suggest an important accessory role for these cells in directing the migration of activated lymphoid cells in the epithelium in chronic inflammatory lesions of the oral mucosa.

Histocompatibility Antigens Class I↗
PubMed1988

Phenotypes of immunocompetent cells and Ia antigen expression in oral mucosa and skin of autoimmune mouse strains.

The phenotypic characteristics of resident and infiltrating cells in skin and oral mucosa of MRL/Mp-lpr/lpr (MRL-lpr), MRL/Mp+/+ (MRL-(+)), NZB x NZW F1 (NZB/W) and Balb/c mice have been studied using monoclonal antibodies (Mabs) and immunoperoxidase staining with the Avidin-Biotin Complex method. Macroscopically evident skin lesions appeared spontaneously exclusively in aging MRL-lpr mice. Infiltration of lymphocytes was detected within the mucosa and the skin of MRL-lpr-mice. These lymphocytes expressed predominantly L3T4 and to a lesser extent Lyt-2 phenotype (T helper/inducer and T suppressor/cytotoxic subset, respectively). Class II major histocompatibility complex antigen (Ia) expression was detected on macrophages, dendritic cells and few lymphoid cells in normal oral mucosa and skin of all strains examined. However, in the oral mucosa and skin lesions of the 4 months old MRL-lpr mouse also keratinocytes expressed Ia antigens. Keratinocytic Ia expression was also detected in oral mucosa of 8 months old MRL-(+) mice. The described immunopathological findings in skin and oral mucosa of old MRL-lpr mice show a number of important similarities to human SLE disease.

Aging↗
PubMed1988

[Benign vulvovaginal tumefactions in children. General review illustrated by a series of 57 personal cases].

There are numerous etiologies of benign vulvo-vaginal tumefactions in children. However, the etiological diagnosis can usually be based on clinical data after careful examination. Para-urethral cysts are the main cause of labial and vestibular tumefactions; they are generally cured by simple puncture. Solid vaginal tumors are uncommon, but they always require surgical resection in order to exclude the exceptional and severe malignant tumours. Neonatal hydrocolpos is frequent in our experience and must always be treated surgically; associated malformations are frequent and must be systematically searched for. Ectopic prolapsed ureterocele and circular prolapse of the urethral mucosa are easily recognizable and need surgical treatment.

Adolescent↗
PubMed1988

Features of renal vasculitis in autoimmune MRL lpr/lpr mice: phenotypes and functional properties of infiltrating cells.

MRL lpr/lpr (MRL/1) mice spontaneously develop a widespread renal vasculitis. The majority of the cells in vasculitic lesions are bright Ly-1, L3T4 and la-positive in contrast to the cells found in lymph nodes and spleens of the old MRL/1 mice. However, despite differences in phenotypical patterns, B and T cells from arteritic lesions do not differ from mononuclear cells (MNC) eluted from MRL/1 lymph nodes with regard to the frequency of IgG secreting cells and the proliferative responses to Concanavalin A (Con A). Co-culture experiments with congeneic MRL+/+ (MRL/n) spleen cells indicate that the poor response to Con A of the MNC eluted from vasculitic lesions is, unlike the case of lymph node MNC, due to suppressive action of vasculitic cells on the indicator cell population. Further support for the activation status of infiltrating MHC in kidney vasculitic lesions, expressed by high in vivo uptake of 3H-thymidine, was obtained by autoradiography performed on frozen sections. The observed differences in phenotypic patterns and functional features between lymph node MNC and infiltrating vasculitic MNC indicate that different immune mechanisms may be responsible for the development of lymphadenopathy and vasculopathy, respectively in MRL/1 mouse.

Animals↗
PubMed1987

Lysyl-tRNA synthetase from Escherichia coli K12. Chromatographic heterogeneity and the lysU-gene product.

In contrast with most aminoacyl-tRNA synthetases, the lysyl-tRNA synthetase of Escherichia coli is coded for by two genes, the normal lysS gene and the inducible lysU gene. During its purification from E. coli K12, lysyl-tRNA synthetase was monitored by its aminoacylation and adenosine(5')tetraphospho(5')adenosine (Ap4A) synthesis activities. Ap4A synthesis was measured by a new assay using DEAE-cellulose filters. The heterogeneity of lysyl-tRNA synthetase (LysRS) was revealed on hydroxyapatite; we focused on the first peak, LysRS1, because of its higher Ap4A/lysyl-tRNA activity ratio at that stage. Additional differences between LysRS1 and LysRS2 (major peak on hydroxyapatite) were collected. LysRS1 was eluted from phosphocellulose in the presence of the substrates, whereas LysRS2 was not. Phosphocellulose chromatography was used to show the increase of LysRS1 in cells submitted to heat shock. Also, the Mg2+ optimum in the Ap4A-synthesis reaction is much higher for LysRS1. LysRS1 showed a higher thermostability, which was specifically enhanced by Zn2+. These results in vivo and in vitro strongly suggest that LysRS1 is the heat-inducible lysU-gene product.

Adenine Nucleotides↗
PubMed1987

Polytetrafluoroethylene microprosthesis in the arterial system of the rat.

The experimental behavior of a 1-mm internal diameter (i.d.) polytetrafluoroethylene (PTFE) microprosthesis, as a substitute for an abdominal aortic segment in the rat, was reviewed. Fifty Wistar rats were divided into four groups: Group I--12 rats with autotransplant of an abdominal aortic segment (AAS); Group II--12 rats with allotransplant of an AAS obtained from Long-Evans rats; Group III--12 rats with xenotransplant of an AAS taken from rabbit femoral arteries; and Group IV--14 rats with substitution of an AAS by a 1-mm i.d. PTFE microprosthesis. The rats were sacrificed at different time intervals ranging from five to 360 days, with previous aortography. In Group I, there was a 100 percent patency at a mean of 152.41 days; in Group II, a 91.6 percent patency at a mean of 100.08 days; in Group III, an 83.3 percent patency with a 75 percent aneurysmal dilation at a mean of 107.58 days; in Group IV, a 71.42 percent patency with two anastomotic aneurysms at a mean of 105 days (P less than 0.05, chi square) between Groups I and IV, autotransplant vs. PTFE). The 1-mm PTFE microprosthesis placed in the arterial system of the rat proved to be a reliable alternative for microvascular substitution.

Animals↗
PubMed1987

Polytetrafluoroethylene microprosthesis in the venous system of the rat.

In this study, a 2 mm internal diameter (i.d.) polytetrafluoroethylene (PTFE) microprosthesis was used in the venous system of the rat, to determine whether or not it could serve as an acceptable microvenous substitute. Forty Long-Evans rats were divided into four groups: Group 1-10 rats with autotransplant of an inferior vena cava segment; Group 2-10 rats with a segment substitution of the inferior vena cava by a 2 mm i.d. PFTE microprosthesis; Group 3-10 rats with a laterolateral portacaval shunt; and Group 4-10 rats with a portacaval shunt and interposition of a 2 mm i.d. PTFE microprosthesis (new model). The rats were sacrificed at different time intervals up to 100 days, with cavography (femoral access) in Groups 1 and 2 and spleenoportography (direct puncture of the spleen) in Groups 3 and 4, before sacrifice. In Group 1, a 100 percent patency was observed at a mean of 49 days; in Group 2, a 70 percent patency with a 30 percent stenosis at a mean of 39.4 days (p less than 0.05); in Group 3, a 100 percent patency at a mean of 42.5 days; and in Group 4, a 30 percent patency at a mean of 38.4 days (p less than 0.01). Results showed that the 2 mm i.d. PTFE microprosthesis placed in the venous system of the rat is not an efficacious procedure, and that the search for better microvenous substitutes should focus on those of biologic origin.

Animals↗
PubMed1987

D-penicillamine and beta-aminopropionitrile effects on experimental filtering surgery.

We studied the effect of two compounds, beta-aminopropionitrile (BAPN) and D-penicillamine on the success of experimental glaucoma filtering surgery in cynomolgus monkeys. All animals had laser-induced glaucoma and underwent punch sclerectomy operations. Eyes treated with BAPN or D-penicillamine maintained successful filtration for at least 3 days longer than nondrug-treated controls. This effect, while statistically significant (P less than 0.05), was temporary. The lack of more substantial improvement in prolonging filtering patency may have resulted from an inadequate effective drug concentration. Alternatively, these drugs may have limited potency because their action on new collagen synthesis affects only a minor component of the healing process that causes filter failure.

Aminopropionitrile↗
PubMed1986

Permethrin 1% creme rinse for the treatment of Pediculus humanus var capitis infestation.

Permethrin 1% creme rinse (NIX) was tested as a treatment for Pediculus humanus var capitis (head lice) in a placebo-controlled, double-blinded, randomized study. As a positive control, a third arm of the study included nonrandomized, but investigator-blinded, treatment with 1% lindane shampoo (Kwell). At 14 days after treatment, 97% of patients treated with permethrin were free of lice compared to 6% of placebo-treated patients (P less than 0.001) and 43% of the lindane-treated group. Permethrin was 70% ovicidal compared to 14% for placebo (P less than 0.001) and 45% for lindane. No adverse experiences were noted during this study. Permethrin 1% demonstrated high pediculicidal and ovicidal activities, which in combination with its low mammalian toxicity, residual activity, and cosmetic properties, make it an excellent treatment for pediculosis capitis.

Adolescent↗
PubMed1985

Optic nerve head blood flow in chronic experimental glaucoma.

We used the tritiated iodoantipyrine method to estimate blood flow in the retina, optic nerve head, and retrobulbar optic nerve in monkey eyes. Twelve eyes with short-term intraocular pressure (IOP) elevation and nine eyes with long-term (up to 20 months) IOP elevation were compared with their fellow normal eyes. The eyes with long-term glaucoma were staged for degree of optic nerve damage according to disc cup size, nerve fiber layer atrophy, and histologic estimates of the remaining number of nerve fibers. At normal IOP levels, there were only minor differences in blood flow in the superior, inferior, and middle nerve head sections. In eyes with short-term IOP elevation, nerve head blood flow was normal, unless IOP exceeded 75 mm Hg. Above this level, a progressive decrease in nerve head blood flow was measured. Mean blood flow in the nerve head of eyes with long-term glaucoma was not significantly different from that in the normal fellow eyes. Some animals had small increases in blood flow, while others had small decreases. These differences from normal did not correlate with the amount or location of optic nerve damage. Our data do not support decreased blood flow as part of the pathogenesis of long-term experimental glaucoma.

Animals↗
PubMed1985

Crush injury of the carpus.

Severe crush injuries affecting the bones of the carpus are rare. We review the nine cases that have previously been described and report four additional cases which we have treated and followed up. All 13 present remarkable similarities, with disruption of the carpal arch through the capitate-hamate articulation distally and the piso-triquetral joint proximally. Three slightly different variations of this pattern of fracture-dislocation are identified. The flexor retinaculum must be involved to allow the displacement which is seen, and there is usually an extensive laceration of the thenar eminence. Treatment by closed reduction is usually successful. Long-term follow-up shows that, in the absence of nerve or vascular damage, the results are surprisingly good.

Adolescent↗