Search PubMed⌕ Search

Biomedical subjects

R Robinson

Publications and source records attributed to R Robinson.

352 records · Page 20Linked to original sources
PubMedDate not supplied

Question time.

Explore the source record for details and available documents.

Adaptation, Psychological↗
PubMedDate not supplied

[Seafood poisoning in Madagascar: current state of knowledge and results of a retrospective study of the inhabitants of coastal villages].

In 1996 and 1997, a knowledge, attitude and practice survey concerning seafood poisonings was conducted in 560 villages spread along the Madagascar coasts, gathering 585,000 people. 175 serious and 205 mild seafood poisonings after fish, shark and turtle meals occured during the period 1930 to 1996. Squales (mainly Sphyrnidae and Cacharinidae familiesi) are the most often responsible of serious poisoning (48% of episodes), then other fishes (37%), and mainly of the Clupeidae family (herrings, sardinels), then marine turtles (11%), with Eretmochelys imbricata and Chelonia mydas, and finally crabs (4%). Neurological symptoms are predominant in squale poisonings, neurological symptoms associated with gastrointestinal symptoms are present in 50% of all kind of seafood poisoning episods. Most of episods incame on the East Coast (mainly Toamasina and Antisiranana Region) and on the South-West Coast (Toliara Region). Mild seafood poisonings are spread along all the Coasts but central East Coast; fishes are the most often responsible (41% of episodes). Gastro-intestinal symptoms are the most conmon. More than 50% of t interviewed people knows about poisoning risks with some kind of marine animals, but less than 20% practice preventive measures such as giving a piece of fished animal to a domestic animal before eating. These results are used to plan a comprehensive epidemiological surveillance and control programme.

Foodborne Diseases↗
PubMedDate not supplied

Child's play.

Explore the source record for details and available documents.

Child↗
PubMedDate not supplied

A procedure for the rapid separation and purification of UDP-glucuronosyltransferases from rabbit liver microsomes.

A technique has been developed which rapidly separates and purifies UDP-glucuronosyltransferases from liver microsomes of untreated rabbits. by use of this method, highly purified estrone and p-nitrophenol UDP-glucuronosyltransferases can be obtained in good yield in about 48 hr. Microsomes were solubilized with the nonionic detergent Emulgen 911 in low ionic strength buffers and applied to a DEAE-cellulose column equilibrated with low ionic strength buffers. UDP-glucuronosyltransferase activities were then eluted in a stepwise fashion with increasing concentration of KCl. Three fractions were studied. The first two fractions contained only estrone UDP-glucuronosyltransferase activity while a third contained p-nitrophenol UDP-glucuronosyltransferase activity. Each fraction was directly applied to a UDP-hexanolamine Sepharose-4B column, which was then washed extensively with KCl, and the transferases were eluted with UDP-glucuronic acid. A method for separating the transferases on the affinity column is presented. Testosterone and morphine could not be conjugated by any of the purified enzymes.

Animals↗
PubMedDate not supplied

Evaluation of Ki-67 (MIB-1) in placentas with hydropic change and partial and complete hydatidiform mole.

The separation of complete from partial hydatidiform mole and of partial mole from placentas with hydropic change on gross and microscopic evaluation can be difficult, and ploidy provides important diagnostic information. We applied an immunohistochemical marker of proliferation, Ki-67 (MIB-1), to 10 complete moles, 11 partial moles, and 8 placentas with hydropic change to determine whether growth fraction differs in these three placentas and can aid in diagnosis. Ploidy was confirmed using flow cytometry and fluorescence in situ hybridization with probes to chromosomes 7 and 2. The Ki-67-determined growth fractions (number of positive cells/total number of cells) for villous stromal cells, cytotrophoblast, and proliferating trophoblast were evaluated separately by counting 200 cells of each population. Growth fraction on stroma did not differ among the three entities. Mean percent growth fraction for cytotrophoblast was 13.3% for hydropic change, 14.6% in partial moles and 38.7% in complete moles (P = .004 hydropic change to complete moles, P = .003 partial moles to complete moles). There was no significant difference between hydropic change and partial mole. Mean percent growth fraction for proliferating trophoblast was 38.5% in hydropic change, 25.9% in partial moles, and 67.1% in complete moles (P = .08 hydropic change to complete moles, P = .004 partial moles to complete moles). Again, no significant difference was identified between hydropic change and partial moles. Ploidy analysis using fluorescence in situ hybridization and flow cytometry confirmed diploidy in hydropic change and complete moles and triploidy in partial moles. Ki-67 may be useful in separating complete moles from partial moles but not partial moles from hydropic change.

Cell Division↗