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Biomedical subjects

R Roth

Publications and source records attributed to R Roth.

At least 37 records · Page 2Linked to original sources
PubMed1988

A prospective study of the incidence of urinary incontinence in an acute care teaching hospital and its implications on future service development.

OBJECTIVE: To assess the incidence of urinary incontinence and indwelling catheter use. METHOD: A prospective study of 627 consecutive admissions to an acute care teaching hospital over a 4-week period. RESULTS: At least one episode of urinary incontinence was experienced by 145 patients (23.4 per cent of all admissions) with 28 per cent of these having urinary incontinence lasting more than 10 days. Patients with urinary incontinence had a significantly greater age, mean length of stay and overall mortality compared with the total hospital inpatient population. During this survey, 7 per cent of all inpatients suffered from at least one episode of faecal incontinence, with the mean duration of faecal incontinence being 5.4 days. The major discriminating findings were from the data of patients with urinary incontinence for longer than 10 days. These patients had a longer length of stay (47 days compared with 17 days for patients with urinary incontinence 10 days) and poorer outcome (28 per cent went to nursing homes and 50% returned home compared with 7 per cent and 81 per cent respectively for pts with incontinence less than 10 days). Patients on medical wards and those with confusion or poor mobility fared disproportionately worse. After 10 days of incontinence, only 59 per cent of these patients had a rectal examination; and 90 per cent a urine specimen for culture. The interpretation and implication of these findings will be discussed particularly as it related to future planning for continence services and the development of clinical nurses with knowledge of urinary incontinence.

Adult↗
PubMed1987

Use of a monoclonal antibody (VM-2) plus the immunogold-silver technique to stain basal cell carcinoma cells.

A newly described murine monoclonal antibody, VM-2, stains both basal cell carcinoma cells and squamous cell carcinoma cells of the skin. We have combined this monoclonal antibody with a newly described technique, the immunogold-silver stain, to produce a method for rapid characterization of basal cell carcinoma cells. This method does not require the use of a fluorescence microscope and gives a very intense stain of the tumor cells. Tumor-containing tissue from 12 patients was stained and included 2 cases of morphealike basal cell carcinoma. All the tumors stained with VM-2 gold. Thus, staining with this technique may be helpful in diagnosing tumors of epithelial origin and may also find application in delineating residual tumor cells in microscopically controlled excision.

Antibodies, Monoclonal↗
PubMed1987

Intussusception: a supplement to the mnemonic for coma.

Expected clinical manifestations of intussusception include paroxysmal abdominal pain, vomiting, abdominal mass, and with time, rectal bleeding. We report a case where lethargy and vomiting are the presenting complaints. Diagnostic delay was encountered for this infant who had altered sensorium without accompanying pain, melena, or mass on initial examination. Either plain radiographs, supplemented by ultrasonography of the abdomen, or a barium enema should be performed in infants with unexplained lethargy.

Coma↗
PubMed1986

[Behavioral medicine aspects of juvenile diabetes].

Childhood or insulin-dependent diabetes is a life-long illness, placing complex and daily demands on the patient and the family. Some studies were carried out in order to investigate the relationship between health status, behavioral and psychological variables. The Hemoglobin A1 was used to index glycemic control. A newly developed diabetes knowledge test and a questionnaire on diabetic related beliefs were administered. The results indicate that diabetic control improves with age. General knowledge of diabetes increases with age, but relates not to diabetic control. But considering age and the particular subtests of knowledge as predictors 59.44% of the variance in diabetic control can be estimated. Perceived barriers to implementing self-care and feelings of disruptiveness culminate in the age of 12 to 13 years. Feelings of stigmatization and the affection by the disease loose weight as disturbing factors with increasing age and knowledge. Increased attention to the child's cognitive developmental level is suggested and methods for improving the effectiveness of patient intervention programs are discussed.

Adolescent↗
PubMed1986

[Neurocysticercosis. Diagnostic and therapeutic advances].

A 42-year-old man of Croatian birth, with long-standing neurological symptoms, was found to have neurocysticercosis (a rare disease in Central Europe), as proven by positive antibody titres in serum and CSF and typical foci by computed tomography (CT). Ventriculo-cisternal drainage (after Torkildsen) controlled the acute symptoms of raised intracranial pressure. Postoperatively the CT revealed new low-density intracerebral foci, which responded to praziquantel.

Adult↗
PubMed1985

Thiopyronine and 8-methoxypsoralen sensitized photodynamic effect on DNA synthesis in yeast.

The synthesis of DNA in growing yeast cells was investigated after photodynamic treatment of the cells with thiopyronine (TP) and visible light or with 8-methoxypsoralen (8-MOP) and UVA light. DNA synthesis was inhibited after photodynamic treatment with 8-MOP but not after photodynamic treatment with TP. This result is further evidence that the photodynamic effect with TP does not attack nuclear DNA in eucaryotic cells.

DNA, Fungal↗
PubMed1985

Therapy of symptomatic pyelonephritis in women.

A prospective randomized controlled study was performed on 44 women with community-acquired, uncomplicated pyelonephritis requiring hospitalization. The study was designed to determine if an extended course of therapy (21 days) would be more effective than the conventional 10-day course of therapy. All patients initially received either gentamicin or tobramycin parenterally for 48 to 72 hours, and then were prescribed an oral agent that possessed in vitro inhibitory activity for the urinary pathogen to complete the course of therapy. Duration of medication was assigned according to a table of random digits. Of the 22 women receiving the 10-day treatment 17 were cured, 4 experienced a reinfection and none had a relapse, compared to 12, 5 and 3, respectively, of 22 receiving the 21-day treatment. Analysis of the therapeutic failures did not demonstrate any superior response for either treatment program (p less than 0.75 and greater than 0.50). No statistically increased incidence of adverse drug reactions occurred in patients receiving the extended course of therapy. Our data do not support an extended course of therapy in women with acute symptomatic pyelonephritis uncomplicated by structural abnormalities.

Adolescent↗
PubMed1983

Use of a murine monoclonal antibody which binds to malignant keratinocytes to detect tumor cells in microscopically controlled surgery.

A new murine monoclonal antibody, termed VM-1, stains basal cell carcinoma cells and at least some squamous cell carcinoma cells. This antibody was used to visualize the tumor margins during microscopically controlled surgery using immunofluorescence (IF) and immunoperoxidase (IP) technics. Our experiments demonstrate that VM-1 is helpful in detecting small islands of tumor cells in the tissue at the margins of skin cancers. Thus, tissue staining with this antibody should improve the accuracy of microscopically controlled excision, and hopefully will decrease the rate of tumor recurrence after surgery.

Animals↗
PubMed1979

A rapid, inexpensive and easily quantified assay for phagocytosis and microbicidal activity of macrophages and neutrophils.

The objective of the present study was to develop a technique to quantitate the phagocytic and intracellular microbicidal activity in different populations of phagocytes, i.e. neutrophils and macrophages. Elicited peritoneal neutrophils and macrophages as well as alveolar macrophages and adherent splenic macrophages were used as representative cell types. The method to assess intracellular killing was based upon dye uptake and concentration by a dead micro-organism; methylene blue was used as the indicator dye and the test organism was Saccharomyces cerevisiae. Phagocytosis was measured by counting, microscopically, the number of ingested yeast (Saccharomyces cerevisiae) within the neutrophils or macrophages. A number of killed yeast, i.e., those which took up the dye, were readily visualized. The temporal pattern of phagocytosis and killing was determined concurrently. Splenic macrophages demonstrated the slowest phagocytic activity whereas neutrophils and alveolar macrophages manifested a similar phagocytic activity. Peritoneal macrophages exhibited a continuous increase in activity throughout the test period. Microbicidal activity was similar for all 4 cells types. The new technique for measuring phagocytosis and killing provides a rapid, inexpensive and easily quantified assay for assessing discrete phagocytic cell functions.

Animals↗
PubMed1978

Binding of benzo[a]pyrene at the 1,3,6 positions to nucleic acids in vivo on mouse skin and in vitro with rat liver microsomes and nuclei.

Loss of tritium from specific positions in [3H,14C] aromatic hydrocarbons can elucidate their binding site(s) to DNA and RNA and indicate the mechanism of activation. Studies of tritium loss from [6-3H,14C]benzo[a]pyrene (B[a]P), [1,3-3H,14C]B[a]P, [1,3,6-3H,14C]B[a]P, [6,7-3H,14C]B[a]P, and [7-3H,14C]B[a]P were conducted in vitro using liver nuclei and microsomes from 3-methylcholanthrene-induced Sprague-Dawley rats and in vivo on the skin of Charles River CD-1 mice. The relative loss of tritium from [3H, 14C]B[a]P was measured after binding to skin DNA and RNA, to nuclear DNA, and to native and denatured calf thymus and rat liver DNA's and poly(G) by microsomal activation. In skin, nuclei, and microsomes plus native DNA, virtually all B[a]P binding occurred at positions 1,3 and 6; while with microsomes plus denatured DNA or poly(G), B[a]P showed no binding at the 6 position and a small amount at the 1 and 3 positions. In vivo and with nuclei, binding at the 6 position predominated. Little loss of tritium from the 7 position was seen; this was expected because binding at this position is not thought to occur. This confirms the interpretation of loss of tritium as an indication of binding at a given position. These results demonstrate that the use of microsomes to activate B[a]P is not a valid model system for delineating the in vivo mechanism of B[a]P activation, and support previous evidence for one-electron oxidation as the mechanism of activation of hydrocarbons in binding to nucleic acids.

Animals↗