Search PubMed⌕ Search

Biomedical subjects

R Robinson

Publications and source records attributed to R Robinson.

At least 253 records · Page 14Linked to original sources
PubMed1977

An analysis of nucleotides and catecholamines in bovine medullary granules by anion exchange high pressure liquid chromatography and fluorescence. Evidence that most of the catecholamines in chromaffin granules are stored without associated ATP.

The catecholamine and nucleotide content of chromaffin granules from bovine adrenal medulla was analyzed both qualitatively and quantitatively. Nucleotides were examined by separation on anion exchange high pressure liquid chromatography and ultraviolet absorption, as well as by anion exchange thin-layer chromatography and luciferin-luciferase light emission analysis. CPATHECHOLAMINES WERE QUANTIFIED VIA AUTOMATED CONTINUOUS-FLOW ANALYSIS USING FLUORESCENCE. We found the average catecholamine/ATP ratio to be 8.2:1 with an SD of 1.98 and an SE of 0.50 for 16 separate glands. Relative percentages of epinephrine and norepinephrine were found to be 79.8 and 20.2, respectively. The granules contained 80.9% ATP, 12.3% GTP, and 6,8% UTP, as well as detectable amounts of ADP, AMP, GDP, and UDP. If we accept the catecholamine/ATP ratio of 4:1 proposed by Hilliarp earlier, then those catecholamines constituting the remainder of our ratio of 8.2:1 should represent a nucleotide-unassociated pool. In view of recent evidence of a direct agonistic function of nucleotides, we propose that the granule-associated nucleotides may act locally as coagonists with certain biogenic amines, and may additionally provide acirculating pool of purines and pyrimidines for use by the heart and lungs.

Adenosine Triphosphate↗
PubMed1976

Genetic studies of the Syrian hamster. X. Rex.

The rex mutant has its most obvious effect on coat structure but it also reduces 21-day body weight by about 14 per cent. There is no apparent effect on viability nor on the fecundity of the more robust rex females. Extensive tests for linkage with the genes b, Ba, cd, Ds, e l, Lg, ru, Sa and Wh have proved to be negative. A less extensive test with s was also negative.

Animals↗
PubMed1976

President's address.

Explore the source record for details and available documents.

Academies and Institutes↗
PubMed1975

A simple chromatographic procedure for the concurrent estimation of urinary 4-hydroxy-3-methoxymandelic acid (HMMA) and homovanillic acid (HVA) using a scanning technique.

A rapid and simple procedure is described for the concurrent determination of 4-hydroxy-3-methoxymandelic acid (HMMA) and homovanillic acid (HVA). The acids are separated by chromatography and estimated by a scanning technique. The urinary output of HMMA and HVA over 24 h from 123 normals (80 males, 43 females) gave mean values of 3.3 +/- 0.94 and 4.9 +/- 1.3 mg, respectively. There was no significant difference in the 24 hour urinary output of HMMA and HVA between the male and femal groups.

Chromatography, Paper↗
PubMed1975

Diagnosis of chronic and postoperative osteomyelitis with gallium 67 citrate scans.

The effectiveness of gallium 67 citrate in diagnosing the exacerbations of chronic osteomyelitis or postoperative osteomyelitis secondary to open reduction of fractures was tested in thirteen patients. This isotope demonstrated an area of infection in every case in which such an area existed. The control subjects consisted of patients with noninfected wounds after open reduction. In this group, gallium was not incorporated into the post-operative hematoma. No false-negative results were observed in our series, demonstrating that this element is useful in the early diagnosis of bone infections.

Adult↗
PubMed1975

Variations in mechanical sampling: their causes and effects.

Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error.

Autoanalysis↗
PubMed1974

Clinical infections detected by 67Ga scanning.

Gallium-67 was utilized in 34 patients suffering from clinically demonstrable infections. The isotope was able to localize infections in soft tissue, bones, and some areas of the abdomen. Its utilization in the diagnosis of pelvic inflammatory disease is complicated by the fact that the isotope is secreted into the bowel and obscures the area. This isotope is ideal for the diagnosis of acute and/or chronic inflammation of bone secondary to open reduction of fractures, and may also be of use in the diagnosis of primary osteomyelitis.

Abscess↗