The effect of intense visible light on cellular respiration.
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Biomedical subjects
Publications and source records attributed to R S Olson.
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Freshly prepared hemoglobin solutions were successively irradiated up to five times with 1 MW (monochromatic wavelength) of green (530 mmicro) laser power. Oxygenated hemoglobin showed no detectable change, but the spectral absorption of reduced hemoglobin showed a shift toward the characteristic curve for the oxygenated form. Intact human erythrocytes exposed to a power density of 110 MW/cm(2) of green laser radiation showed no appreciable change in diameter or mass, but they became transparent to a wavelength range from 400 to 600 mmicro. A similar power density from a ruby laser failed to produce this bleaching effect. This response in the erythrocyte demonstrates a principle which suggests the laser as a tool for cell research: specific molecular components within a cell may be selectively altered by laser irradiation when an appropriate wavelength and a suitable power density are applied.
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Halo skeletal traction may be used to immobilize a patient's head and neck following a cervical fracture. Prevention of infectious reactions around the pin sites of halo traction is a priority in rehabilitation nursing practice. Although prophylactic treatment of skeletal pin sites is a generally accepted practice, there is great diversity of opinion and very limited systematic investigation as to how the sites should be treated. In this article, the author reviews the literature on pin site care and suggests a protocol based on the current literature.
This descriptive study was designed to explore the attitudes of rehabilitation nurses, occupational therapists, and physical therapists toward people with disabilities. Attitudes were measured by using the Attitudes Toward Disabled Persons Scale, Form B (ATDP-B). The sample included 150 rehabilitation nurses, 57 occupational therapists, and 43 physical therapists in southeast Texas. Findings suggest that the majority (67%) of the healthcare professionals had positive attitudes toward people with disabilities. Occupational therapists had significantly higher scores than the rehabilitation nurses and physical therapists. Practice setting, age, educational level, and amount of experience had no significant effect on attitude scores. ATDP-B scores tended to increase with work experience, but not to a statistically significant degree. An analysis of the rehabilitation nurses' attitude scores showed that practice setting, age, and educational level had no significant effect. Experience seemed to be correlated with higher attitude scores, although the relationship was not statistically significant.
Staying abreast of the numerous recent advances in diabetes management presents a challenge to healthcare professionals. This paper provides an overview of these changes and discusses their implications for rehabilitation caregivers and clients. Because of increased scientific knowledge of the disease, there have been changes in nomenclature, diagnostic criteria, and nutritional guidelines. Medications to improve control of diabetes and innovations in equipment have been developed. The concepts of intensive management and self-management have gained importance, as have reimbursement issues. An overview of these advances and their implications for nursing practice, may help nurses throughout the continuum of care to assist individuals with diabetes to enjoy the maximum quality of life possible.
Young [16-19 population doubling level (PDL)] and senescing (50-53 PDL) WI-38 cell populations were exposed to 1 ppm ozone for 2 hr and the resultant extracellular and intracellular acid phosphatase concentration was measured. Dose-response curves were also determined for surviving populations of young and old cells after a 1 hr ozone exposure ranging in concentration from 0 to 1.00 ppm. Senescing cells released 8 times more acid phosphatase per million cells than the young cells. Both old and young cells showed a clear dose-response to the 1 hr ozone gradient exposure. However, the older cells demonstrated a consistent 17% average lower survival rate than the young cells. The higher acid hydrolase level in older WI-38 cells is probably related to the lower survival rate observed in the older cells in vitro.
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