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Biomedical subjects

R Rogers

Publications and source records attributed to R Rogers.

At least 19 recordsLinked to original sources
PubMed1999

Evaluation of a novel dry latex preparation for demonstration of infectious mononucleosis heterophile antibody in comparison with three established tests.

A new, dried antigen-coated latex preparation for the demonstration of infectious mononucleosis (IM) heterophile antibody (Dryspot IM kit; Oxoid, Ltd., Basingstoke, Hampshire, United Kingdom) was compared with the IM kit (a liquid latex reagent from the same source), an immunoassay (ImmunoCard Mono; Meridian Diagnostics), and an absorption test (Monospot; Meridian Diagnostics). The latter was used as a standard for initial statistical comparisons. Discrepancies were resolved by using Epstein-Barr virus serology. Of the 328 routine samples tested, 77 were positive and 222 were negative by all IM heterophile antibody-based kits. Twenty-nine samples gave discrepant results. Following resolution of discrepant results, the sensitivity and specificity values for the IM Dryspot kit were 87.0 and 98.7%, those for the Oxoid liquid latex IM kit were 83.0 and 99.6%, and those for the ImmunoCard Mono immunoassay were 85.0 and 100.0%, respectively. The evaluation shows that the Dryspot kit, which is uniquely straightforward to use and may be stored at room temperature, is comparable in performance to other rapid heterophile tests for the confirmation of IM.

Antibodies, Heterophile↗
PubMed1999

Hsp60 is targeted to a cryptic mitochondrion-derived organelle ("crypton") in the microaerophilic protozoan parasite Entamoeba histolytica.

Entamoeba histolytica is a microaerophilic protozoan parasite in which neither mitochondria nor mitochondrion-derived organelles have been previously observed. Recently, a segment of an E. histolytica gene was identified that encoded a protein similar to the mitochondrial 60-kDa heat shock protein (Hsp60 or chaperonin 60), which refolds nuclear-encoded proteins after passage through organellar membranes. The possible function and localization of the amebic Hsp60 were explored here. Like Hsp60 of mitochondria, amebic Hsp60 RNA and protein were both strongly induced by incubating parasites at 42 degreesC. 5' and 3' rapid amplifications of cDNA ends were used to obtain the entire E. histolytica hsp60 coding region, which predicted a 536-amino-acid Hsp60. The E. histolytica hsp60 gene protected from heat shock Escherichia coli groEL mutants, demonstrating the chaperonin function of the amebic Hsp60. The E. histolytica Hsp60, which lacked characteristic carboxy-terminal Gly-Met repeats, had a 21-amino-acid amino-terminal, organelle-targeting presequence that was cleaved in vivo. This presequence was necessary to target Hsp60 to one (and occasionally two or three) short, cylindrical organelle(s). In contrast, amebic alcohol dehydrogenase 1 and ferredoxin, which are bacteria-like enzymes, were diffusely distributed throughout the cytosol. We suggest that the Hsp60-associated, mitochondrion-derived organelle identified here be named "crypton," as its structure was previously hidden and its function is still cryptic.

Alcohol Dehydrogenase↗
PubMed1998

Characterization and chromosomal localization of PTP-NP-2, a new isoform of protein tyrosine phosphatase-like receptor, expressed on synaptic boutons.

Recently, there have been several reports describing the cloning and characterization of the novel family of protein tyrosine phosphatase-like receptor molecules (known as IA-2 and PTP-NP/PTP-IAR/IA-2beta/phogrin), which may act as autoantigens in diabetes. Here, we report the molecular characterization and chromosomal localization of a new isoform of this family in brain termed PTP-NP-2 (for PTP-NP tyrosine phosphatase isoform), and its function in rat primary hippocampal neurons. PTP-NP-2 has 48% identity to IA-2. The principal difference between PTP-NP-2 and PTP-NP is a 17-amino-acid insert near the N-terminus of PTP-NP that is absent in PTP-NP-2. Genomic DNA analysis indicates that the 17-amino-acid insert is coded by a separate exon, suggesting that both IA-2beta and PTP-NP-2 are isoforms arising by alternate splicing of the same gene. Reverse transcriptase-PCR revealed that both isoforms are present in human SH-SY5Y neuroblastoma cells. PTP-NP-2 mRNA expression is highly restricted, with a 5.5-kb specific transcript in human fetal and adult brain and 5.5 and 3. 8 kb in human adult pancreas. SH-SY5Y neuroblastoma and U87-MG glioblastoma cells showed specific transcripts of 5.5 and 3.8 kb, respectively, indicating the existence of several isoforms of this molecule in the nervous system. The human gene encoding PTP-NP-2 was assigned to human chromosome 7q22-qter using Southern blot analysis of genomic DNAs from rodent/human somatic hybrid cell lines. Confocal microscopy analyses of rat primary hippocampal neurons revealed that PTP-NP-2 is abundantly expressed on synaptic boutons in primary neurons. Wild-type PTP-NP-2 showed no measurable tyrosine phosphatase activity using an in-vitro pNPP assay. Examination of the PTP-NP-2 catalytic consensus sequence revealed that this sequence differed from the typical tyrosine phosphatase-domain consensus sequence by an alanine to aspartate change (amino acid 930). Mutation of aspartate 930 to alanine produced a catalytically active enzyme, suggesting that native PTP-NP and its isoform PTP-NP-2 are catalytically inactive receptor protein tyrosine phosphatase homologues. Taken together, these results indicate that the tyrosine phosphatase PTP-NP-2 is a new isoform of PTP-NP tyrosine phosphatase, is expressed on synaptic boutons and may participate in the regulation of synaptic bouton endocytosis.

Adult↗
PubMed1998

NRP/B, a novel nuclear matrix protein, associates with p110(RB) and is involved in neuronal differentiation.

The nuclear matrix is defined as the insoluble framework of the nucleus and has been implicated in the regulation of gene expression, the cell cycle, and nuclear structural integrity via linkage to intermediate filaments of the cytoskeleton. We have discovered a novel nuclear matrix protein, NRP/B (nuclear restricted protein/brain), which contains two major structural elements: a BTB domain-like structure in the predicted NH2 terminus, and a "kelch motif" in the predicted COOH-terminal domain. NRP/B mRNA (5.5 kb) is predominantly expressed in human fetal and adult brain with minor expression in kidney and pancreas. During mouse embryogenesis, NRP/B mRNA expression is upregulated in the nervous system. The NRP/B protein is expressed in rat primary hippocampal neurons, but not in primary astrocytes. NRP/B expression was upregulated during the differentiation of murine Neuro 2A and human SH-SY5Y neuroblastoma cells. Overexpression of NRP/B in these cells augmented neuronal process formation. Treatment with antisense NRP/B oligodeoxynucleotides inhibited the neurite development of rat primary hippocampal neurons as well as the neuronal process formation during neuronal differentiation of PC-12 cells. Since the hypophosphorylated form of retinoblastoma protein (p110(RB)) is found to be associated with the nuclear matrix and overexpression of p110(RB) induces neuronal differentiation, we investigated whether NRP/B is associated with p110(RB). Both in vivo and in vitro experiments demonstrate that NRP/B can be phosphorylated and can bind to the functionally active hypophosphorylated form of the p110(RB) during neuronal differentiation of SH-SY5Y neuroblastoma cells induced by retinoic acid. Our studies indicate that NRP/B is a novel nuclear matrix protein, specifically expressed in primary neurons, that interacts with p110(RB) and participates in the regulation of neuronal process formation.

Adult↗
PubMed1998

An analysis of competency to stand trial: an integration of case law and clinical knowledge.

In the past three decades competency to stand trial has emerged as a central issue in mental health law and forensic psychology. This article combines legal and clinical frameworks for a more integrated understanding of competency to stand trial. The legal framework focuses on Supreme Court decisions beginning with Dusky and reviews several legal theories of competence. The empirical framework is investigated within Grisso's (1986) conceptualization of legal competencies. In addition, the efficacy of specialized forensic assessments is evaluated, including the newly developed MacArthur Structured Assessment of the Competencies of Criminal Defendants (MacSAC-CD). The article concludes with observations of competency as a construct and their implications for forensic practice.

Clinical Competence↗
PubMed1998

Overcoming the barriers: national to European to G7.

An information revolution is underway which will have an impact on all sectors of society. It will fundamentally change national and international health systems. The global Internet is a key influence and will change the balance of power within and between healthcare professions, and between them and the general public. This revolution offers enormous potential benefits to global health but there is also potential for harm. There are a wide range of barriers to realising the potential benefits. They lie in areas such as the protection of personal information; ownership and legal accountability; data meanings: structures and database navigation; deficiencies in the global Internet and lack of access by many communities. This paper considers the nature of those barriers. In 1994, the Group of Seven Nations launched an initiative to stimulate a global information society. Theme 8 deals with healthcare and therein Sub-project 5 'Enabling Mechanisms', which the author leads, is seeking to identify barriers and the authoritative international sources of advise and good practice. It is conducting an international survey, the results of which should be published by the end of 1997. This paper describes the aims of this Sub-project.

Computer Communication Networks↗
PubMed1998

Assessment of malingering with simulation designs: threats to external validity.

Comprehensive forensic evaluations are predicated on the accurate appraisal of response styles that may affect evaluatees' clinical presentation and experts' conclusions associated with psycholegal issues. In the assessment of malingering, forensic experts often rely heavily on standardized measures that have been validated exclusively via analogue research. While such research augments internal validity, the threats to external validity are readily apparent. As the first study of these threats, type of incentive (positive versus negative), context (a familiar versus unfamiliar scenario), and relevance to the participants was investigated systematically with a between-subjects factorial design. A sample of 231 undergraduates was asked to either (a) feign major depression and given an easily understood description of this disorder or (b) serve as controls responding honestly. They were administered a brief measure of psychopathology (Hopkins Symptom Checklist; Derogatis, Lipman, Rickels, Uhlenhuth, & Covi, 1974) and a recent screen for malingering (Screening Inventory of Malingered Symptoms or SIMS; Smith, 1992) in 1 of 18 experimental conditions. Results suggested that incentive had a main effect on the SIMS. More specifically, simulators under negative incentives appeared more focused in their feigning; they produced more bogus depressed symptoms, but fewer symptoms unrelated to depression. Interactions were also observed between context and incentive, and context and relevance. Implications of these results are explored for both analogue research on malingering and current forensic practice.

Adult↗
PubMed1998

A comparison of forensic and nonforensic malingerers: a prototypical analysis of explanatory models.

Explanatory models of malingering strive to understand the primary motivation underlying attempts to feign. Rogers, Sewell, and Goldstein (1994) provided empirical support for the conceptualization of pathogenic, criminological, and adaptational models. In the current study, a prototypical analysis of 221 forensic experts results in a slightly refined formulation: the adaptational models was decomposed into its two broad dimensions (cost-benefit analysis and adversarial setting). An important findings is that the factor structure for the explanatory models remained stable when applied to both forensic and nonforensic cases. As a first investigation, significant differences were observed in prototypical cases of malingering by the category of referral (forensic or nonforensic) and by type of feigning (mental disorders, cognitive impairment, and medical syndromes). Surprisingly, the feigning of medical syndromes appeared to play a relatively prominent role in both forensic and nonforensic cases and to be influenced by the apparent adversarial context of the assessment. Finally, important gender differences were observed, especially with nonforensic prototypical cases of malingering.

Adaptation, Psychological↗
PubMed1998

Psychopathy and recidivism among female inmates.

Despite a plethora of studies investigating psychopathy among male offenders, little is known about the applicability of this construct to female populations. Research has shown that prevalence rate, symptom presentation, and diagnostic comorbidity differ for females as compared to males. The current study is the first to examine the relationship between psychopathy and recidivism among women. Recidivism data on a sample of 78 female inmates were examined at a 1-year interval in relation to the Psychopathy Checklist-Revised (PCL-R), inclusion criteria for the Antisocial Personality Disorder Diagnosis from the Personality Disorder Examination (PDE), and selected scales from the Personality Assessment Inventory (PAI; Antisocial and Aggression scales). The egocentricity subscale of the PAI, Factor 1 of the PCL-R, and the verbal aggression subscale of the PAI were the best predictors of future recidivism. Specific differences emerged between male and female offenders when comparing the present data with previous studies of male psychopaths.

Adult↗
PubMed1998

Blood ammonia--heart rate relationship during graded exercise is not influenced by glycogen depletion.

The blood lactate (La) - heart rate (HR) relationship assessed during a graded maximal exercise test (GXT) is often used to provide training guidelines to athletes. Glycogen depletion is a condition that is known to influence the La response during a GXT and therefore can bias the interpretation of La curves. The purpose of our study was to determine an influence of glycogen depletion through long-term exercise (GDE) on blood La and ammonia (NH3) accumulation during a GXT. Eleven persons (5 males, 6 females; 26.3 +/- 4 yrs) performed a cycle ergometer GXT (3 min protocol) on two separate occasions with a 1-week interval. Using a randomized cross-over design, one of the two GXTs was preceded by a GDE. After GDE, blood NH3 increased more rapidly in the depleted versus control state whereas La increased less rapidly in the depleted state. The NH3 to HR relationship was not altered by glycogen depletion. We conclude, therefore, that determination of NH3 during a GXT may provide helpful additional information for training recommendations which is not available through La determination only.

Adult↗
PubMed1998

Validity and reliability in a Flemish population of the WHO-MONICA Optional Study of Physical Activity Questionnaire.

PURPOSE: This study assessed the validity and reliability of a self-administered physical activity questionnaire used in the WHO-MONICA project. METHODS: Subjects (N = 167) were physical education and physical therapy alumni. The questionnaire (MOSPA-Q) covers a 1-yr period and measures the average weekly time and energy expenditure spent in one's occupation, transport-related activities, household chores, and leisure time activities. Test-retest reliability was reported as the intra-class correlation between calculated time or energy expenditure in different questionnaire items determined from two MOSPA-Q administrations. Validity was assessed using biometrical and physiological parameters as criterion measures. RESULTS: Test-retest reliability ranged from 0.45 to 0.92 with the highest correlation coefficients obtained for the leisure time physical activities. The correlation between calculated daily total energy expenditure (TEE, Kcal.d-1) and lean body mass was 0.53 (P < 0.001). The correlation between energy expenditure in leisure time activities (Kcal.wk-1) and VO2peak was 0.44. CONCLUSIONS: The MOSPA-Q provides a relatively valid and reliable estimate of physical activity.

Adult↗
PubMed1998

Convergent validity of the personality assessment inventory: A study of emergency referrals in a correctional setting.

The Personality Assessment Inventory (PAI) is a new generation multiscale inventory that is widely used in clinical settings. The current study examined its convergent validity with emergency referrals in a metropolitan correctional facility. A sample of 80 referrals completed two phases of an assessment study. As a measure of convergent validity, results on select PAI scales were compared to the Schedule of Affective Disorders and Schizophrenia (SADS), the Structured Interview of Reported Symptoms (SIRS), and the Suicide Probability Scale (SPS). Overall, results indicated moderate to good convergent validity for the following: (a) screening for feigned profiles, (b) establishing clinical correlates of common disorders, and (c) evaluating the potential for suicidal ideation.

Adult↗
PubMed1998

The PAI and feigning: A cautionary note on its use in forensic-correctional settings.

Indicators of feigned PAI profiles were derived from comparisons of simulators instructed to feign and genuine patient groups. Concerns are raised regarding whether these indicators are applicable to forensic and correctional populations and can be cross-validated with a known-groups comparison. Compiling data on 57 malingerers and 58 genuine patients from two forensic and correctional sites, three primary indicators of feigning, Negative Impression (NIM) scale, Malingering Index, and the Rogers Discriminant Function (RDF) were investigated. Results suggested that the RDF was not applicable to forensic referrals. However, NIM 77T appeared to be a useful screen for forensic samples. In addition, convergent evidence of feigning was found across designs (simulation and known-groups) and samples (non-forensic and forensic) for extreme elevations on NIM (>/=110T) and Malingering Index (>/=5).

Adult↗
PubMed1998

Preliminary validation of the MMPI-A for a male delinquent sample: an investigation of clinical correlates and discriminant validity.

Adolescent psychopathology, until recently, has been a largely neglected area of research and poses unique challenges for psychological assessments. In response to the specific need for adolescent-focused measures, the MMPI-A (Butcher et al., 1992) was among several measures to be developed. Although a sizeable literature exists on the original MMPI (Hathaway & McKinley, 1943) and adolescent populations, relatively few empirical studies have been published on the MMPI-A. The primary purpose of this study was the examination of clinical correlates for the MMPI-A for a male delinquent sample. MMPI-A protocols were collected from 99 adolescents at a North Texas juvenile correctional facility, and systematic comparisons were conducted between the Basic Scales and symptoms/diagnoses derived from the Schedule of Affective Disorders and Schizophrenia for School-Age Children (K-SADS-III-R; Ambrosini, Metz, Prabucki, & Lee, 1989). Using only K-SADS-III-R symptoms with high reliabilities (rs > .80), a comprehensive list of correlates was generated for the Clinical, Supplementary, and Content Scales. Additionally, stepwise discriminant functions successfully classified MMPI-A protocols according to K-SADS-III-R diagnoses. As an exploratory analysis, ethnic differences on MMPI-A profiles were also investigated, revealing significant differences among groups.

Adolescent↗
PubMed1998

Reducing the risk of hepatitis to staff.

Hepatitis continues to pose a serious risk to the health of staff and patients. A number of procedures can be put in place to protect the health and safety of nurses exposed to the risk. Nurses should be aware of systems and policies in their own work area.

Cross Infection↗
PubMed1998

Radiation therapy for the management of biopsy proved local recurrence after radical prostatectomy.

PURPOSE: We determine which clinical characteristics correlate with a successful outcome following external beam radiation for the management of biopsy proved, locally recurrent prostate cancer after radical prostatectomy. MATERIALS AND METHODS: The clinical records of 34 patients who were treated at our institution with external beam radiation for biopsy proved local disease recurrence after radical prostatectomy were reviewed. Mean followup was 77.9 months after radical prostatectomy and 38.3 months after radiotherapy. Preoperative, postoperative and pathological characteristics were examined for the ability to predict failure following radiotherapy. RESULTS: Of the 34 patients 9 (26%) exhibited persistently low and stable serum prostate specific antigen (PSA) (less than 0.5 ng./ml.), while 7 (21%) had an undetectable serum PSA (less than 0.1 ng./ml.) during followup. These 16 patients were considered to be successfully treated by radiation and in the remaining 18 (53%) radiation was considered to have failed. The likelihood of successful treatment at 3 years after radiotherapy for all patients was 48%. Preoperative PSA, PSA at first elevation, postoperative PSA velocity and pathological stage were not significant predictors of a successful outcome following radiation treatment. Patients with a serum PSA of 4 ng./ml. or less before receiving radiotherapy and those with a prostatectomy specimen Gleason score of 7 or less were significantly more likely to be successfully treated by radiotherapy. CONCLUSIONS: Radiation therapy is a viable treatment option for select patients with biopsy proved local disease recurrence following radical prostatectomy. The chance of achieving and maintaining a persistently low and stable or undetectable serum PSA is likely in those patients with a prostatectomy specimen Gleason score of 7 or less and a pre-radiation PSA of 4.0 ng./ml. or less. More effective treatment regimens are needed for those patients in whom radical prostatectomy fails.

Aged↗
PubMed1998

Differences in anxiety between first-time and multiple-time inmates: a multicultural perspective.

The present study investigated differences in anxiety symptoms and associated features between 129 first-time and multiple-time male inmates from a large urban jail. Participants were assessed on three separate anxiety measures: the Structured Clinical Interview of DSM-III-R Disorders (SCID) anxiety disorders module, the State Trait Anxiety Inventory, and anxiety scales of the Personality Assessment Inventory (PAI). First-time inmates were found to have more symptom severity for Generalized Anxiety Disorder, especially symptoms related to scanning and vigilance. Both first-time and multiple-time inmates evidenced higher state than trait anxiety, although this pattern did not hold for African Americans. Contrary to previous research, few ethnic differences were found in this incarcerated sample.

Adult↗